THE RELATIONSHIP BETWEEN BACTERIAL SUPERANTIGEN AND PERIODONTITIS.

细菌超抗原与牙周炎之间的关系。

• 批准号: 05807171
• 负责人: KANEKO Masaru
• 金额: $ 1.15万
• 依托单位: IWATE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807171/
• 关键词: Streptococcus pyogenes; Streptococcus anginosus; Superantigen; Cytokine; Mitogenic factor; Periodontitis; Streptococcal pyrogenic exotoxin; Streptococcal pyrogenic exotoxi

The relationship between biological activities of streptococcal pyrogenic exotoxins (SPE) produced by Streptococcus pyogenes and periodontitis, between biological activities of mitogenic factor (MF) from oral streptococci and periodonttitis were studied. SPEs were purified, and ELISA for detection of these toxins was achieved. These SPEs proliferated to human periferal blood mononuclear cells (PBMC), and promoted to growth T cell bearing TCR Vbeta2. TNF activity and IL-2 production were detected in culture supernatant of PBMC treated with SPEs, and induction of mRNA expression of TNF-alpha, TNF-beta, IL-2 and IFNgamma was observed in PBMC.Furthermore, We detected MF from culture supernatant of Streptococcus anginosus, and partially purified the factor. The MF proliferated to human PBMC,induced production of IL-2 and TNF,and expression of the cytokines mRNA.These results suggest that SPEs or MF from S.pyogens or S.anginosus isolated from oral cavity can be related to periodontitis.

研究了化脓性链球菌产热原性外毒素（SPE）与牙周炎生物活性的关系，口腔链球菌产丝分裂因子（MF）与牙周炎生物活性的关系。对SPEs进行纯化，建立了检测这些毒素的酶联免疫吸附试验。这些spe增殖到人外周血单个核细胞（PBMC），并促进T细胞生长，携带TCR Vbeta2。在SPEs处理的PBMC培养上清液中检测TNF活性和IL-2的产生，并观察到PBMC中TNF- α、TNF- β、IL-2和IFNgamma mRNA表达的诱导。此外，我们还从血管链球菌的培养上清液中检测了MF，并对其进行了部分纯化。MF向人PBMC增殖，诱导IL-2和TNF的产生以及细胞因子mRNA的表达。提示口腔分离的化脓性链球菌或血管性链球菌的SPEs或MF可能与牙周炎有关。

项目成果

Yuko Ohara-Nemoto, Minoru Sasaki, Masaru Kaneko, Takayuki Nemoto and Minoru Ota.: "Cyateine protease activity of streptococcal pyrogenic exotoxin" B.Can.J.Microbiol.40. 930-936 (1994)

Yuko Ohara-Nemoto、Minoru Sasaki、Masaru Kaneko、Takayuki Nemoto 和 Minoru Ota.：“链球菌热原性外毒素的半胱氨酸蛋白酶活性”B.Can.J.Microbiol.40。

佐々木実、根本優子、富樫正幸、金子克: "Streptococcus anginosusが産生するマイトジェニックファクターの部分精製" 岩手医大歯学雑誌. 19. 164-168 (1994)

Minoru Sasaki、Yuko Nemoto、Masayuki Togashi 和 Katsu Kaneko：“咽峡炎链球菌产生的促有丝分裂因子的部分纯化”岩手医科大学牙科杂志 19. 164-168 (1994)。

Y.Ohara-Nemoto,M.Kaneko et al.: "Cysteine protease activity of Streptococcal pyrogenic exotoxin B." Can.J.Microbiol.40. 930-936 (1994)

Y.Ohara-Nemoto、M.Kaneko 等人：“链球菌热原性外毒素 B 的半胱氨酸蛋白酶活性”。

Y.Ohara-Nemeto,M.Kaneko et al.: "Cysteine protease activity of Streptococcal pyrogenic exotoxin B." Can.J.Microbiol. 40. 930-936 (1994)

Y.Ohara-Nemeto、M.Kaneko 等人：“链球菌热原性外毒素 B 的半胱氨酸蛋白酶活性”。

Minoru Sasaki, Yuko Ohara-Nemoto, Masayuki Togashi and Masaru Kaneko.: "Partial purification of soluble mitogenic factor from Streptococcus anginosus culture supernatant." Dent.J.Iwate Med.Univ.19. 164-168 (1994)

Minoru Sasaki、Yuko Ohara-Nemoto、Masayuki Togashi 和 Masaru Kaneko.：“从咽峡炎链球菌培养上清液中部分纯化可溶性有丝分裂因子。”