Improvement of Photobiological Hydrogen Production of Cyanobacteria by Expressing Different Types of Nitrogenase through Genetic Engineering

为了提高蓝藻基于固氮酶的光生物制氢效率，我们通过基因工程的方法，建立了能优先表达V型或Fe型固氮酶的蓝藻突变体。我们已经敲除了蓝藻的Mo型固氮酶基因，该基因既有Mo型又有V型，或者只有Mo型。我们已经准备了含有纯V或纯Fe类型基因的质粒。为了减少可能从培养玻璃瓶中洗脱出来的钼进入培养基中，我们研制了氢气阻隔塑料袋。

In order to improve the nitrogenase-based photobiological hydrogen production by cyanobacteria, we aimed at creating cyanobacteria mutants that preferentially express V or Fe-only type nitrogenase in place of Mo type, by genetic engineering. We have knocked out the Mo type nitrogenase gene of the cyanobacteria that have either Mo type and V type, or solely Mo type. We have prepared plasmids that harbor V or Fe-only type genes. In order to decrease the Mo possibly eluted from culture glass bottles into the culture medium, we developed hydrogen-gas-barrier plastic bags.