IMMUNO LABELING OF CALCIUM RELEASE CHANNEL PROTEIN

钙释放通道蛋白的免疫标记

• 批准号: 6119685
• 负责人: TERENCE C WAGENKNECHT
• 金额: $ 0.57万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119685
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical resource; microscopy; minerals; urinary tract

(Supported by NIH AR40615 to T. Wagenknecht) The main objective of our group is to determine the structure of the ryanodine receptor (RyR), the protein assembly that carries out excitation-contraction coupling in skeletal and heart muscle. The main tools we use are cryo-electron microscopy and image processing. We have labeled a ligand to the receptor with nanogold and, if the binding is successful, the high-resolution EM images of the vitrified specimen should show the RyR with the attached probe at a specific location. This ligand, a peptide toxin, has a direct effect on the open/closed state of the channel. The experiments we are carrying out promise to provide new insights into the mechanism by which stimulation of muscle by nerve leads to contraction, an area that is of intense interest to biomedical scientists because of its implications for understanding and treating muscular, including heart, diseases. We determined the optimal imaging and recording conditions for this work on the IVEM, which will be used to supplement the work done with CTEM. Data collection was started, with good results.

(由NIH AR40615至T.Wagenkeck支持)的主要目标 我们的小组将确定兰诺定受体的结构 (RyR)，执行兴奋收缩的蛋白质组装 骨骼肌和心肌的偶联。我们使用的主要工具有 低温电子显微镜和图像处理。我们已经标记了一个 用纳米金与受体配基，如果结合是 成功地，玻璃化标本的高分辨率EM图像 应在特定位置显示连接了探头的RyR。 这种配体是一种多肽毒素，对开放/闭合有直接作用。 通道的状态。我们正在进行的实验有望 为刺激肌肉的机制提供了新的见解 神经导致收缩，这是一个引起人们强烈兴趣的区域 生物医学科学家因为它对理解 以及治疗肌肉疾病，包括心脏疾病。我们确定了 在IVEM上进行这项工作的最佳成像和记录条件， 它将用来补充与反恐专家委员会所做的工作。数据 开始收集，效果良好。