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CHOLINERGIC FUNCTIONS FOR BRAIN PLASTICITY DURING BIRD SONG LEARNING

鸟鸣学习过程中大脑可塑性的胆碱能功能

基本信息

批准号：
05680703
负责人：
SAKAGUCHI Hironobu
金额：
$ 1.02万
依托单位：
Dokkyo University, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680703/
关键词：
Zebra finch Sensitive period Song learning Brain plasticity Acetylcholine PI turnover Protein kinase C GAP-43 イノシトール大謝

项目摘要

A young male zebra finch learns its song during a sensitive period. The sensory motor phase of song learning is closely correlated to development of a song control nucleus, RA,which receives inputs from two other song control nuclei. We previously found that acetylcholine (ACh) content and the enzyme activity of choline acetyltransferase (ChAT) increase transiently in the RA during a sensitive period of song learning, when RA synapses for two inputs are highly plastic. For this purpose, we have investigated whether muscarinic agonist carbachol would affect phosphoinositide (PI) metabolism in the RA.The male zebra finch at 30 days, 50 days after hatching and adults were used. Carbachol-stimulated PI turnover was examined by measuring the accumulation of [3H]-inositol phosphates from [3H]-inositol labeled synaptoneurosomes of RA,in the presence of lithium. Carbachol increased PI turnover significantly. Carbachol-stimulated PI turnover reached a maximum at 50 days after hatching, and decreased to adult level. These results suggest that ACh affects synaptic plasticity of zebra finch RA during song learning, because PI turnover activate protein kinase C responsible for the phosphorylation of some phosphoproteins, which play a important role in synaptic plasticity.

一只年轻的雄性斑胸草雀在一个敏感的时期学习它的歌曲。鸣唱学习的感觉运动阶段与鸣唱控制核（RA）的发育密切相关，RA接收来自其他两个鸣唱控制核的输入。我们以前发现，乙酰胆碱（ACh）的含量和胆碱乙酰转移酶（ChAT）的酶活性增加短暂的RA在敏感期的歌曲学习，当RA突触的两个输入是高度可塑性的。为此，我们研究了毒蕈碱激动剂卡巴胆碱是否会影响RA的磷酸肌醇（PI）代谢。在锂的存在下，通过测量来自[3 H]-肌醇标记的RA突触神经体的[3 H]-肌醇磷酸的积累来检查卡巴胆碱刺激的PI周转。卡巴胆碱增加PI营业额显着。氨甲酰胆碱刺激的PI周转在孵化后50天达到最大值，并下降到成人水平。这些结果表明，ACh影响斑胸草雀鸣唱学习过程中的突触可塑性，这是因为PI的周转激活了负责磷酸化某些磷酸化蛋白的蛋白激酶C，这些磷酸化蛋白在突触可塑性中起重要作用。