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Environmental stress tolerance in non-leguminous nitrogen fixing plants and symbiont Frankia

非豆科固氮植物和共生植物 Frankia 的环境胁迫耐受性

基本信息

批准号：
11660062
负责人：
SASAKAWA Hideo
金额：
$ 2.24万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Salt tolerance of non-leguminous nitrogen fixing plants (actinorhizal plants) and the symbiont Frankia was investigated. Actinorhizal plants used in this experiment were Casuarina equisetifolia, Elaeagnus macrophylla, Alnus sieboldiana and Alnus hirsuta, which are growing in Japan naturally. One effective Frankia strain was isolated from the root nodules of C. equisetifolia and 3 Frankia strains (Ceql, Emal and Anil) whose host range is different were used in this experiment. The growth of Frankia strains was markedly suppressed by NaCl concentration higher than 100 mM, although the magnitude of salt tolerance considerably differed among strains. However, when the cells depressed their growth by high NaCl concentration were transferred to the medium which did not contain NaCl, they underwent multiplication again. Accordingly, we judged that salt tolerance of Frankia is generally considerably high. Seed germination of 4 actinorhizal plants was markedly inhibited by NaCl and germination did not occur at 200 mM NaCl. These results suggested that sowing of actinorhizal plant seeds is not effective for the recovery of the vegetation in salt-accumulated lands. A. sieboldiana and A. hirsuta seedlings was extremely weak against salt stress. On the other hand, E. macrophylla possessed a pretty high salt tolerance, and C. equisetifolia showed was very strong salt tolerance. The upper limit of Nad concentration for nodulation was 300 mM in C. equisetifolia, 100 mM in E. macrophylla and 50 mM in A. sieboldiana and A. hirsuta.From these results, we concluded that C. equisetifolia-Ceql symbiotic system and E. mocrophrylla-Emal symbiotic system are useful for the recovery of the vegetation in salt-accumulated lands.

研究了非豆科固氮植物（放线根植物）和共生植物法兰克菌的耐盐性。本实验使用的放线根植物为日本天然生长的木麻黄、大叶Elaeagnus、三叶桤木和毛毛桤木。本实验采用三种不同寄主范围的Frankia菌株（Ceql、Emal和Anil），从木叶根瘤中分离出一株有效菌株。NaCl浓度大于100 mM时，Frankia菌株的生长受到明显抑制，但不同菌株的耐盐能力差异较大。然而，当细胞被高浓度NaCl抑制生长时，将其转移到不含NaCl的培养基中，细胞又开始增殖。因此，我们判断法兰克属的耐盐性一般是相当高的。NaCl显著抑制4种放线根植物的种子萌发，在200 mM NaCl处理下不萌发。上述结果表明，放线根植物种子对盐渍地植被恢复效果不明显。青竹和毛竹幼苗对盐胁迫的抵抗能力极弱。另一方面，大叶松具有相当高的耐盐性，木贼叶具有很强的耐盐性。结瘤的Nad浓度上限分别为：木叶松300 mM、大叶松100 mM、木叶松和毛毛松50 mM。结果表明，在盐渍化土地上，阔叶林- ceql共生系统和阔叶林- emal共生系统对植被恢复具有重要作用。