INCREASED Na CONCENTRATION IN CSF IS INVOLVED IN MAINTAINING ARTERIAL PRESSURE IN DEHYDRATED RATS.

脑脊液中 Na 浓度的增加涉及维持脱水大鼠的动脉压。

• 批准号: 05670073
• 负责人: NOSE Hiroshi
• 金额: $ 1.41万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670073/
• 关键词: dehydration; rehydration; osmorecptors; blood pressure; cerebrospinal fluid; baroreflexes; Osmoreceptors; Arterial pressure; Dehydration; Hypovolemia

To analyze the mechanism of the greater decrease in mean arterial pressure (MAP) at a given decrease in [Na]csf (DELTAMAP/DELTA[Na]csf) in dehydrated rats, we measured [Na]csf with a Na sensitive microelectrode in the lateral ventricle (LV) and MAP during infusion (INF) of hypertonic arterial cerebrospinal fluid (ACSF) (-400 mOsm) into the LV of rats with and without sino-aortic denervation (SAD). [Na]csf in both groups increased significantly -1min after the start of INF and attained the maximal increases of 16.4<plus-minus>2.4 meq/kg H_2O in innervated rats and 20.5<plus-minus>1.3 meq/kg H_2 in SAD rats (n=10) at the end of INF.There was no significant difference in DELTA[Na]csf between the two groups throughout the experiment. On the other hand, MAP in SAD rats increased to a significantly higher level than in innervated rats from 6.0 to 17.0 min after the start of INF (p<0.05) and the increase at the end of INF was 19.0<plus-minus>2.3 mmHg and 7.7<plus-minus>1.4 mmHg in the SAD and innervated rats, respectively (p<0.01). Changes in MAP in both groups were highly correlated with DELTA[Na]csf throughout the experiment, but the slope was steeper by two-folds in SAD rats (r=0.97, p<0.001) than that in innervated rats (r=0.97, p<0.001) and was identical to that in dehydrated rats (r=0.96, p<0.001).To cralifiy the mechanism of the decrease in MAP during hypotonic INF into the LV of dehydrated rats, [Na]csf, MAP,CVP (mmHg), and cardiac output (CO,ml/min 100g) were measured in dehydrated rats when [Na]csf was gradually reduced to the pre-dehydrated level. Total vascular conductance (TVC, (ml/min 100g) /mmHg) was calculated as TVC=CO/ (MAP-CVP). MAP and CO decreased and TVC increased as [Na]csf decreased with high correlations (r^2=0.94-0.96, p<0.001).The increase in [Na]csf is important to maintain arterial pressure by causing systemic vasoconstriction and preventing the decrease in CO during dehydration.

为了分析脱水大鼠中在[Na]csf（DELTAMAP/DELTA[Na]csf）给定降低时平均动脉压（MAP）更大降低的机制，我们在将高渗动脉脑脊液（ACSF）（~ 400 mOsm）输注（INF）到具有和不具有窦-主动脉去神经（SAD）的大鼠的侧脑室（LV）期间用Na敏感微电极测量[Na]csf和MAP。两组大鼠的[Na]csf在INF开始后-1min均显著升高，INF结束时达最大值，神经支配组为16.4 ± <plus-minus>2.4meq/kgH_2O，<plus-minus>SAD组为20.5 ± 1.3meq/kgH_2。SAD组MAP在INF开始后6.0 ~ 17.0min显著高于神经支配组（p&lt;0.05），INF结束时SAD组和神经支配组MAP分别增加19.0 ± <plus-minus>2.3mmHg和7.7 ± <plus-minus>1.4mmHg（p&lt;0.01）。在整个实验过程中，两组的MAP变化与DELTA[Na]csf高度相关，但SAD大鼠的斜率要陡两倍（r=0.97，P&lt;0.001）（r=0.97，P&lt;0.001），与脱水大鼠相同（r=0.96，p&lt;0.001）.为探讨低渗INF对脱水大鼠左室MAP降低的机制，测定了[Na]CSF、MAP、CVP（mmHg）和心输出量当[Na]CSF逐渐降低至脱水前水平时，测定脱水大鼠的CO（ml/min 100 g）。总血管传导率（TVC，（ml/min 100 g）/mmHg）计算为TVC=CO/（MAP-CVP）。随着[Na]csf的降低，MAP和CO降低，TVC增加，具有高度相关性（r^2=0.94-0.96，p&lt;0.001）。[Na]csf的增加通过引起全身血管收缩和防止脱水期间CO的降低而对维持动脉压很重要。

项目成果

H.Nose et al.: "Right atrial pressure and ANP release during prolonged exercise in a hot environment." J.Appl.Physiol.(in press). (1994)

H.Nose 等人：“在炎热环境中长时间运动时的右心房压力和 ANP 释放。”

Nose,H.,et al.: "Right atrial pressure and forearm blood flow during prolonged exercise in a hot environment" Pfluegers Archiv.426. 177-182 (1994)

Nose,H.,et al.：“在炎热环境下长时间运动时的右心房压力和前臂血流量”Pfluegers Archiv.426。

Nose,H.et al.: "Shift in body fluid compartments after dehydration in humans. In:Fluid replacement and heat stress." Natinal Academy Press., 15 (1993)

Nose,H.等人：“人体脱水后体液室发生变化。见：液体补充和热应激。”

Nose,H.,et al.: "Right atrial pressure and ANP release during prolonged exercise in a hot environment" J.Applied Physiology. 76. 1882-1887 (1994)

Nose,H.,et al.：“在炎热环境中长时间运动时的右心房压力和 ANP 释放”J.Applied Physiology。

Mack,B.W.et al.: "Influence of exercise intensity and plasma volume on active cutaneous vasodilation in humans." Med.Sci.Sports Exerc.26. 209-216 (1994)

Mack,B.W.等人：“运动强度和血浆量对人类主动皮肤血管舒张的影响。”