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Development of Separation and Purification Processes with Reverse Micelles Using Functional Reaction

利用功能反应开发反胶束分离和纯化工艺

基本信息

批准号：
63850189
负责人：
KUBOI Ryoichi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1)Reverse Micelle Size Distribution and Mechanism of Protein Solubilization into Reverse Micelles The size and its distribution of reverse micelles prepared by dissolving Aerosol OT in isooctane were measured by using a dynamic light scattering method. These were independent of AOT concentration and were correlated with water content which was controlled by salt concentration in the bulk aqueous phase. A simple scheme for solubilization of proteins into micelle solution has been presented which assumes that the proteins adsorbed on the interface between aqueous and organic solutions may dissolve into the micelles when they encounter with the micelles of which sizes are greater than those of the proteins. The effect of the steric and electrostatic interaction between the micelles and proteins has also been taken into account.Separation of Lysozyme, alpha-Chymotrypsin and BSA was carried out as an application of the present solubilization scheme.2)Separation of Proteins with Reverse Mice … More llar Liquid Membranes Reverse micellar bulk liquid membrane was successfully applied to the separation of proteins with high selectivity by utilizing electrostatic interaction and/or steric interaction(size exclusion effect) between micelles and proteins. Highly selective uphill transport of proteins through the liquid membrane from an aqueous phase to another can be achieved by manipulating pH, concentration of KCl in both aqueous phases and initial water content in the membrane phase and also by selecting anionic or cationic surfactant( AOT or CTAB ). This is effected by the differences in the physical properties of the proteins to be separated such as isoelectric point, surface charge and molecular weight. Denaturation of proteins, mainly caused by complex formation with AOT molecules at low concentration of KCl in the aqueous phase for recovery, is considerably small compared with that encountered in conventional batch extraction. The liquid membrane separation system was further extended to the separation method utilizing the difference of transfer rate of proteins concerned. The combined liquid membrane system with both AOT and CTAB membranes is also effective for the simultaneous separation of proteins. Less

1)反胶团的大小分布及蛋白质增溶到反胶团中的机理通过使用动态光散射法测量通过将Aerosol OT溶解在异辛烷中制备的反胶团的大小及其分布。这些都是独立的AOT浓度和相关的水含量，这是由盐浓度控制的散装水相。本文提出了一个简单的蛋白质增溶于胶束溶液的方案，该方案假定吸附在水溶液和有机溶液界面上的蛋白质，当遇到比蛋白质大的胶束时，可以溶解于胶束中。本文还考虑了胶束与蛋白质之间的空间和静电相互作用的影响，并将其应用于溶菌酶、α-糜蛋白酶和牛血清白蛋白的分离 ...更多信息反胶束本体液膜利用胶束与蛋白质之间的静电相互作用和/或空间相互作用（尺寸排阻效应），成功地应用于蛋白质的高选择性分离。蛋白质通过液膜从一个水相到另一个水相的高选择性上坡转运可以通过控制pH、两个水相中KCl的浓度和膜相中的初始水含量以及通过选择阴离子或阳离子表面活性剂（AOT或CTAB）来实现。这受到待分离蛋白质的物理性质差异的影响，例如等电点、表面电荷和分子量。蛋白质的变性，主要是由复合物的形成与AOT分子在低浓度的KCl在水相中的回收，是相当小的相比，在传统的分批提取中遇到的。将液膜分离系统进一步扩展到利用相关蛋白质转移速率差异的分离方法。AOT膜和CTAB膜的组合液膜系统对于蛋白质的同时分离也是有效的。少