权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of mutual relationship between improvement of plants and experssion of carbon and nitrogen assimilatimg enzymes.

植物改良与碳氮同化酶表达的相互关系分析。

基本信息

批准号：
06660419
负责人：
SASAKAWA Hideo
金额：
$ 1.28万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660419/
关键词：
barley glutamine synthetate isozymes Rubisco varietal variation upper leaves flag leaf grain yield 完全展開葉

项目摘要

Varietal variation of contents of glutamine synthetase isozymes (GS1, GS2), rubisco, solble protein, chlorophyll in barley leaves (upper developed leaves, flag leaves and awns) was analyzed. The results obtained were as follows.(1) The contents of soluble protein, rubisco, GS1, GS2 and chlorophyll in flag leaves and upper developed leaves were obviously decreased in lower leaves of almost all varieties including wild ones. Ratio of GS1 in total GS amount was almost constant or slightly decreased in lower leaves of wild varieties. In contrast, the ratio in almost all cultivars increased. This result strongly suggests that the variation pattern of GS1/GS2 ratio in flag leaves and upper developed leaves characterize the difference of wild varieties and cultivars.(2) The contents of soluble protein and rubisco in awn, flag leaf and upper 1st leaf tended to decrease with aging in many varieties including wild ones. In almost all cultivars, GS isozymes contents decreased with aging of those leaves, while the contents in wild varieties with weedy character increased until 3rd week.(3) There was possitive correlation between rubisco content and grain yield in flag leaf of 2nd and 3rd week and in upper 1st leaf of 2nd week. On the contrary, there was negative correlation between grain yield and GS1 content in flag and upper 1st leaf of 2nd week. These results suggest that grain yield in barley is closely related to the contents of rubisco and GS1 in flag upper 1st leaf.

分析了大麦叶片（上部发达叶、旗叶和芒）中谷氨酰胺合成酶同工酶（GS 1、GS 2）、Rubisco、可溶性蛋白、叶绿素含量的品种间差异。获得的结果如下。(1)旗叶和上部发育叶的可溶性蛋白质、Rubisco、GS 1、GS 2和叶绿素含量在几乎所有品种（包括野生品种）下部叶均明显降低。野生品种下部叶片中GS 1占GS总量的比例基本不变或略有下降。相反，几乎所有品种的比率都增加了。这一结果表明，小麦旗叶和上部叶GS1/GS 2比值的变化规律反映了品种和野生品种的差异。(2)芒、旗叶和上部1叶中可溶性蛋白质和Rubisco的含量在包括野生品种在内的许多品种中均随老化而降低。几乎所有品种的GS同工酶含量均随着叶片衰老而下降，而杂草性野生品种的GS同工酶含量在第3周之前一直上升。(3)第2、3周剑叶和第2周上部1叶Rubisco含量与产量呈显著正相关。相反，籽粒产量与第2周旗叶和上部1叶GS 1含量呈负相关。这些结果表明，大麦籽粒产量与旗上1叶Rubisco和GS 1含量密切相关。