Cell Biological Study of "Adipocytes Growth Factor" in fat cells of the obese in tissue culture.

脂肪细胞培养体系的建立是开展肥胖人群脂肪细胞生物学研究的前提。在培养基中培养时，成熟的白色脂肪细胞不会附着在培养皿的底表面。相反，由于它们的高脂含量，它们倾向于漂浮在培养基的顶部。我们成功地建立了一种培养成熟脂肪组织碎片的新方法。来源于脂肪组织的成纤维细胞样脂肪细胞大量增生。用这种培养方法对脂肪细胞生长因子进行了研究。结果表明，在培养条件下，脂肪细胞、胰岛素和愈伤原的条件培养基加速了细胞的增殖。从条件培养基中提取一些物质作为“脂肪细胞生长因子”进行分析。胰岛素的作用是非特异性的。胶原蛋白是脂肪细胞必需的细胞外基质。胶原蛋白的作用被认为是合理的脂肪细胞的增殖和分化。

The establishment of a culture system of fat cells is a prerequisite for study of the cell biology of fat cells of the obese.Mature white fat cells do not attach themselves to the bottom surface of a culture dish when incubated in medium. Instead, they tend to float on top of the medium because of their high lipid content. We succeeded a new method for culturing mature fat tissue fragments. Fibroblast-like fat cells derived from the fat tissue proliferated extensively. "Adipocytes Growth Factor" was studied with this culture method.As the result, the conditioned medium of the fat cells, insulin and callagen accelerated the proliferation in the culture condition. Some substance shall be analyzed as the "Adipocytes Growth Factor" from the conditioned medium. The effect of insulin is nonspecific. Collagen is a essential extracellular matrix for the fat cells. The effect of collagen is thought to be reasonable to the proliferation and differentiation of the fat cells.