Investigation for roles of prostaglandins in the cerebral facilitatory mechanisms of antidiuretic hormone secretion

前列腺素在抗利尿激素分泌脑促进机制中的作用研究

• 批准号: 06670076
• 负责人: YAMAGUCHI Kenichi
• 金额: $ 1.41万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670076/
• 关键词: osmotic stimulus; osmoreceptors; anteroventral 3rd ventricular region; antidiuretic hormone; vasopressin,; prostaglandins; meclofenamate; バソプレッシン; 浸透圧刺激(受容器); 弓状核; カテコールアミン

1.In conscious rats iv infusion of hypertonic saline enhanced plasma antidiuretic hormone (ADH) 15 and 30 min later, and this was accompanied by an augmentation of plasma osmolality, sodium and chloride. The ADH response was abolished by local injection of meclofenamate, an inhibitor of prostaglandin biosynthesis, into the anteroventral third ventricular region (AV3V) 30 minbefore the onset of the hypertonic saline infusion. Factors other than plasma ADH were not affected by the meclofenamate injection significantly. The injection sites were confirmed histologically to have been in or near the organum vasculosum of the laminae terminalis, medial preoptic area, and periventricular and median preoptic nuclei. Local applications of PGE2 into such areas caused, 5 and 15 min later, remarkable increases in plasma ADH and arterial pressure, without affecting plasma osmolality and the electrolytes . These effects of PGE2 and meclofenamate were not observed when injection cnnulae were incidenta … More lly deviated from those AV3V sites to the cerebral ventricle near by, or when intended applications into the ventricle were performed. Therefore, it seems unlikely that the drugs administered into the AV3V produced the effects by acting on regions remote from it via diffusion through a stream of cerebrospinal fluid. On the other hand, AV3V injections of meclofenamate were without effect on plasma ADH and the other factors during iv infusion of isotonic saline.2.Effects of hyperosmolality on endogenous PGE2 levels were investigated, by establishing its determination methods by radioimmunoassay. Rats were infused iv with isotonic or hypertonic saline, and were decapitated for bloodcollection at 0,15 and 30 min. Plasma PGE2 level was not affected significantly by either isotonic or hypertonic saline. However, PGE2 concentration in the interstitial fluid obtained through microdialysis probes implanted into the AV3V one day before experiments tended to increase during the first 30 min period of hypertonic saline infusion performed over 60 min.The results mentioned above suggest that Pgs generated in and/or near the AV3V may contribute to facilitation of ADH secretion in the hyperosmotic state. Less

1.清醒大鼠静注高渗盐水15分钟和30分钟后，血浆抗利尿激素（ADH）升高，并伴有血浆渗透压、钠和氯的升高。在高渗盐水灌注开始前30 min，将前列腺素生物合成抑制剂甲氨蝶呤局部注射到第三脑室前腹侧区（AV 3V），可消除ADH反应。除血浆ADH外，其他因素均未受到注射甲氨蝶呤的显著影响。经组织学证实，注射部位位于终板血管器、内侧视前区以及室周和内侧视前核内或附近。局部应用PGE 2到这些地区造成，5和15分钟后，血浆ADH和动脉压显着增加，而不影响血浆渗透压和电解质。当注射Nulae时，PGE 2和Meclampamate没有观察到这些作用。 ...更多信息 在进行心室内的预期应用时，从这些AV 3V部位偏离到脑室附近。因此，给药至AV 3V的药物似乎不太可能通过脑脊液流扩散作用于远离AV 3V的区域而产生效应。AV 3V注射液对静注等渗盐水时血浆ADH及其他因子无影响。2.建立了高渗对内源性PGE 2的放射免疫测定方法，探讨了高渗对内源性PGE 2的影响。等渗或高渗生理盐水静脉注射后，分别于0、15、30 min断头取血，血浆PGE 2水平无明显变化。然而，通过微透析探针植入到AV 3V实验前一天获得的间质液中的PGE 2浓度往往增加在第一个30分钟期间的高渗盐水输注进行超过60分钟。上述结果表明，PGS产生的和/或附近的AV 3V可能有助于促进ADH分泌的高渗状态。少

项目成果

山口賢一: "6-hydroxydopamine (6-OHDA)の弓状核内投与による浸透圧性vasopressin分泌の増強" 日本内分泌学会雑誌. 70. 211 (1994)

Kenichi Yamaguchi：“通过弓形内施用 6-羟基多巴胺 (6-OHDA) 增强渗透性加压素分泌”，日本内分泌学会杂志 70. 211 (1994)。

Ken'ichi Yamaguchi: "Effects of corticosteroids on cytosolic free calcium concentration of the rat adrenal zona glomerulosa cell." Biological Signals. 2. 352-358 (1994)

Kenichi Yamaguchi：“皮质类固醇对大鼠肾上腺肾小球带细胞胞质游离钙浓度的影响。”

Ken'ichi Yamaguchi: "A search for cerebral neurotransmitters involved in osmoregulation of vasopressin release." Abstracts of the Second Joynt Meeting of the Physiological Societies of Japan,United Kingdom and Eire.161 (1995)

Kenichi Yamaguchi：“寻找参与加压素释放渗透压调节的脑神经递质。”

Yamaguchi, K., Hama, H.and Watanabe, K.: "Roles of prostaglandins generated in the anteroventral third ventricular region in the hyperosmolality-evoked vasopressin secretion in conscious rats." (submitted.).

Yamaguchi, K.、Hama, H. 和 Watanabe, K.：“意识大鼠前腹侧第三脑室区域产生的前列腺素在高渗透压诱发的加压素分泌中的作用。”

Ken′ichi Yamaguchi: "Roles of catecholaminergic neurons located in the arcuate hypothalamic nucleus in the osmotic regulation of vasopressin release." Japanese Journal of Physiology. 44Suppl.I. S270- (1994)

Kenichi Yamaguchi：“位于下丘脑弓形核中的儿茶酚胺能神经元在加压素释放的渗透调节中的作用。”44Suppl.I。