Improvement of Photobiological Hydrogen Production of Cyanobacteria by Expressing Different Types of Nitrogenase through Genetic Engineering

为了提高蓝藻基于固氮酶的光生物产氢能力，我们的目标是通过基因工程创建优先表达V或仅Fe型固氮酶而不是Mo型的蓝藻突变体。我们已经敲除了具有Mo型和V型或仅有Mo型的蓝藻的Mo型固氮酶基因。我们已经制备了含有 V 或 Fe-only 型基因的质粒。为了减少Mo可能从培养玻璃瓶中洗脱到培养基中，我们开发了氢气阻隔塑料袋。

In order to improve the nitrogenase-based photobiological hydrogen production by cyanobacteria, we aimed at creating cyanobacteria mutants that preferentially express V or Fe-only type nitrogenase in place of Mo type, by genetic engineering. We have knocked out the Mo type nitrogenase gene of the cyanobacteria that have either Mo type and V type, or solely Mo type. We have prepared plasmids that harbor V or Fe-only type genes. In order to decrease the Mo possibly eluted from culture glass bottles into the culture medium, we developed hydrogen-gas-barrier plastic bags.