Genetic mechanism of monocarpic mass flowering and death of Sasa jotanii analyzed by microsatellite DNA markers.

1997年宫岛岛上的一次开花后的苗木种群，再生的秆和未开花的丛生植物。利用水稻微卫星DNA基因座对Oyama进行了大量开花的遗传分析，并与山上三个不育群体进行了比较。宫山，八条岛。分别利用R230、RM205、RM254和RM60四个微卫星座位对A、B、C和D的多基因座进行了分析。共获得50个DNA样本，其中3个来自未开花丛生(UF)，13个再生秆(RC)，8个邻近苗来自RC(VS)，6个非近邻苗来自RC(NN)，4个来自Mt.以长垣(NG)和八路岛(HA)的16株为模板，等位基因A、B、C和D的出现仅限于ISL的种群或秆。Mikurajima，而在八柱岛没有。三个分散在山上的种群。ISL的宫山。Hchijojima的遗传多样性较差，且几乎相同，表明种群起源于老年时的一个连续种群。在Mikurajima人口中，四分之三的NG样本位于距离Mt.Oyama没有与HA群体相似的等位基因。A、B、C基因共现仅见于遗传频率相近的RC和VS，而A在NN中未见。未开花的丛生由6个聚集态的秆组成，其中1个UF只有等位基因A，2个没有，表明该丛由两个以上起源于不同基因的无性系组成。这些结果表明，等位基因A、B和C的组合导致了单性结实的大量开花和死亡，而后者将独立于前者，并由A控制，是致死基因的泄漏。

Seedling populations following the monocarpic mass flowering of Sasa jotanii in the Mikurajima Island in 1997, regenerating culms and unflowered clump on the Mt. Oyama were analyzed on the genetic aspect of the mass flowering using rice microsatellite DNA loci in comparison with three sterile populations on Mt. Miharayama, Hachijojima Island. Four microsatellite loci, R230, RM205, RM254 and RM60 were used for the analyses with respect to the occurrence of multi-locus genes of A, B, C and D, respectively. A total of 50 sample DNAs including three from an unflowered clump (UF), 13 regenerating culms (RC), eight vicinal seedlings to the RC (VS), six non-nearest neighbour seedlings from the RC (NN), four culms from Mt. Nagataki (NG) and 16 from Hachijojima Island (HA) were used as the template.Occurrence of all alleles of A, B, C and D were limited on the populations or culms of Isl. Mikurajima, while none in Hachijojima. Three scattered populations on Mt. Miharayama, Isl. Hachijojima had poor and almost the same genetic diversity suggested that the populations originated from one continuous population in old ages. Among the Mikurajima populations, three out of four samples of NG that located on 1 km south-east from Mt. Oyama had none allelic loci like as the HA populations. Co-occurrence of A, B and C was found only in RC and VS with similar genetic frequencies, whereas A was not found in NN. The unflowered clump composed of six aggregated culms, among which one UF had only allele A, while two had none showed the clump was composed of more than two clones that originated from distinct genets.These results suggests that a combination of genes such as alleles A, B and C induce the monocarpic mass flowering and death, while the latter will be independent from the former and that controlled by A as a leaky of a lethal gene.