Sterile effect of benzoylphenylurea type insect growth regulator

苯甲酰基苯脲(BPU)化合物对昆虫具有独特的生理效应。当用氟虫隆(CF)(一种BPU化合物)处理昆虫幼虫时，昆虫在蜕皮阶段被杀死。CF处理在产卵卵中也表现出不育效果。当处理雄虫幼虫时，这种影响更为严重。本试验主要利用斜纹夜蛾。(1)用CF处理斜纹夜蛾幼虫，雄虫的不孕效应比雌虫严重。在小菜蛾中也观察到了同样的现象。(2)5龄幼虫经CF处理后，6龄第3天以后，处理组幼虫的精巢明显小于对照组。(3)用CF处理5龄幼虫时，处理后的蛹和成虫的无核精子束和真核精子束数目较少。CF处理也延缓了精子从睾丸到输精管的运动。(4)CF处理不影响交配行为。然而，转移到雌性体内的无核精子束数量却减少了。无性精子束数与受精卵的不育率呈高度正相关。(5)CF处理对睾丸蜕皮激素效价有影响。其不孕作用可能与蜕皮激素效价降低有关。应进一步研究CF对蜕皮激素效价的影响。通过这类工作，有可能解决BPU抑制甲壳素合成的作用方式。

Benzoylphenylurea (BPU) compounds show a unique physiological effect to insects. When insect larvae are treated with chlorfluazuron (CF) (One of BPU compounds), insects are killed at molting stages. CF treatment also showed a sterile effect in the oviposited eggs. This effect was more serious when male larvae wee treated. Spodoptera litura was mainly used in this experiment. (1) When larvae of S.litura were treated with CF, sterility effect was observed more seriously in males than females. The same phenomenon was also observed in Plutella xylostella. (2) When 5th instar larvae wee treated with CF, the size of testis was observed to be smaller in CF treated larvae than those of the control ones after stages of 3rd day of 6the instar. (3) When 5th instar larvae were treated with CF, the numbers of apyrene and eupyrene sperm bundles were smaller in treated pupae and adults. The movements of sperm from testis to vas diference was also delayd by CF treatment. (4) CF treatment did not affect mating behavior. However, the number of apyrene sperm bundles transferred to females was decreased. There was a high correlationship between the number of apyrene sperm bundle and sterility effect of oviposited eggs. (5) CF treatment affected the amount of ecdisteroid titer of testis. The sterility effect of CF was thought to be involved in the decrease of ecdisteroid titer. Further studies how CF affect ecdisteroid titer should be performed. There might be a possibility that the mode of action of BPU on the chitin synthesis inhibition will be solved through this kind of work.