Tissue-specific expression of the rearranged c-myc in murine plasmacytomas

重排c-myc在小鼠浆细胞瘤中的组织特异性表达

• 批准号: 62570152
• 负责人: OIKAWA Tsuneyuki
• 金额: $ 1.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570152/
• 关键词: Mouse plasmacytoma; C-myc; Cell fusion; Hybrid; Tissue-specificity; DNase I感受生; 組織特異性; DNase I感受性; 組織特異的因子; DNaseI感受性

In a mouse plasmacytoma, not only the rearranged c-myc but also the non-rearranged c-myc was deregulated to be unresponsive to serum stimulation. Expression of the rearranged c-myc in the plasmacytoma was repressed after cell fusion with normal fibroblasts accompanied with suppression of tumorigenic activity. This repression of the rearranged c-myc could be also found in hybrids between plasmacytoma and liver cells or T-cell lymphoma but not in hybrids between plasmacytoma and B-cells, indicating involvement of tissue specific factors in the repression. Run-on assay showed the repression was at a transcriptional level. The repression was parallel to loss of plasmacytoma-specific proteins and reduced DNase I sensitivity of the rearranged c-myc in the hybrids. These results suggest that trans-acting factors from non-B cells act directly or indirectly to the rearranged c-myc in plasmacytomas to change chromatin configulation around the gene from open state to closed one.

在小鼠浆细胞瘤中，不仅重排的c-myc，而且未重排的c-myc也被解除调控，对血清刺激无反应。与正常成纤维细胞融合后，原浆细胞瘤中重排的c-myc的表达受到抑制，并伴有致瘤活性的抑制。这种对重排c-myc的抑制也可以在浆细胞瘤与肝细胞或t细胞淋巴瘤的杂交中发现，但在浆细胞瘤与b细胞的杂交中没有发现，这表明组织特异性因素参与了这种抑制。连续试验表明，抑制是在转录水平。这种抑制与浆细胞瘤特异性蛋白的缺失是平行的，并且降低了杂交中重排的c-myc的dna酶I敏感性。这些结果表明，来自非b细胞的反式作用因子直接或间接作用于浆细胞瘤中重排的c-myc，将基因周围的染色质构型从开放状态改变为关闭状态。

项目成果

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