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Studies on stem nodulation and regulatory mechanism of nitrogen fixation in Aeschynomene indica.

籼稻茎结瘤及固氮调控机制研究.

基本信息

批准号：
62560060
负责人：
SASAKAWA Hideo
金额：
$ 1.22万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

1. Antibodies were prepared against nitrogenase and leghemoglobin of soybean nodules to study the reguratory mechanism of dinitrogen fixation in Aeschynomene indica. Nitrogenase of stem and root nodules in A. indica highly crossreacted with the nitrogenase antibody and the precipitin lines completely fused. However, the precipitin lines between Aeschynomene and soybean nodules did not completely fuse. These results suggest that nitrogenases of stem and root nodules in A. indica are highly homologous and are partially homologous with that in soybean nodules. Leghemoglobin antibody prepared against its proteins in soybean nodules did not crossareact well with the nodule extract from A. indica.2. Stem nodulation in A. indica was little affected by light and the supply of higher concentration of nitrogen to the host plants. However, stem nodulation was influenced by temperature and was significantly depressed in 20 C. Stem nodules were successfully formed in the middle portion of the stem, suggesting that stem nodulation is closely related with the development of lenticels.3. Inoculation of rhizobia derived from Sesbania, Arachis, and Mimosa did not form stem nodules in A. indica.

1.为了研究印度合皮木固氮的调节机制，制备了抗大豆根瘤固氮酶和豆血红蛋白的抗体。根瘤固氮酶活性的研究。indica与固氮酶抗体高度交叉反应，沉淀素线完全融合。然而，在Aeschynomene和大豆根瘤之间的沉淀素线没有完全融合。这些结果表明，苜蓿茎瘤和根瘤的固氮酶活性较低。indica与大豆根瘤中的同源性很高，部分同源。用大豆根瘤中的豆血红蛋白蛋白制备的抗体与A. indica.2.茎的形成。光和较高浓度的氮素供应对籼稻的影响不大。但茎的膨大受温度的影响，在20 ℃时，茎膨大受到显著抑制.茎瘤在茎的中部成功形成，表明茎瘤的形成与茎瘤的发育密切相关.接种田菁、花生和含羞草根瘤菌均不能在A. indica。