Improvement of Photobiological Hydrogen Production of Cyanobacteria by Expressing Different Types of Nitrogenase through Genetic Engineering

为了提高蓝藻光生物产氢能力，我们通过基因工程的方法，构建了能优先表达V型或fe型氮酶的蓝藻突变体。我们已经敲除了Mo型和V型或只有Mo型的蓝藻的Mo型氮酶基因。我们已经准备了携带纯V型或纯铁型基因的质粒。为了减少从培养玻璃瓶中可能洗脱到培养基中的钼，我们研制了阻氢塑料袋。

In order to improve the nitrogenase-based photobiological hydrogen production by cyanobacteria, we aimed at creating cyanobacteria mutants that preferentially express V or Fe-only type nitrogenase in place of Mo type, by genetic engineering. We have knocked out the Mo type nitrogenase gene of the cyanobacteria that have either Mo type and V type, or solely Mo type. We have prepared plasmids that harbor V or Fe-only type genes. In order to decrease the Mo possibly eluted from culture glass bottles into the culture medium, we developed hydrogen-gas-barrier plastic bags.