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Studies on biodiversity and origins of freshwater dinoflagellates.

淡水甲藻的生物多样性和起源研究。

基本信息

批准号：
13640690
负责人：
HORIGUCHI Takeo
金额：
$ 2.18万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640690/
关键词：
Dinoflagellates Freshwater Molecular phylogeny Taxonomy Protista Algae 分子系統解析 18S rDNA

项目摘要

The aims of this project were ; 1)to investigate biodiversity of freshwater dinoflagellate species in Japan, 2)to analyze phylogenetic relationships between these species, using molecular technique, 3)to investigate the origin(s) of freshwater dinoflagellates, and 4)to re-evaluate the present taxonomic system. We have collected number of water samples around Hokkaido and from other area. So far, we have identified some 70 species of dinoflagellates Of these, we have obtained SSU rDNA sequences for about 50 species and have investigated phylogenetic relationships using these sequences. We believe we were able to reveal the biodiversity of freshwater dinoflagellates mainly inhabiting cold-temperate regions.The molecular phylogenetic studies revealed the followings ; 1)the large genera such as Gymnodinium, Gyorodinium, Amphidinium, Peridinium, Peridiniopsis are polyphyletic, 2)Gyrodiniuim can be defined by circular apical groove and longitudinal striae, 3)Cystodinium is probably related to the Woloszynskia, 4)in the Peridinium/Preidiniopsis complex, cyst morphology and surface ornamentation reflect phylogeny, 5)all the species possessing cleptoplastidy are probably monophyletic and 6)Some of the woloszynskoid are related to the symbiotic dinoflagellates, viz. Symbiodiniium.In this project, we have established suitable methods for single-cell PCR and single-cell SEM and these techniques will certainly contribute to further studies and understanding of protist diversity.

本项目的目的是：1）调查日本淡水甲藻物种的生物多样性，2）利用分子技术分析这些物种之间的系统发育关系，3）调查淡水甲藻的起源，以及4）重新评估现有的分类系统。我们在北海道周围和其他地区收集了大量的水样。到目前为止，我们已经鉴定了约70种甲藻，其中，我们已经获得了约50种的SSU rDNA序列，并利用这些序列研究了系统发育关系。我们相信，我们能够揭示主要分布于寒温带的淡水甲藻的生物多样性。1）Gymatinium，Gyorodinium，Amphidinium，Peridinium，Peridiniopsis等大属为复系; 2）Gyrodiniums可由圆形顶沟和纵纹界定; 3）Cystodinium可能与Woloszynskia有关; 4）在多甲藻/拟多甲藻复合体中，孢囊形态和表面纹饰反映了共生性，5）所有具裂生体的种可能都是单系的，6）部分woloszynskoid与共生甲藻共生，即共生甲藻（Symbiodinium）有亲缘关系。我们建立了单细胞PCR和单细胞SEM的方法，这些技术将有助于进一步研究和了解原生生物的多样性。