Construction of chromosomal maps for silkworm, Bombyx mori using genetically mutants

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1. Chromosomal features at different meiotic stage in Bombyx mori were observed using air-dry method. The stage of late pachytene to diplotene in meiotic cell and the early embryonic stage in somatic cell are suitable for characterization of the chromosomes.2. The chromosomal mutant E^<Ds> of Bombyx mori, which was assumed by genetically studies to have attachment or translocation between chromosomes 6 and 7, was investigatelly. This mutant had 27 or 28 chromosomes at metaphase I and 26, 27, 28, 29 and 30 at metaphase II.In addition, the hatchability of egga was so much low. These results indicate that aberration of E^<Ds> is due to a reciprocal translocation between linkage 6 and 7.3. The gene named sol has been known to belong to the 6th linkage group. Three-point experiment involving E and F genes showed that the locus of sol was determined to be -21.0 of the 6th linkage group. We applied fluorescence in situ hybridization for reassessment of this result. Bmantp sequence of Nc gene locus (1.4 on 6th linkage group) was used as a probe. Signals were detected in the middle region of chromosomes, indicating that the gene loci on the 6th linkage group are to be revised.