MONITORING DYNAMIC STATE OF WATER IN PLANT TISSUES USING ^1H-NMR

使用^1H-NMR 监测植物组织中水的动态状态

• 批准号: 06660038
• 负责人: IWAYA Mari
• 金额: $ 0.96万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660038/
• 关键词: ^1H-NMR longitudinal relaxation time (T_1); Dynamic state of water; Dwarfism; Chilling response; Tulip (Tulipa gesneriana); Pea (Pisum sativum); Pine wood nematode; プロトンNMR(^1H-NMR); MRI; 環境ストレス; 球根; 縦緩和時間(T_1); 細胞水

^1H-NMR is a non-destructive method that can be used to study the dynamic state of water in vivo. Our studies applying NMR longitudinal relaxation times (T_1) showed that although the state of water in plant tissues is primarily influenced by tissue water content, the dynamic state of cell water estimated by T_1 are not necessarily correlate with the tissues water contents. T_1 values in plants are various in many kinds of species, tissues, and organs. Also, T_1 responds sensitively on different kinds of environmental stresses such as chilling, heat, dehydration and inoculation with nematode. 1) This study focuses on the potential dwarfism in seeedlings of Pisum sativum (cv. Progress No.9) in relation to T_1 reflecting the motional freedom of water protons. 2) The physical state of water and metabolic activity in tulip bulbs held at low temperatures were observed using ^1H-NMR imaging and TNBT staining. A strong free water signal was observed in scales for chilled bulb, while a weak water signal was recorded in non-chilled bulb scales. Signal intensity and NMR relaxation time for tulip bulbs reflected differences in the degree of water binding and metabolic activity between chilled and non-chilled bulbs. 3) Water relations and histological features of pine wood inoculated with pine wood nematode was studied. Thus, it may be pointed out that the awareness of "the dynamic states of water in cells or tissues" is very important for the accurate understanding of all fields of plant physiology.

1H-NMR是一种非破坏性的方法，可用于研究体内水的动态状态。我们应用核磁共振纵向弛豫时间（T_1）的研究表明，虽然植物组织中的水状态主要受组织含水量的影响，但由T_1估计的细胞水动态与组织含水量并不必然相关。植物的T_1值在不同种类、不同组织和不同器官中存在差异。T_1对低温、高温、脱水、线虫接种等环境胁迫反应敏感。1)本研究旨在探讨豌豆幼苗的潜在矮化现象。进展No.9）与反映水质子运动自由度的T_1有关。2)利用~ 1H-NMR成像和TNBT染色技术，对低温下郁金香鳞茎中水分的物理状态和代谢活性进行了观察。一个强的自由水信号中观察到的冷冻灯泡的规模，而弱的水信号记录在非冷冻灯泡规模。郁金香鳞茎的信号强度和NMR弛豫时间反映了冷冻和非冷冻鳞茎之间的水结合度和代谢活性的差异。3)研究了接种松材线虫后松树木材的水分关系和组织学特征。因此，可以指出，认识“水在细胞或组织中的动态”对于准确理解植物生理学的各个领域是非常重要的。

项目成果

井上,眞理ほか5名: "^1H-NMRイメージングによるチューリップ球根の低温充足度の探査" 園芸学会誌別冊. (in press). (1995)

Inoue、Mari 和其他 5 人：“通过 ^1H-NMR 成像探索郁金香球茎的低温充足性”，日本园艺学会杂志（出版中）（1995 年）。

井上、大久保、本岡、石田、狩野、小泉: "^1H-NMRイメージングによるチューリップ球根の低温充足度の探査" 園芸学会雑誌. 64. 508-509 (1995)

Inoue、Okubo、Motooka、Ishida、Kano、Koizumi：“通过 ^1H-NMR 成像探索郁金香球茎的低温充足性”日本园艺学会杂志 64. 508-509 (1995)。

Kaku,S.and M.Iwaya-Inoue: "Environmental stress response in palants studied by ^1H-NMR relaxation time" Crobiology and Cryotechnology. 40. (No.2)16-24 (1994)

Kaku,S. 和 M.Iwaya-Inoue：“通过 ^1H-NMR 弛豫时间研究植物的环境应激反应”微生物学和冷冻技术。

Ikeda, T.and T.Kiyohara.: "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematoda, Bursaphelench xylophilus." J.Expt.Bot.46 (285). 441-449 (1995)

Ikeda, T. 和 T. Kiyohara.：“接种有毒或无毒松材线虫松材线虫的黑松的水关系、木质部栓塞和组织学特征。”

Ikeda,T. and T.Kiyohara: "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematoda, Bursaphelenchus xylophilus." J. Expt. Bot.46. 441-449 (1995)

池田，T.