Research on Preparationand Application of Stress-Responsive Nano-Colloid

生物纳米胶体如细胞、膜和蛋白质等的应激反应功能，如合成一组热休克蛋白、损伤蛋白质的重折叠、蛋白质跨膜的移位和释放、以及以惰性不溶形式形成蛋白质聚集体等应激反应功能，已被用渗滤法、双水相分配法和固定化脂体层析法在不同水平上定量分析，重点关注在不同应激条件下蛋白质与脂膜或反胶束之间的瞬时和弱分子相互作用。研究发现，部分变性蛋白质与反胶束或脂质体之间形成的一种新的瞬时结构在上述应激反应功能中起着关键作用。研究发现，纳米胶体界面的流动性与它们之间的瞬时疏水相互作用相结合，可以通过不同的应激源、它们的组合和应力水平来调节和控制。这些定量结果已成功地用于设计和开发新的、高效的蛋白质复性和生物分离工艺。

The stress responsive functions, for example synthesis of specific proteins including a set of heat shock proteins, refolding of damaged proteins, translocation arid release of proteins across membranes, and formation of protein aggregates in an inert insoluble form etc., of biological nano-colloid such as cells, membranes, and proteins have been quantitatively analyzed by using the percolation method, aqueous two-phase partitioning method, and immobilized liposome chromatographyin the various levels focusing on the transient and weak molecular interactions between proteins and lipid membranes or reverse micelles under various stress conditions. It was found that the formation of a new transient structure between partly denatured proteins and reverse micelles or liposomes played the key role in the above stress resposive functions. It was found to be driven by the fluidity of nano-colloidal interface combined with the transient hydrophobic interaction between them and could be mediated and controlled by kinds of stressors, their combinations, and stress levels. These quantitative results have been successfully utilized for the design and development of novel and efficient protein refolding and bio-separation processes.