Evaluation for functional correlation between prostaglandins and dopamine in regulation mechanisms of ADH release in the AV3V

AV3V 中 ADH 释放调节机制中前列腺素和多巴胺之间功能相关性的评估

• 批准号: 08670076
• 负责人: YAMAGUCHI Kenichi
• 金额: $ 1.22万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670076/
• 关键词: anteroventral 3rd ventricular; prostaglandins; dopamine; catecholamines; antidiuretic hormone; cardiovascular system; hemorrhage; hypovolemia; バゾブレッシン; メクロフェナメイト

1. The aims of reseach project were to examine roles of prostaglandins (PGs) synthesized in the anteroventral third ventricular region (AV3V) in regulation mechanisms of ADH release and cardiovascular system under hypovolemic condition, and to evaluate those of AV3V catecholamine receptors in mediating responses to PGE2. Experiments were conducted in conscious rats with cerebral and vascular cannulae implanted chronically.2. Blood of 1% of body weight was removed twice (H1 and H2) from the femoral artery. H1 increased plasma ADH (pADH) without affecting arterial pressure (AP) and heart rate (HR), whereas after H2 further augmentation of pADH were produced with conspicuous falls in AP and rises in plasma angiotensin II (ANGII). These homorrhages did not alter plasma osmolality or sodium, but increased plasma chloride and decreased plasma potassium.3. An inhibitor of PG synthesis meclofenamate (MCL) infused locally into the AV3V prevented the pADH responses to the bleeding, potentiated t … More hose of AP, and caused singnificant reduction of HR following H2. Other variable such as plasma ANGII were not influenced by MCL.These effects of MCL were not caused when it was infused into the nucleus accumbens, a region sligtlhy distant from the AV3V,or into the cerebral ventricle.4. When PGE2 was applied into the AV3V sites near infusion points of MCL,pADH,AP and HR increased singnificantly.5. Local infusion of dopamine (DA) and an alpha-adrenegic agonist phenylephrines (PHE) into the AV3V elicited transient augmentation of pADH.DA influenced none of the other variables, whereas PHE application caused concomitant reises of AP in approximately 50% of tests. The AV3V infusion of a beta-adrenergic agonist isoproterenol induced depressor and tachycardiac effects.6. The effects of PHE on both the pADH and AP were abolished by prior application of an alpha-adrenergic antagonist phenoxybenzamine into the AV3V.The effect of DA on pADH was blocked by the prior administration of a D1-DAergic antagonist SCH23390, but not by that of a D2-DAergic antagonist sulpiride. The application of these antagonists alone did not affect any of the variables including pADH.7. The AV3V infusion of phenoxybenzamine, SCH23390 and sulpiride were without significant effect on the PGE2-evoed responses of pADH,AP and HR.8. These results suggest that PGs formed in the AV3V may play important roles in regulating ADH release, AP and HR,and that the effects of PGE2 on these factors may not be mediated by AV3V catecholamine receptors, despite their ability to influence the factors. Less

1.本研究旨在探讨第三脑室前腹侧区（AV 3V）合成的前列腺素（PGs）在低血容量状态下ADH释放和心血管系统调节机制中的作用，以及AV 3V儿茶酚胺受体在介导PGE 2反应中的作用。实验采用清醒大鼠，长期植入脑血管插管.从股动脉中取出1%体重的血液两次（H1和H2）。H1增加血浆ADH（pADH），而不影响动脉压（AP）和心率（HR），而H2后，进一步增加pADH，AP明显福尔斯，血浆血管紧张素II（ANGII）上升。这些出血并不改变血浆渗透压或钠，但增加血浆氯和降低血浆钾。将PG合成抑制剂Meclampamate（MCL）局部注入AV 3V可阻止pADH对出血的反应，增强pADH对出血的反应，并可抑制pADH对出血的反应。 ...更多信息 H2后HR明显降低。MCL对血浆ANGII等指标无明显影响，但MCL注入离AV 3V稍远的丘脑背核或脑室后，不会引起上述效应.当PGE 2应用于MCL输注点附近的AV 3V部位时，pADH、AP和HR均显著升高.局部注入多巴胺（DA）和α-肾上腺素能激动剂苯肾上腺素（PHE）到AV 3V引起短暂的增强pADH。DA不影响其他变量，而PHE的应用引起AP在大约50%的测试伴随上升。AV 3V输注β-肾上腺素能激动剂异丙肾上腺素诱导降压和心动过速效应。PHE对pADH和AP的作用可被预先应用α-肾上腺素能拮抗剂酚苄明阻断。DA对pADH的作用可被预先应用D1-DA能拮抗剂SCH 23390阻断，但不能被D2-DA能拮抗剂舒必利阻断。单独应用这些拮抗剂不影响任何变量，包括pADH。AV 3V输注酚苄明、SCH 23390和舒必利对PGE 2诱发的pADH、AP和HR反应无显著影响。这些结果表明，在AV 3V中形成的PG可能在调节ADH释放，AP和HR中起重要作用，并且PGE 2对这些因子的作用可能不是由AV 3V儿茶酚胺受体介导的，尽管它们能够影响这些因子。少

项目成果

Ken'ichi Yamaguchi, Hitoshi Hama and Kazuo Watanabe: "Possible roles of prostaglandins in the anteroventral third ventricurelar region in the hyperosmolality-evoked vasopressin secretion of conscious rats." Experimental Brain Research. 113. 265-272 (1997)

Kenichi Yamaguchi、Hitoshi Hama 和 Kazuo Watanabe：“第三脑室前腹区前列腺素在清醒大鼠高渗透压诱发的加压素分泌中的可能作用。”

Ken'ichi Yamaguchi: "Regulation of AVP release and cardiovascular function by brain prostaglandins synthesized in the AV3V" Neuroscience Research. Suppl.21. s272 (1997)

Kenichi Yamaguchi：“AV3V 中合成的大脑前列腺素调节 AVP 释放和心血管功能”神经科学研究。

Ken'ichi Yamaguchi, Hitoshi Hama and Kanemitsu Yamaya: "Roles of prostaglandins synthesized in the AV3V in the osmotic vasopressin secretion." Folia Endocrinologica Japonica. 72. 303 (1996)

Kenichi Yamaguchi、Hitoshi Hama 和 Kanemitsu Yamaya：“AV3V 中合成的前列腺素在渗透性加压素分泌中的作用。”

Ken'ichi Yamaguchi: "Prostaglandins produced in the AV3V may participate in hyperosmolality-evoked vasopressin secretion." Japanese J.Physiology. 46 Suppl.s189- (1996)

Kenichi Yamaguchi：“AV3V 中产生的前列腺素可能参与高渗透压诱发的加压素分泌。”

Ken'ichi Yamaguchi: "Possible roles of prostaglandins in the anteroventral third ventricular region in the hyperosmolality-evoked vasopressin secretion in conscious rats." Experimental Brain Research. 113. 265-272 (1997)

Kenichi Yamaguchi：“意识大鼠前腹侧第三脑室区域前列腺素在高渗透压诱发的加压素分泌中的可能作用。”