Proteins involved in transport and polymerization of monolignols

在杨树和柏树次生壁形成的早期，在ATP的存在下，松柏糖苷被活跃地运输到液泡中。次生壁形成后期，液泡中的松果素积累量逐渐减少。我们收集了将T-DNA插入到与单酚运输相关的候选基因中的拟南芥种子，并将其培养了10-12周。然后，我们研究了木质素在茎的束间纤维中的分布。其中一个转化子在束间纤维的次生壁中显示出低含量的丁香基木质素。

Coniferin was actively transported into vacuole in the presence of ATP in early stage of secondary wall formation in poplar and Japanese cypress. Coniferin accumulated in vacuole gradually decreased in later stage of secondary wall formation. We collected the Arabidopsis seeds in which T-DNA was inserted to the candidate gene involved in monolignol transportation, and grew them for 10 to 12 weeks. Then we investigated the lignin distribution in interfasicular fibers of stalk. One of the transformants showed low syringyl lignin content in the secondary wall of interfasicular fibers.