Ultrastructure and ontogeny of eyespots in dinoflagellates : an approach from the perspective of their endosymbiotic origins.

许多甲藻，柱头和nummatic种，收集了来自不同的地方沿着太平洋沿岸，随后建立克隆文化。本文报道了一个新的甲藻属和一个新的甲藻种，并将其命名为Amphidiniella sedentaria Horiguchigen.et sp. nov.）。此外，还报道了一个新的甲藻种-这6种甲藻的内共生体类型相似，眼斑类型相同（B型眼斑），而这在整个甲藻中是唯一的。本文对这几种昆虫的眼斑进行了超微结构研究。虽然这6种甲藻在形态上有很大差异，但基于上述共同特征，提出了这6种甲藻的单缘性。由于对甲藻眼斑的个体发育知之甚少，本文对B型眼斑的个体发育进行了显微观察。结果表明，复制过程是眼斑分裂和从头形成的混合过程。未证实B型眼斑中存在细胞器DNA。

Many dinoflagellates, both stigmatic and astigmatic species, were collected from various localities along the Pacific coast and subsequently clonal cultures were established. One of them was found to be a new genus and a species of honyaulacoid dinoflagellates and was described as Amphidiniella sedentaria Horiguchi gen.et sp.nov. Furthermore, a new dinoflagellate, Gymnodinium natalense Horiguchi et Pienaar sp.nov.which possesses a novel type of eyespot was slso described and its ultrastructure and ontogeny of the eyespot was studied in detail.Four species of dinoflagellates were found to contain a chromophyte endosymbiotic alga in this study and these findings made the total number of such dinoflagellates six. These six species share the characteristics, such as similar type of endosymbiont and same type of eyespot (Type B eyespot) which has been found only in these six species among entire dinoflagellates. The ultrastructure of the eyespot of these species was studied. Although these six species are morphologically quite defferent, based on above mentioned shared characteristics, the monophily of these six species was proposed.Since little has been known regarding the ontogeny of eyespot in dinoflagellates, the ontogeny of Type B eyespot was investigated hight microscopically. It was found that the duplication process was mixture of eyespot devision and de novo formation. The presence of organella-DNA in the Type B eyespot was not confirmed.