An analysis of the initial process of skeletal muscle contraction using the optical rotation signal.

使用旋光信号分析骨骼肌收缩的初始过程。

• 批准号: 06670069
• 负责人: WATANABE Akira
• 金额: $ 1.09万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670069/
• 关键词: Muscle; E-C coupling; Contraction; Ryanodine; Nitrendipine; Pertussis Toxin; Optical rotation; 骨格筋; 複屈折性; 施光性; ライアノジン; カフェイン; 旋光性; 横紋筋

The optical rotation signal of a skeletal muscle cell associated with the twitch contraction was recorded from a single frog muscle fiber. For the external medium, 90-95% deuterium oxide Ringer solution was employed to reduce mechanical movement. Moreover, the fiber was lightly pushed to the bottom of the chamber with a cylindrical lens. After these procedures no movement was visible under the dissection microscope. The chamber was placed on the specimen stage of a high-speed polarimeter. The incident light was quasi-monochromatic with a central wavelength of 550nm. When the fiber was stimulated with an electric pulse, a transient increase in dextrorotation with a duration of about 22ms was observed. The polarity of the signal was constant among preparations and did not reverse when the direction of action potential conduction was reversed. Ryanodine reduced the amplitude of the signal to about one-half of the control. Nitrendipine conspicuously reduced the duration of the signal. Pertussis toxin eliminated the optical rotation signal without changing the shape and magnitude of the internally recorded action potential or the birefringence signal recorded separately. We conclude that the optical rotation signal originates from the change in conformation of macromolecules which are related to the process of excitation-contraction coupling.

从单个青蛙肌纤维中记录了与抽搐收缩相关的骨骼肌细胞的旋光信号。对于外部介质，使用90-95%的氧化氘林格溶液以减少机械运动。此外，用圆柱形透镜将光纤轻轻推到腔室的底部。在这些程序之后，在解剖显微镜下没有可见的移动。将该室放置在高速旋光仪的样品台上。入射光为中心波长为550 nm的准单色光。当用电脉冲刺激纤维时，观察到右旋的瞬时增加，持续时间约22 ms。信号的极性是恒定的制剂之间，并没有逆转时，动作电位传导的方向被逆转。Ryanodine将信号的幅度降低到控制的一半左右。尼群地平显著减少信号的持续时间。百日咳毒素消除了旋光信号，而不改变内部记录的动作电位或单独记录的双折射信号的形状和大小。我们认为旋光信号来源于大分子构象的变化，而构象的变化与激发-收缩耦合过程有关。

项目成果

Tsuboi,T.and Watanabe,A.: "Optical rotation and birefringence signals recorded from a single skeletal muscle flbre of a srog." Japanese Journal of Physiology. (発表予定).

Tsuboi, T. 和 Watanabe, A.：“从一只雄性骨骼肌纤维记录的光旋转和双折射信号。”《日本生理学杂志》（即将出版）。

Tsuboi,T.and Watanabe,A.: "Optical rotation signals recorded from an isolated single skeletal muscle fibre of a frog." Japanese Journal of Physiology. (発表予定).

Tsuboi, T. 和 Watanabe, A.：“从青蛙的孤立的单个骨骼肌纤维记录的光旋转信号。”日本生理学杂志（即将出版）。

Tsuboi, T.and Watababe, A: "Optical rotation signals recorded from a single skeletal muscle fiber of a frog." Paper. (submitted).

Tsuboi, T. 和 Watababe, A：“从青蛙的单个骨骼肌纤维记录的光旋转信号。”

Tsuboi,T.and Watanabe,A.: "Optical rotation signals recorded from a single skeletal muscle fibre of a frog." Paper submitted. (発表予定).

Tsuboi, T. 和 Watanabe, A.：“从青蛙的单个骨骼肌纤维记录的光学旋转信号。”提交的论文。

Tsuboi, T.and Watanabe, A: "Optical rotation and birefringence signals recorded from a single skeletal muscle fiber of a frog." Japanese Journal of Physiology. (in the press).

Tsuboi, T. 和 Watanabe, A：“从青蛙的单个骨骼肌纤维记录的光旋转和双折射信号。”