Immunocytochemical Localization of Enzymes Involved in Lignification.

参与木质化的酶的免疫细胞化学定位。

• 批准号: 06660212
• 负责人: TAKABE Keiji
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660212/
• 关键词: lignification; immuno-labeling; phenylalanine ammonia lyase; cinnamyl alcohol dehydrogenase; lignin; ヒャクニチソウ; 管状要素

Isolated mesophyl cells of Zinnia elegans L.Canary Bird differentiate into tracheary elements in liqluid medium. These elements develop to have lignified secondary wall thickenings. Using this system, characteristics of two important enzymes, phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD) were investigated by immunological methods.Western blotting analysis indicated that both anti-PAL antiserum and anti-CAD antiserum detected the proteins corresponding to the molecular masses of PAL and CAD in Zinnia.In the differentiating tracheary elements, immuno-labeling of PAL were observed to be dispersed in the cytoplasm and the secondary wall thickenings. They were densely located on the Golgi-derived vesicles and the inner portion of secondary wall thickenings. The localization of CAD was observed to have the same characteristics as PAL.Thus, the enzymes involved in lignification are shown to be localized in lignifying secondary walls.

金丝雀百日（Zinnia elegans L.Canary Bird）离体叶肉细胞在液体培养基中分化为管状细胞。这些元素发展成木质化的二次壁增厚。利用该系统，用免疫学方法研究了苯丙氨酸解氨酶（PAL）和肉桂醇脱氢酶（CAD）两种重要酶的特性。Western blotting分析表明，抗PAL抗血清和抗CAD抗血清均能检测到百日草中PAL和CAD分子质量对应的蛋白。在分化的气管元件中，观察到PAL的免疫标记分散在细胞质和次生壁增厚中。它们密集分布在高尔基衍生囊泡和次级壁增厚的内部。CAD的定位被观察到与pal具有相同的特征，因此，参与木质素化的酶被证明定位于木质素次生壁。

项目成果

HIBINO,Takashi et al.: "Increase of Cinnamaldehyde Groups in Lignin of Transgenic Tobacco Plants Carrying an Antisense Gene for Cinnamyl Alcohol Dehydrogenase" Biosci.Biotech.Biochem. 59. 929-931 (1995)

HIBINO，Takashi 等人：“携带肉桂醇脱氢酶反义基因的转基因烟草植物木质素中肉桂醛基团的增加”Biosci.Biotech.Biochem。

K. Takabe et al.: "Histochemical Observation on Transgenic Tobacco with Autisense CAD gene." Proceedings of 3rd PRWAC. (in press). (1996)

K. Takabe 等人：“带有 Autisense CAD 基因的转基因烟草的组织化学观察”。

高部圭司: "樹木細胞の免疫細胞化学" 組織培養. 21. 209-213 (1995)

Keiji Takabe：“树细胞的免疫细胞化学”组织培养 21. 209-213 (1995)。

K Takabe et al.: "Histochemical Observation on Transgenic Tobacco with Antisense CAD gene." Proceedings of 3rd PRWAC. (in press). (1996)

K Takabe 等人：“带有反义 CAD 基因的转基因烟草的组织化学观察”。

高部 圭司: "樹木細胞の免疫細胞化学" 組織培養. 21. 209-213 (1995)

Keiji Takabe：“树细胞的免疫细胞化学”组织培养 21. 209-213 (1995)。