THE RELATIONSHIP BETWEEN BACTERIAL SUPERANTIGEN AND PERIODONTITIS.

研究了化脓性链球菌产热原外毒素（SPE）的生物学活性与牙周炎的关系，口腔链球菌产促有丝分裂因子（MF）的生物学活性与牙周炎的关系。纯化SPEs，并建立ELISA检测毒素。这些SPE增殖为人外周血单个核细胞（PBMC），并促进携带TCR V β 2的T细胞生长。检测SPEs处理PBMC培养上清中TNF活性和IL-2的产生，观察SPEs诱导PBMC中TNF-α、TNF-β、IL-2和IFN-γ mRNA表达的变化，并检测咽峡炎链球菌培养上清中MF的表达，并对MF进行部分纯化。结果表明，口腔分离的化脓性链球菌或咽峡炎链球菌的SPEs或MF与牙周炎有关。

The relationship between biological activities of streptococcal pyrogenic exotoxins (SPE) produced by Streptococcus pyogenes and periodontitis, between biological activities of mitogenic factor (MF) from oral streptococci and periodonttitis were studied. SPEs were purified, and ELISA for detection of these toxins was achieved. These SPEs proliferated to human periferal blood mononuclear cells (PBMC), and promoted to growth T cell bearing TCR Vbeta2. TNF activity and IL-2 production were detected in culture supernatant of PBMC treated with SPEs, and induction of mRNA expression of TNF-alpha, TNF-beta, IL-2 and IFNgamma was observed in PBMC.Furthermore, We detected MF from culture supernatant of Streptococcus anginosus, and partially purified the factor. The MF proliferated to human PBMC,induced production of IL-2 and TNF,and expression of the cytokines mRNA.These results suggest that SPEs or MF from S.pyogens or S.anginosus isolated from oral cavity can be related to periodontitis.