Investigation of Vaccination Effect by Hsp 70-Malaria Antigen Peptide Complex

热休克蛋白70-疟疾抗原肽复合物的疫苗接种效果研究

• 批准号: 06670259
• 负责人: UDONO Heiichiro
• 金额: $ 1.28万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670259/
• 关键词: Mice Malaria; Plasmodium berghei; CS protein; Killer T cell; Heat Shock Protein 70; マラリア; P.bergfei; 熱ショック蛋白; CTLエピトープ; ワクチン

For establishment of preerythrocytic vaccination model against mice malaria Plasmodium berghei, we tried to induce cellular immunity to CS protein of plasmodium berghei.1.Cytotoxic T cell epitope, NDDSYIPSAEKI which is recognized in a context with H-2 K^d by CTL lines against CS protein of Plasmodium berghei was biochemically synthesized. This 12mer peptide and heat shock protein 70 were mixed and incubated for 1 hour ar 37ﾟC for making the hsp70-peptide.2.BALB/c mice were immunized with 10mg (per mouse) of this hsp70-peptide complex twice at a week interval and spleens were removed and suspended cells were incubated with the peptide for five days in 5% CO_2,37ﾟC.Cytotoxic activity of the effector cells was assayd with ^<51>Cr labelled P815 target cells pulsed with the peptide.3.By in vitro stimulation with the different dose, 1 x 10^<-4>,1 x 10^<-5>,1 x 10^<-6>,1 x 10^<-7>,1 x 10^<-8> mol of the peptide, optimal concentrations of the peptide were identified as 1 x 10^<-6> and 1 x 10^<-7> mol for induction of primary CTLs.4.Incubation of spleen cells with the peptide in the presence of IL2 revealed no mounting effect of cytotoxic activity was observed, rather IL2 decreased the activity.5.Since repeated stimulation of effector cells with the peptide apparently increased the cytolytic activity, establishment of long term cultured cell lines were on going now.

For establishment of pre-throcytic vacuumination model against mice malaria Plasmodium berghei, we tried to induce cellular immunology to CS protein of plasmodium berghei.1.Cytotoxic T cell epitope, NDDSYIPSAEKI which is recognized in a context with H-2 K^d by CTL lines against CS protein of Plasmodium berghei was biochemically合成。将这种12mer肽和热激蛋白70混合并孵育1小时AR 37°C，以使Hsp70肽进行HSP/C小鼠，用该HSP70肽复合物的10mg（每只小鼠）以每周两次的间隔和脾脏的间隔和悬浮液的速度进行5％CO的5％CO驱动C. effector cells was assayed with ^<51>Cr labelled P815 target cells pulsed with the peptide.3.By in vitro stimulation with the different dose, 1 x 10^<-4>,1 x 10^<-5>,1 x 10^<-6>,1 x 10^<-7>,1 x 10^<-8> mol of the peptide, optimal concentrations of the peptide were identified as 1 x 10^<-6> and 1 x 10^<-7>摩尔用于诱导初级CTL。4。在IL2存在下卵形细胞与肽的结合，发现没有观察到细胞毒性活性的安装效应，而IL2降低了活性。5。降低了肽的反复刺激效果细胞与肽显然增加了长期的细胞活性细胞的建立，现在已经逐渐培养了。

项目成果

鵜殿 平一郎: "腫瘍細胞熱ショック蛋白の抗腫瘍効果における役割-腫瘍拒絶抗原としての熱ショック蛋白-" 別冊 医学の歩み 免疫疾患-state of arts-. 239-242 (1995)

Heiichiro Udono：“肿瘤细胞热休克蛋白在抗肿瘤作用中的作用 - 热休克蛋白作为肿瘤排斥抗原”单独卷，State of Arts，239-242（1995）

Srivastava P.K.,Udono H.: "Heat shock protein-peptide complexes in cancer immunotherapy." Curr.Opin.Immunol.6. 728-732 (1994)

Srivastava P.K.、Udono H.：“癌症免疫治疗中的热休克蛋白-肽复合物。”

Udono,H.,Srivastava,P.K.: "Comparison of Tumor-specific immunogenicities of stress-induced proteins gp96,hsp90,and hap 70." J.Immunol.152. 5398-5403 (1994)

Udono,H.,Srivastava,P.K.：“应激诱导蛋白 gp96、hsp90 和 hap 70 的肿瘤特异性免疫原性的比较。”

Udono, H.: "Heat shock protein and cancer immunology" Blood Immunity Cancer. 1. 36-40 (1996)

Udono, H.：“热休克蛋白和癌症免疫学”血液免疫癌症。

Udono H.,et al.: "Heat shock proteins in cancer immunotherapy." Medical Intelligence Unit.in press.(1996)

Udono H.等人：“癌症免疫疗法中的热休克蛋白”。