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Elucidation of the proliferative mechanism of fat cells : Abasic study of the obesity

阐明脂肪细胞的增殖机制：肥胖症的基础研究

基本信息

批准号：
06670230
负责人：
SUGIHARA Hajime
金额：
$ 1.34万
依托单位：
Saga Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670230/
关键词：
Fat Tissue Fat Cells Proliferation Small Fat Cells Extracellular Matrix Differentiation Obesity Adult Disease

项目摘要

The proliferative ability of fat cells has been emphasized, both in the fat tissue in vivo and in the culture. We studied the proliferation of unilocular fat cells of young rats in a three-dimensional collagen gel matrix culture, which provides a physiological environment for fat cells. In this setting, the unilocular fat cells were able to maintain their cellular function and actively proliferate. In the culture of unilocular fat cells derived from 3 to 6-week-old rats in this study, very small fat cells appeared at the surface of a unilocular fat cell after the division of the nucleus. Thus, the small fat cells were born mostly in a "budding" manner and then proliferated. Insulin accelerated this process. In other unilocular fat cells, lipid droplets divided after the division of the nucleus, and very small fat cells appeared inside the unilocular fat cells. This was done in a "sharing" manner. Adenosine deaminase, a potent lipolytic factor, accelerated this process. An analysis of the cellular volume by a Coulter counter confirmed the birth of small fat cells from the unilocular fat cells. These small fat cells enlarged and turned into unilocular fat cells. It is supposed that not all unilocular fat cells are terminally differentiated, and do not lose their proliferative activity.

无论是在体内脂肪组织中还是在培养物中，脂肪细胞的增殖能力都得到了强调。我们研究了年轻大鼠单房脂肪细胞在三维胶原凝胶基质培养中的增殖，该基质为脂肪细胞提供了生理环境。在这种情况下，单室脂肪细胞能够维持其细胞功能并积极增殖。在本研究中，在来自3至6周龄大鼠的单室脂肪细胞的培养物中，在细胞核分裂后，在单室脂肪细胞的表面出现非常小的脂肪细胞。因此，小脂肪细胞大多以“出芽”方式出生，然后增殖。胰岛素加速了这一过程。在其他单室脂肪细胞中，细胞核分裂后脂滴分裂，在单室脂肪细胞内出现非常小的脂肪细胞。这是一种“分享”的方式。腺苷脱氨酶，一种强有力的脂肪分解因子，加速了这一过程。通过Coulter计数器对细胞体积的分析证实了小脂肪细胞从单室脂肪细胞中产生。这些小的脂肪细胞变大并变成单室脂肪细胞。据推测，并非所有的单室脂肪细胞都是终末分化的，并且不会失去其增殖活性。