BIOLOGICAL ASPECTS OF RESTING EGG DIAPAUSE AND HATCHING AND ITS APPLICATION TO MASS PRESERVATION TECHNOLOGY

静息蛋滞育和孵化的生物学方面及其在大规模保存技术中的应用

基本信息

  • 批准号:
    08660235
  • 负责人:
  • 金额:
    $ 1.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

Diapause and hatching of the marine rotifer Brachionus plicatilis Muller resting eggs were examined. The total number of nuclei in an embryo during the internal diapause period increased from 22 on Day 2 post spawn to 39 (each n=1) on Day 6. The outer layr of embryo membrane gradually thickens from 1.2 (Day 0) to 4.0 mum (Day 8) (each n=10). Irradiation at more than 350 nm caused 1-25% hatching, but it reached 50-60% at 250-310nm light. The addition of hydrogen peroxide or prostaglandins (E1, E2 or F2 alpha) caused resting egg hatching even in darkness. The production of peroxide in seawater caused by light as well as the oxidation of fatty acid to prostaglandins inside the embryo is a possible mechanism of resting egg hatching.The hatchability of mass produced resting eggs (stored in sea water under the dark at 4゚C) gradually decreased due to bacterial infection of the porous egg surface. The canning method preserves eggs in a dry form, after lyophilization at -40oC.When partial pressures were less than 88 kPa, percent hatching after 6 months ranged between 16 and 20%, similar to that of uncanned eggs (19% hatching), but significantly lower (9 and 11%) at 88 and 101 kpa. Even after 12 months, percent hatching remained at 35% when canning was at 61 kpa. Canned eggs that were treated with NaClO (1ppm) or NFS-Na (5ppm) showed a significantly higher percent hatching (68% and 80%, respectively) than untreated eggs.
研究了海洋轮虫(Brachionus plicatilis Muller)静卵的滞育和孵化情况。在内滞育期间,一个胚胎的细胞核总数从产卵后第2天的22个增加到第6天的39个(每个n=1)。胚膜外层从1.2(第0天)到4.0(第8天)逐渐增厚(每个n=10)。350 nm以上的光照下孵化率为1-25%,250-310nm的光照下孵化率为50-60%。过氧化氢或前列腺素(E1, E2或F2 α)的加入即使在黑暗中也能使休眠蛋孵化。海水在光照作用下产生过氧化物以及脂肪酸在胚胎内氧化为前列腺素可能是静息蛋孵化的机制。大量生产的静息蛋(在4ºC的黑暗海水中保存)由于多孔蛋表面的细菌感染,孵化率逐渐下降。罐装法在-40℃冻干后,以干燥的形式保存鸡蛋。当分压低于88千帕时,6个月后的孵化率在16%至20%之间,与未罐装鸡蛋的孵化率(19%)相似,但在88和101千帕时显着降低(9%和11%)。即使在12个月后,在61kpa的压力下,孵化率仍保持在35%。用NaClO (1ppm)或fs - na (5ppm)处理的罐头鸡蛋的孵化率显著高于未处理的鸡蛋(分别为68%和80%)。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
N.Munuswamy et al.: "Structural differences between the resting eggs of Brachionus plicatilis and Brachionus rotundiformis(Rotifera,Brachionidae):an electron microscopic study" Hydrobiologia. 318. 219-223 (1996)
N.Munuswamy 等人:“褶皱臂尾轮虫和圆形臂尾轮虫(轮虫纲,臂轮科)静息卵之间的结构差异:电子显微镜研究”水生生物学。
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    0
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Balompapueng, M.D.et al.: "Preservation of the euryhaline rotifer(Brachionus plicatilis)resting eggs by canning,method." Hydrobiologia. 358. 163-166 (1997)
Balompapueng,医学博士等人:“通过罐装方法保存广盐轮虫(Brachionus plicatilis)休眠卵。”
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    0
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W.G.Gallardo et al.: "Effect of dimethysulfoxide (dMSO),sodium hydroxide (NaOH),acetone,and ethnol on the population growth,mictic female production,and body size of the rotifer rachionus plicatilis Muller Bull.Fac.Fish.Nagasaki Univ.,No.78(1997)" 長崎大学水産学
W.G.Gallardo 等人:“二甲亚砜 (dMSO)、氢氧化钠 (NaOH)、丙酮和乙醇对褶皱 Rachionus plicatilis 轮虫 Muller Bull.Fac.Fish.Nagasaki Univ 的种群增长、雌性繁殖和体型的影响.,No.78(1997)”长崎大学水产科学
  • DOI:
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  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Balompapueng, M.D., A.Hagiwara, and K.Hirayama: "Preservation of the euryhaline rotifer (Brachionus plicatilis) resting eggs by canning method." Hydrobiologia. 358. 163-166 (1997)
Balompapueng,医学博士、A.Hagiwara 和 K.Hirayama:“通过罐装方法保存广盐轮虫(Brachionus plicatilis)休眠卵。”
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    0
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HAGIWARA Atsushi其他文献

