Ultrastructure and ontogeny of eyespots in dinoflagellates : an approach from the perspective of their endosymbiotic origins.

甲藻眼斑的超微结构和个体发育：从内共生起源的角度来看的方法。

• 批准号: 06640856
• 负责人: HORIGUCHI Takeo
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640856/
• 关键词: Dimoflagellate; Eyespot; Endosymbiosis; Ultrastructure

Many dinoflagellates, both stigmatic and astigmatic species, were collected from various localities along the Pacific coast and subsequently clonal cultures were established. One of them was found to be a new genus and a species of honyaulacoid dinoflagellates and was described as Amphidiniella sedentaria Horiguchi gen.et sp.nov. Furthermore, a new dinoflagellate, Gymnodinium natalense Horiguchi et Pienaar sp.nov.which possesses a novel type of eyespot was slso described and its ultrastructure and ontogeny of the eyespot was studied in detail.Four species of dinoflagellates were found to contain a chromophyte endosymbiotic alga in this study and these findings made the total number of such dinoflagellates six. These six species share the characteristics, such as similar type of endosymbiont and same type of eyespot (Type B eyespot) which has been found only in these six species among entire dinoflagellates. The ultrastructure of the eyespot of these species was studied. Although these six species are morphologically quite defferent, based on above mentioned shared characteristics, the monophily of these six species was proposed.Since little has been known regarding the ontogeny of eyespot in dinoflagellates, the ontogeny of Type B eyespot was investigated hight microscopically. It was found that the duplication process was mixture of eyespot devision and de novo formation. The presence of organella-DNA in the Type B eyespot was not confirmed.

许多甲藻，柱头和nummatic种，收集了来自不同的地方沿着太平洋沿岸，随后建立克隆文化。本文报道了一个新的甲藻属和一个新的甲藻种，并将其命名为Amphidiniella sedentaria Horiguchigen.et sp. nov.）。此外，还报道了一个新的甲藻种-这6种甲藻的内共生体类型相似，眼斑类型相同（B型眼斑），而这在整个甲藻中是唯一的。本文对这几种昆虫的眼斑进行了超微结构研究。虽然这6种甲藻在形态上有很大差异，但基于上述共同特征，提出了这6种甲藻的单缘性。由于对甲藻眼斑的个体发育知之甚少，本文对B型眼斑的个体发育进行了显微观察。结果表明，复制过程是眼斑分裂和从头形成的混合过程。未证实B型眼斑中存在细胞器DNA。

项目成果

T. Horiguchi: "Ultrastructure of a new marine sand-dwelling dinoflagellate, Gymnodinium quadrilobatum sp. nov (Dinophyceae) with sepcial reference to its endosymbiotic alga." Eur. J. Phycol.29. 237-245 (1994)

T. Horiguchi：“一种新的海洋沙栖甲藻的超微结构，Gymnodinium fourlobatum sp. nov（甲藻纲），特别是其内共生藻类。”

T. Horiguchi: "Amphidiniella sedentaria gen. et sp. nov (Dinophyceae), a new sand-dwelling dinoflagellate from Japan." Phycological Research. 43. 93-99 (1995)

T. Horiguchi：“Amphidiniella sedentaria gen. et sp. nov（甲藻纲），一种来自日本的新沙栖甲藻。”

T. Horiguchi: "Gymnodinium natalense sp. nov. (Dinophyceae), a new tide pool dinoflagellate from South Africa." Jpn. J. Phycol.42. 21-28 (1994)

T. Horiguchi：“Gymnodinium natalense sp. nov.（甲藻纲），一种来自南非的新潮池甲藻。”

T.Horiguchi: "Ultrastructure of a new marine sand-dwelling dinoflagellate,Gymnodinium quadrilobatum sp.nov.(Dinophyceae)with sepcial reference to its endosymbiotic alga." Eur.J.Phycol.29. 237-245 (1994)

T.Horiguchi：“一种新的海洋沙栖甲藻的超微结构，Gymnodinium fourlobatum sp.nov.（甲藻纲），特别是其内共生藻类。”

T. Horiguchi: "Heterocapsa circularisquama sp. nov. (Peridiniales, Dinophyceae) : a new dinoflagellate causing mass mortality of bivalves in Japan." Phycological Research. 43. 129-136 (1995)

T. Horiguchi：“Heterocapsa circularisquama sp. nov.（围甲藻目，甲藻纲）：一种新的甲藻，导致日本双壳类动物大规模死亡。”