Extinction of TCR/CD3 and lck gene expression in somatic cell hybrids.

体细胞杂交体中 TCR/CD3 和 lck 基因表达的消失。

• 批准号: 06670245
• 负责人: OIKAWA Tsuneyuki
• 金额: $ 1.34万
• 依托单位: Sasaki Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670245/
• 关键词: gene experssion; extinction; hybrid; T cell; TCR; lck; lckがん遺伝子; T細胞抗原受容体遺伝子; 細胞融合

The regulation of expression of cell type-specific genes during cell differentiation is a complex process involving interaction between multiple positive and negative reguratory factors. The somatic cell hybrid system has been used to study hierarchial negative regulation of cell type-specific genes.We found that expression of the TCR/CD3 and lck genes is extinguished in T cell x fibroblast cell hybrids. In the hybrids, expression of the genes encoding lymphoid-specific transcription factors, such as LEF-1, TCF-1, GATA-3, Ikaros/LyF-1, Elf-1, Myb and Fli-1, is extinguished, while that of the genes encoding ubiquitously expressed transcription factors, such as Sp-1, Ets-2 and CREB,is not extinguished.These results suggest that putative repressors in fibroblast shut off the expression of several lymphoid-specific transcription factors and consequently downregulate T cell specific structural gene expression.

细胞分化过程中细胞类型特异性基因表达的调节是一个复杂的过程，涉及多种正调节因子和负调节因子之间的相互作用。体细胞杂交系统已用于研究细胞类型特异性基因的分层负调节。我们发现 TCR/CD3 和 lck 基因的表达在 T 细胞 x 成纤维细胞杂交体中消失。在杂种中，编码淋巴特异性转录因子的基因，如LEF-1、TCF-1、GATA-3、Ikaros/LyF-1、Elf-1、Myb和Fli-1的表达被消除，而编码普遍表达的转录因子的基因，如Sp-1、Ets-2和CREB的表达没有被消除。这些结果表明，假定 成纤维细胞中的阻遏蛋白会关闭几种淋巴特异性转录因子的表达，从而下调 T 细胞特异性结构基因的表达。

项目成果

Tsuneyuki OIKAWA et al.: "Suppression of lck protooncogene expression in murine somatic cell hybrids between T lymphoma cells and fibroblasts" Cytogenetics Cell Genetics. 72. 12-19 (1996)

Tsuneyuki OIKAWA 等人：“T 淋巴瘤细胞和成纤维细胞之间的小鼠体细胞杂种中 lck 原癌基因表达的抑制”细胞遗传学细胞遗传学。

Y.Kubota, K.Murakami-Murofushi, Y.Shimada, T.Ogiu and T.Oikawa: "Reduced fidility of DNA synthesis in cell extracts from chemically induced primary thymic lymphomas of mice" Cancer Reserch. 55. 3777-3780 (1995)

Y.Kubota、K.Murakami-Murofushi、Y.Shimada、T.Ogiu 和 T.Oikawa：“化学诱导的小鼠原发性胸腺淋巴瘤细胞提取物中 DNA 合成的保真度降低”癌症研究。

Yoshiko KUBOTA et al.: "Reduced fidility of DNA synthesis in cell extracts form chemically induced thymic lymphomas of mice" Cancer Research. 55. 3777-3780 (1995)

Yoshiko KUBOTA 等人：“化学诱导小鼠胸腺淋巴瘤细胞提取物中 DNA 合成保真度降低”癌症研究。

T.Oikawa, T.Yamada, Y.Kubota, Y.Hitomi, F.Uchiumi and T.Yamamoto: "Suppression of lck protooncogene expression in murine somatic cell hybrids between T lymphoma cells and fibroblasts" Cytogenet.Cell Genet.72. 12-19 (1996)

T.Oikawa、T.Yamada、Y.Kubota、Y.Hitomi、F.Uchiumi 和 T.Yamamoto：“T 淋巴瘤细胞和成纤维细胞之间的小鼠体细胞杂交中 lck 原癌基因表达的抑制”Cytogenet.Cell Genet.72。

Tsuneyuki OIKAWA: "Suppression of lck protooncogene expression in murine somatic cell hybrids between T lymphoma cells and fibroblasts" Cytogenetics Cell Genetics. 72. 12-19 (1996)

Tsuneyuki OIKAWA：“T 淋巴瘤细胞和成纤维细胞之间的小鼠体细胞杂种中 lck 原癌基因表达的抑制”细胞遗传学细胞遗传学。