THE RELATIONSHIP BETWEEN BACTERIAL SUPERANTIGEN AND PERIODONTITIS.

研究了化脓性链球菌产生的致热链球菌外毒素(SPE)的生物活性与牙周炎的关系，以及口腔链球菌产生的丝裂原因子(MF)的生物活性与牙周炎的关系。对SPE进行了纯化，建立了检测这些毒素的酶联免疫吸附试验。这些SPE可向人外周血单核细胞(PBMC)增殖，并促进携带TCR Vbeta2的T细胞生长。检测SPEs处理的PBMC培养上清液中的肿瘤坏死因子活性和IL-2的产生，并诱导PBMC表达肿瘤坏死因子-α、肿瘤坏死因子-β、白介素2和干扰素-γ。此外，我们还检测了血管紧张性链球菌培养上清液中的MF，并对该因子进行了部分纯化。结果表明，从口腔分离的化脓性链球菌或链球菌分离的SPE或MF可能与牙周炎的发生有关。

The relationship between biological activities of streptococcal pyrogenic exotoxins (SPE) produced by Streptococcus pyogenes and periodontitis, between biological activities of mitogenic factor (MF) from oral streptococci and periodonttitis were studied. SPEs were purified, and ELISA for detection of these toxins was achieved. These SPEs proliferated to human periferal blood mononuclear cells (PBMC), and promoted to growth T cell bearing TCR Vbeta2. TNF activity and IL-2 production were detected in culture supernatant of PBMC treated with SPEs, and induction of mRNA expression of TNF-alpha, TNF-beta, IL-2 and IFNgamma was observed in PBMC.Furthermore, We detected MF from culture supernatant of Streptococcus anginosus, and partially purified the factor. The MF proliferated to human PBMC,induced production of IL-2 and TNF,and expression of the cytokines mRNA.These results suggest that SPEs or MF from S.pyogens or S.anginosus isolated from oral cavity can be related to periodontitis.