Research on Preparationand Application of Stress-Responsive Nano-Colloid

应力响应纳米胶体的制备及应用研究

• 批准号: 08555183
• 负责人: KUBOI Ryoichi
• 金额: $ 4.93万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08555183/
• 关键词: Stress Response; Bio-Separation; Reverse Micelle; Percolation; Refolding; Protein; Hydrophobic Interaction; Liposome; 分離・精製; 分子集合体; 膜透過; 機能材料; 会合コロイド; シャペロニン; 疎水性

The stress responsive functions, for example synthesis of specific proteins including a set of heat shock proteins, refolding of damaged proteins, translocation arid release of proteins across membranes, and formation of protein aggregates in an inert insoluble form etc., of biological nano-colloid such as cells, membranes, and proteins have been quantitatively analyzed by using the percolation method, aqueous two-phase partitioning method, and immobilized liposome chromatographyin the various levels focusing on the transient and weak molecular interactions between proteins and lipid membranes or reverse micelles under various stress conditions. It was found that the formation of a new transient structure between partly denatured proteins and reverse micelles or liposomes played the key role in the above stress resposive functions. It was found to be driven by the fluidity of nano-colloidal interface combined with the transient hydrophobic interaction between them and could be mediated and controlled by kinds of stressors, their combinations, and stress levels. These quantitative results have been successfully utilized for the design and development of novel and efficient protein refolding and bio-separation processes.

应激反应功能，例如包括一组热休克蛋白的特定蛋白质的合成、受损蛋白质的重折叠、蛋白质跨膜的移位和释放以及惰性不溶性形式的蛋白质聚集体的形成等，利用渗滤法、双水相分配法和固定化脂质体色谱法对生物纳米胶体如细胞、膜和蛋白质在不同压力条件下与脂质膜或反胶束之间的瞬时和微弱的分子相互作用进行了定量分析。结果表明，部分变性蛋白质与反胶束或脂质体之间形成的一种新的瞬时结构在上述应激响应功能中起着关键作用。结果表明，纳米胶体界面的流动性和界面间的瞬时疏水作用是纳米胶体降解的驱动力，纳米胶体降解受各种应激源、应激源组合和应激水平的调控。这些定量结果已成功地用于设计和开发新的和有效的蛋白质复性和生物分离过程。

项目成果

Hiroshi Umakoshi, Ryoichi Kuboi, and Isao Komasawa: "Control of Partitioning Behaviors of Bacterial Cells and Characterization of Their Surface Properties in Aqueous Two-Phase Systems" J.Ferment.Bioeng.84 (6). 572-578 (1997)

Hiroshi Umakoshi、Ryoichi Kuboi 和 Isao Komasawa：“水性两相系统中细菌细胞分配行为的控制及其表面性质的表征”J.Ferment.Bioeng.84 (6)。

Dong-Pyo,Hong etal.: "Extraction of Proteins and Polymers Using Reversed Micelles and Percolation Process." Korean J.Chem.Eng.14(5). 334-340 (1997)

Dong-Pyo, Hong 等人：“使用反胶束和渗滤过程提取蛋白质和聚合物。”

Ryoichi Kuboi: "Refolding of Carbonic Anhydrase Assisted by 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Liposomes," Biotechnol.Prog.13. 828-836 (1997)

Ryoichi Kuboi：“1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱脂质体辅助碳酸酐酶的重折叠”，Biotechnol.Prog.13。

Hiroshi Umakoshi, Toshinori Shimanouchi, and Ryoichi Kuboi: "Selective Separation Process of Proteins Based on the Heat Stress-Induced Translocation across Phospholipid Membranes." J.Chromatogr.B. 711. 111-116 (1998)

Hiroshi Umakoshi、Toshinori Shimanouchi 和 Ryoichi Kuboi：“基于热应激诱导的磷脂膜易位的蛋白质选择性分离过程”。

Koichiro Shiomori, Naofumi Ebuchi, Yoshinobu Kawano, Ryoichi Kuboi, and Isao Komasawa: "Extraction Characteristic of Bovine Serum Albumin Using Sodium Bis (2-ethylhexyl) Sulfosuccinate Reverse Micelles" J.Ferment.Bioeng.86 (6). 581-587 (1998)

Koichiro Shiomori、Naofumi Ebuchi、Yoshinobu Kawano、Ryoichi Kuboi 和 Isao Komasawa：“使用双（2-乙基己基）磺基琥珀酸钠反胶束提取牛血清白蛋白的特性”J.Ferment.Bioeng.86 (6)。