Elucidation of the proliferative mechanism of fat cells : Abasic study of the obesity

脂肪细胞的增殖能力已被强调，无论是在体内的脂肪组织和在培养。我们研究了幼鼠单眼脂肪细胞在三维胶原凝胶基质培养中的增殖情况，为脂肪细胞的生长提供了生理环境。在这种情况下，单眼脂肪细胞能够维持其细胞功能并积极增殖。本研究对3 ~ 6周龄大鼠的单眼脂肪细胞进行培养，单核分裂后，单眼脂肪细胞表面出现了非常小的脂肪细胞。因此，小脂肪细胞大多以“萌芽”的方式诞生，然后增殖。胰岛素加速了这个过程。在其他单室脂肪细胞中，脂滴在细胞核分裂后分裂，在单室脂肪细胞内出现非常小的脂肪细胞。这是以“共享”的方式完成的。腺苷脱氨酶，一种有效的脂溶因子，加速了这一过程。库尔特计数器对细胞体积的分析证实了小脂肪细胞是从单眼脂肪细胞中产生的。这些小脂肪细胞扩大并变成单眼脂肪细胞。据推测，并非所有的单眼脂肪细胞都是终末分化的，并且不会失去它们的增殖活性。

The proliferative ability of fat cells has been emphasized, both in the fat tissue in vivo and in the culture. We studied the proliferation of unilocular fat cells of young rats in a three-dimensional collagen gel matrix culture, which provides a physiological environment for fat cells. In this setting, the unilocular fat cells were able to maintain their cellular function and actively proliferate. In the culture of unilocular fat cells derived from 3 to 6-week-old rats in this study, very small fat cells appeared at the surface of a unilocular fat cell after the division of the nucleus. Thus, the small fat cells were born mostly in a "budding" manner and then proliferated. Insulin accelerated this process. In other unilocular fat cells, lipid droplets divided after the division of the nucleus, and very small fat cells appeared inside the unilocular fat cells. This was done in a "sharing" manner. Adenosine deaminase, a potent lipolytic factor, accelerated this process. An analysis of the cellular volume by a Coulter counter confirmed the birth of small fat cells from the unilocular fat cells. These small fat cells enlarged and turned into unilocular fat cells. It is supposed that not all unilocular fat cells are terminally differentiated, and do not lose their proliferative activity.