Effect of Fibronectin or Laminin on Liver Regeneration following Hepatectomy.

1)本文观察了肝硬化（LC）患者和大鼠血浆纤维连接蛋白（FN）水平与肝切除术后残肝再生过程的关系。人和大鼠肝切除术后血浆FN水平立即降低。正常肝脏的患者和大鼠分别在一个月和一周内恢复到术前血浆FN水平，但LC患者血浆FN水平较低的时间更长。FN水平与肝再生程度密切相关。这些结果表明血浆FN水平作为肝再生的参数的有用性。2)此外，我们研究了FN或抑肽酶补充对大鼠肝切除后肝再生过程中的作用。70%肝切除后，大鼠立即腹腔注射纯化的人血浆FN和/或抑肽酶。给予FN和抑肽酶可显著增加肝切除术后^3H-胸苷摄取、网状内皮系统吞噬指数、有丝分裂指数和肝再生率，但单独注射抑肽酶对这些指标无显著促进作用。另一方面，补充吲哚美辛的肝再生导致显着抑制氚化胸苷的摄取，肝切除术后24小时，克服了FN和抑肽酶对DNA合成的刺激作用。这些结果表明，FN可能作为网状内皮系统的激活剂，特别是在激活枯否细胞，刺激释放的胡萝卜素促进DNA合成的过程中的肝再生，抑肽酶也可能通过抑制FN的蛋白水解增强FN的效果。3)在硫代乙酰胺诱导的肝硬化大鼠中，补充FN和抑肽酶也增加了肝切除后24小时的DNA合成，但低于正常肝大鼠。

1) The relationship between plasm fibronectin (FN) levels and the regenerative process in liver remnants after hepatectomy was studied in patients and in rats with or without liver cirrhosis (LC). Plasma FN levels were reduced immediately after hepatectomy in human and rat. Both patients and rats with normal livers returned to the preoperative plasma FN livels within one month and one week, respectively, but low levels of plasma FN persisted longer in those with LC. FN levels were well correlated with the degree of the hepatic regeneration. These results suggest the usefulness of plasma FN levels as a parameter for liver regeneration. 2) Moreover, we studied the role of FN or aprotinin supplementation on the process of liver regeneration following hepatectomy in rats. After 70% hepatectomy, rats were immediately given purified human plasma FN and/or aprotinin intraperitoneally. The admi nistration of FN and aprotinin significantly increased ^3H-thymidine uptake, the phagocytic index of the reticuloendothelial system, the mitotic index, and the liver regeneration rate following hepatectomy, but the injection of aprotinin alone did not significantly promote those indices. On the other hand, the supplementation of indomethacin for the liver regeneration resulted in significant suppression of the tritiated thymidine uptake, 24 hours after hepatectomy, and overcame the stimulative effect of FN and aprotinin on DNA synthesis. These results suggest that FN may act as an activator of the reticuloendothelial system, and especially on the activation of Kupffer cells, which stimulate the release of prostaglandins promoting DNA synthesis in the process of liver regeneration, and, also aprotinin may enhance the effect of FN by inhibiting the proteolysis of FN. 3) In the liver cirrhosis rats induced by thioacetamide, the supplementation of FN and aprotinin also increased DNA synthesis, 24 hours after hepatectomy, but lower than those in normal liver rats.