Chemical mutagenesis

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To develop random mutagenesis system in the silkworm, the possibility of mutagenesis by ethyl nitrosourea (ENU) was investigated. ENU was proved to be a useful chemical source as a result, . The fertilized eggs were immersed in ENU solution. The phenotypes of 798 individuals treated with ENU were evaluated at various stages. Abnormal characters were discovered in 212 individuals of them. To survey the mutant, we selected 40 pairs from sib-crossing of individuals having abnormal characters. In G2 or G3 generation, more than 20 mutants were discovered as mutant candidate. In addition, we developed one method to obtain triploid silkworm with high ratio by using CO2 gas. All of the sperm of triploid silkworm were apyrene sperm that can assist fertilization of eupyrene sperm. Moreover, we performed linkage analysis of mutants with SNPs information and marker gene. We also found new characteristics in wild silkworm, Bombyx mandarina: black pupa and big egg.