BIOLOGICAL ASPECTS OF RESTING EGG FORMATION OF MARINE ROTIFERS AND ITS APPLICATION TO FINFISH HATCHERIES

对不同种群间交配行为的观察表明，双歧杆菌与圆形双歧杆菌之间以及双歧杆菌与SS之间存在生殖隔离。与此相反，圆形双歧杆菌与ss株之间存在交配现象。因此，没有证据表明ss -菌株是一种新的海洋臂尾虫，它很可能被包括在圆尾轮虫中。采用半连续培养方法可以提高静息产蛋效率。有些半连续培养在15-20天由于细菌的影响而崩溃。然而，通过放置过滤垫来去除有机物质来稳定轮虫培养。因此，获得了8.1x10^2个静息卵/天/g（干重）的纳米绿藻收获量，其效率是批量培养的3.0倍。然而，在交换培养水后，轮状双翅虫的休眠卵并没有大量产生。

Observations on mating behavior between different groups indicated that there is reproductive isolation between B.plicatilis and B.rotundiformis and between B.plicatilis and SS strains. In contrast, copulation was observed between B.rotundiformis and SS-strains. Thus, there is no evidence that SS-strains are a new marine Brachionus species, and most likely should be included in B.rotundiformis.The efficiency of resting egg production can be improved by employing the semi-continuous culture method. Some semi-continuous cultures collapsed on 15-20th days due to the bacterial effect. However, the rotifer culture was stabilized by placing filtration mats to remove organic materials. Thus harvest of 8.1x10^2 resting eggs/day/g (dry weight) Nannochloropsis oculata was achieved, which is 3.0 times more efficient than from batch cultures. Resting eggs of B.rotundiformis, however, were not mass produced after the exchange of culture water.