Structure and Function of the Reaction Center Complexes from Green Sulfur Bacteria

绿硫细菌反应中心复合物的结构和功能

• 批准号: 06640852
• 负责人: SAKURAI Hidehiro
• 金额: $ 1.34万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640852/
• 关键词: Fe-S center; Green sulfur bacteria; Reaction center; Photosynthesis; Chlorobium; Ferredoxin; Cytochrome c; NADP^+ photoreduction; Cyt_c; 光化学反応中心; 緑色硫黄細菌; 光合成電子伝達系; 鉄硫黄センター; シトクロムc; Chlorobiuo tepiduo

Flash-induced optical kinetics at room temperature of cytochrome (Cyt) c_<551> and an Fe-S center (CF_A/CF_B) bound to a purified reaction center (RC) complex from the green sulfur photosynthetic bacterium Chlorobium tepidum were studied. At 551 nm, the flash-induced absorbance change decayd with a t_<1/2> of several hundred ms, and the decay was accelerated by 1-methoxy-5-methylphenazinium methyl sulfate (mPMS). In the blue region, the absorbance change was composed of mPMS-dependent (Cyt) and mPMS-independent component (CF_A/CF_B) which decayd with a t_<1/2> of -400-650 ms. Decay of the latter was effectively accelerated by benzyl viologen (Em -360 mV) and methyl viologen (-440 mV), and less effectively by triquat (-540 mV). The difference spectrum of Cyt c had negative peaks at 551, -520 and -420 nm, with a positive rise a -440 to -500 nm. The difference spectrum of CF_A/CF_B resembled P430 of PSI,and had a broad negative peak at 430-435 nm.Abbreviations : (B) Chl- (bacterio) chlorophyll ; Cyt-cytochrome ; F_A, F_B and F_X-iron-sulfur center A,B and X of photosystem I ; CF_A, CF_B and CF_X-F_A-, F_B- and F_X-like Fe-S center of Chlorobium ; mPMS-1-methoxy-5-methylphenazinium methyl sulfate, PSI-Photosystem I ; RC-reaction center

研究了绿硫光合细菌tepidum的细胞色素（Cyt） c_<551>和Fe-S中心（CF_A/CF_B）与纯化反应中心（RC）配合物结合的室温下闪致光学动力学。在551 nm处，闪致吸光度变化以t_<1/2>衰减数百ms， 1-甲氧基-5-甲基苯肼硫酸甲酯（mPMS）加速衰减。在蓝色区域，吸光度变化由mpms依赖性组分（Cyt）和非mpms依赖性组分（CF_A/CF_B）组成，其衰减时间为-400 ~ 650 ms， t_<1/2>。苯基紫紫素（Em -360 mV）和甲基紫紫素（-440 mV）能有效加速紫紫素的衰变，而三甘醇（-540 mV）对紫紫素的衰变效果较差。Cyt c的差谱在551、-520和-420 nm处呈负峰，在-440 ~ -500 nm处呈正上升。CF_A/CF_B的差谱与PSI的P430相似，在430 ~ 435 nm处有宽的负峰。缩写：(B) Chl-（细菌）叶绿素；Cyt-cytochrome;光系统I的F_A、F_B和f_x -铁硫中心A、B和X；氯藻的CF_A、CF_B和CF_X-F_A-、F_B-和f_x样Fe-S中心；mpms -1-甲氧基-5-甲基苯肼硫酸甲酯；RC-reaction中心

项目成果

Kusumoto, N., Inoue, K., Sakurai, H.: "Spectroscopic studies of bound cytochrome c and an iron-sulfur center in a purified reaction center complex from the green sulfur bacterium Chlorobium tepidum." Photosyn. Res.43. 107-112 (1995)

Kusumoto, N.、Inoue, K.、Sakurai, H.：“来自绿色硫细菌温热绿菌的纯化反应中心复合物中结合细胞色素 c 和铁硫中心的光谱研究。”

Sakurai,H.,Kusumoto,N.,Inoue,K.: "Function of the reaction center of green sulfer bacteria.[invited review]" Photochemistry and Photobiology.

Sakurai，H.，Kusumoto，N.，Inoue，K.：“绿色硫细菌反应中心的功能。[特邀评论]”光化学和光生物学。

Kusumoto,N.: "Spectroscopic studies of bound cytochrome c and an iron-sulfur center in a purified reaction center complex from the green sulfur bacterium Chiorobiuo lepiduo" Photosynth.Res.(印刷中). (1995)

Kusumoto, N.：“来自绿色硫细菌 Chiorobiuo lepiduo 的纯化反应中心复合物中结合细胞色素 c 和铁硫中心的光谱研究”Photosynth.Res（出版中）。

Sakurai, H., Kusumoto, N., Inoue, K.: "Function of the reaction center of green sulfur bacteria. [invited review]" Photochemistry and Photobiology. (in press).

Sakurai, H.、Kusumoto, N.、Inoue, K.：“绿硫细菌反应中心的功能。[特邀评论]”光化学和光生物学。

Kusumoto, N., Inoue, K., Nasu, H., Tomioka, A., Sakurai, H.: "Biochemical and optical studies of photoactive reation center complex form the green sulfur bacterium Chlorobium tepidum." 'photosynthesis : from Light to Biosphere' (ed.P.Mathis) Vol.II,pp.203

Kusumoto, N.、Inoue, K.、Nasu, H.、Tomioka, A.、Sakurai, H.：“绿色硫细菌温热绿菌的光活性反应中心复合物的生化和光学研究。”