权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Sterile effect of benzoylphenylurea type insect growth regulator

苯甲酰基苯脲类昆虫生长调节剂的杀菌作用

基本信息

批准号：
05806006
负责人：
MIYATA Tadashi
金额：
$ 1.15万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05806006/
关键词：
Benzoylphenylurea compound Chlorfluazuron Ecdisteroid Testis Eupyrene and apyrene sperm Insect growth regulator Inhibition of egg hatching Sterile effect 無核精子有核精子ハスモンヨトウベンゾイルフェニルウレア

项目摘要

Benzoylphenylurea (BPU) compounds show a unique physiological effect to insects. When insect larvae are treated with chlorfluazuron (CF) (One of BPU compounds), insects are killed at molting stages. CF treatment also showed a sterile effect in the oviposited eggs. This effect was more serious when male larvae wee treated. Spodoptera litura was mainly used in this experiment. (1) When larvae of S.litura were treated with CF, sterility effect was observed more seriously in males than females. The same phenomenon was also observed in Plutella xylostella. (2) When 5th instar larvae wee treated with CF, the size of testis was observed to be smaller in CF treated larvae than those of the control ones after stages of 3rd day of 6the instar. (3) When 5th instar larvae were treated with CF, the numbers of apyrene and eupyrene sperm bundles were smaller in treated pupae and adults. The movements of sperm from testis to vas diference was also delayd by CF treatment. (4) CF treatment did not affect mating behavior. However, the number of apyrene sperm bundles transferred to females was decreased. There was a high correlationship between the number of apyrene sperm bundle and sterility effect of oviposited eggs. (5) CF treatment affected the amount of ecdisteroid titer of testis. The sterility effect of CF was thought to be involved in the decrease of ecdisteroid titer. Further studies how CF affect ecdisteroid titer should be performed. There might be a possibility that the mode of action of BPU on the chitin synthesis inhibition will be solved through this kind of work.

苯甲酰苯脲类化合物对昆虫具有独特的生理作用。当用氯氟脲（一种BPU化合物）处理昆虫幼虫时，昆虫在蜕皮阶段被杀死。CF处理对受精卵也有不育作用。这种影响在雄幼虫处理后更为严重。本实验主要采用斜纹夜蛾。(1) CF处理斜纹夜蛾幼虫时，雄性不育效应较雌性严重。在小菜蛾中也观察到同样的现象。(2) CF处理5龄幼虫，6龄第3天时，CF处理的幼虫睾丸尺寸小于对照。(3) CF处理5龄幼虫时，蛹和成虫的apyrene和eupyrene精子束数量较少。CF治疗也延迟了精子从睾丸到输精管的运动。(4) CF处理不影响交配行为。然而，转移给雌性的apyrene精子束数量减少。无炔精束数量与受精卵的不育效果密切相关。(5) CF治疗对睾丸激素滴度有影响。CF的不育作用被认为与睾酮滴度的降低有关。CF对睾酮滴度的影响有待进一步研究。通过这项工作，有可能解决BPU对几丁质合成抑制的作用方式。