The application of lecithin mixed reverse micelles for bioproduction and separation processes

以含β-半乳糖苷酶的胶束体系为基础，研究了反胶束微生物反应器在半乳糖生产中的应用。将β-半乳糖牢固地包封在AOT（琥珀酸二（2-乙基己基）酯磺酸钠）/卵磷脂混合胶束中。添加卵磷脂的AOT反胶束稳定的胶束界面，因此，泄漏的封装的β-半乳糖体的水相是可以忽略不计的，而底物和产品是自由转移和分配，由于它们的低分子量。与常规AOT胶束相比，AOT/卵磷脂混合胶束中增溶的β-gal的稳定性也增加。在本系统中观察到的活性明显高于在本体缓冲溶液中的活性。采用离心分配色谱法（CPC），以包封β-半乳糖的反胶束溶液为固定相，底物水溶液为移动的相，在连续操作模式下进行酶转化和产物回收。CPC接触器的使用消除了对底物和产物的任何传质限制，并且半乳糖在流出物中连续回收。

The application of reverse micellar micro bioreactor systems for enzymatic conversion with continuous recovery of water-soluble products was demonstrated, based on the production of galactose by micelles containing beta-galactosidase. The beta-gal was firmly encapsulated in AOT (bis (2-ethylhexyl) sodium sulfosuccinate) /lecithin mixed micelles. The addition of lecithin to the AOT reverse micelles stabilized the micellar interface, and the leakage of the encapsulated beta-gal to the bulk aqueous phase was therefore negligible, while substrate and product were free to transfer and partition owing to their low molecular weights. The stability of the solubilized beta-gal was also increased in the AOT/lecithin mixed micelles as compared to the conventional AOT micelles. A consider ably higher activity was observed in the present systems than in the bulk buffer solution. Enzymatic conversion and product recovery was carried out in a continuous operation mode by use of centrifugal partition chromatography (CPC), employing the reverse micellar solution with encapsulated beta-gal as the stationary phase and the substrate aqueous solution as the mobile phase. Use of the CPC contactor eliminated any mass transfer limitation on the substrate and the product, and galactose was continuously recovered in the effluent.