HAGIWARA Atsushi的其他文献

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{{ truncateString('HAGIWARA Atsushi', 18)}}的其他基金

Swimming behavior of zooplankton and its dietary value for fish larvae
浮游动物的游泳行为及其对鱼类幼鱼的膳食价值
  • 批准号:
    17H03862
  • 财政年份:
    2017
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of population dynamics of zooplankton by tagging
通过标记分析浮游动物的种群动态
  • 批准号:
    15K14791
  • 财政年份:
    2015
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Behavioral characteristics of live food zooplanktons: mechanisms involved in environmental responses and their application to fish larval rearing techniques
活食性浮游动物的行为特征:环境响应机制及其在鱼类幼体饲养技术中的应用
  • 批准号:
    24380108
  • 财政年份:
    2012
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Epigenetics of the rotifer Brachionus plicatilis and its use as experimental animal
褶皱臂尾轮虫的表观遗传学及其实验动物应用
  • 批准号:
    24658174
  • 财政年份:
    2012
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Quality changes of fish larvae through manipulation of feeding regimes and their mechanisms.
通过操纵喂养方式及其机制来改变鱼幼体的质量。
  • 批准号:
    21380125
  • 财政年份:
    2009
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regu-ation of bioiogica-funGtbh of marine rotifers and itsappli Gation to breed useful strairms
海洋轮虫生物功能调控及其在有用菌株选育中的应用
  • 批准号:
    18380118
  • 财政年份:
    2005
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the mechanism of mixis induction of marine rotifers and it application to mass production of resting eggs.
海洋轮虫混合诱导机制及其在休眠卵规模化生产中的应用研究
  • 批准号:
    16580153
  • 财政年份:
    2004
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on the biological function of tropical minute rotifers and its application for mass preservation of live food.
热带小轮虫生物学功能及其在活体食品大规模保鲜中的应用研究
  • 批准号:
    14560155
  • 财政年份:
    2002
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
BIOLOGICAL ASPECTS OF RESTING EGG FORMATION, DIAPAUSE AND HATCHING OF SMALL SPECIES ROTIFERS AND ITS APPLICATION TO MASS PRESERVATION TECHNOLOGY
小型轮虫静卵形成、滞育和孵化的生物学特征及其在大规模保存技术中的应用
  • 批准号:
    10660187
  • 财政年份:
    1998
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
BIOLOGICAL ASPECTS OF RESTING EGG FORMATION OF MARINE ROTIFERS AND ITS APPLICATION TO FINFISH HATCHERIES
海洋轮虫静息卵形成的生物学方面及其在有鳍鱼类孵化场的应用
  • 批准号:
    06660245
  • 财政年份:
    1994
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Rotifers as live food for fish larvae: resting egg formation for various rotifer strains
轮虫作为鱼类幼虫的活食:各种轮虫品系的休眠卵形成
  • 批准号:
    20K21340
  • 财政年份:
    2020
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Evolutionary consequences of interspecific hybridization: Daphnia resting egg banks as biological archives
种间杂交的进化后果:水蚤休眠卵库作为生物档案
  • 批准号:
    27051794
  • 财政年份:
    2006
  • 资助金额:
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    Research Grants
BIOLOGICAL ASPECTS OF RESTING EGG FORMATION, DIAPAUSE AND HATCHING OF SMALL SPECIES ROTIFERS AND ITS APPLICATION TO MASS PRESERVATION TECHNOLOGY
小型轮虫静卵形成、滞育和孵化的生物学特征及其在大规模保存技术中的应用
  • 批准号:
    10660187
  • 财政年份:
    1998
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
BIOLOGICAL ASPECTS OF RESTING EGG FORMATION OF MARINE ROTIFERS AND ITS APPLICATION TO FINFISH HATCHERIES
海洋轮虫静息卵形成的生物学方面及其在有鳍鱼类孵化场的应用
  • 批准号:
    06660245
  • 财政年份:
    1994
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
The Ecology of Resting Egg Production in a Marine Calanoid Copepod
海洋巨蜥桡足类静息产卵的生态学
  • 批准号:
    7808857
  • 财政年份:
    1978
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Standard Grant
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