Pheromone Independent Efficient Transfer System in Conjugative Plasmid of Enterococcus that the Plasmid pKG (65.1kb) was isolated from Enterococcus faecium Clinical Strain
肠球菌接合质粒中信息素非依赖性高效转移系统,从屎肠球菌临床菌株中分离出质粒pKG(65.1kb)
基本信息
- 批准号:06670283
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
KT359 was Enterococcus faecium clinical isolate, resistant to kanamycin, gentamicin, erythromycin and tetracycline, and harbors several plasmids. Plasmid pKG (65.1kb) was isolated from KT359 and encoded kanamycin and gentamicin resistance. EcoRI restriction profile of pKG exhibited five fragments A through E,and the molecular size was 50.2,11.5,2.0,0.7, and 0.7kb, respectively. The order of the fragments was clockwise order of ACDEB.The pKG transferred at high frequency in Enterococcus in broth mating. Recipient strains used in conjugative transfer of the plasmid were E.faecalis FA2-2 and JH2SS,or E.faecium KT06RF (rifampin and fuscidic acid resistance) and KT06SS (streptomycin and spectinomycin resistance) strains derived from plasmid free and drug sensitive E.faecium clinical strain of KT06. pKG transferred in E.faecalis, E.faecium strains, or between E.faecium and E.faecalis strains at a frequency of about 10^<-4>,10^<-5>, or 10^<-4> per donor cell after two hours mating, respectively. Plasmid transfers in mating experiments were not induced by exposure of the donor cell to culture filtrates of plasmid free FA2-2 or KT06RF strain. Mating aggregates were not observed visually during broth mating. Small mating aggregates of several cells were observed in the broth mating under microscopic observation, while aggregates of donor cells after being exposed to a culture filtrate of recipient strain, FA2-2 or KT06RF,were not observed even under microscope. These results indicate that there is another efficent transfer system in conjugative plasmids of Enterococcus, which is different from pheromone induced transfer system of E.faecalis plasmids.
KT 359为屎肠球菌临床分离株,对卡那霉素、庆大霉素、红霉素和四环素耐药,携带多种质粒。质粒pKG(65.1kb)是从KT 359中分离得到的,编码卡那霉素和庆大霉素抗性。pKG的EcoR Ⅰ酶切图谱显示A ~ E五个片段,大小分别为50.2,11.5,2.0,0.7和0.7kb。pKG基因片段顺序为ACDEB顺时针方向。在肉汤交配中,pKG基因在肠球菌中的转移频率较高。用于质粒接合转移的E.faecalis FA 2 -2和JH 2SS,或来自无质粒和药物敏感的屎肠球菌临床菌株KT 06的屎肠球菌KT 06 RF(利福平和梭孢酸抗性)和KT 06 SS(链霉素和壮观霉素抗性)菌株。pKG在粪肠球菌、屎肠球菌菌株中或在屎肠球菌和粪肠球菌菌株之间转移,在两小时交配后,分别以每个供体细胞约10 μ g<-4>、10 μ g<-5>或10 μ g<-4>的频率转移。将供体细胞暴露于无质粒的FA 2 -2或KT 06 RF菌株的培养物中,不会诱导交配实验中的质粒转移。在肉汤交配期间,目视未观察到交配聚集体。在显微镜观察下,在肉汤交配中观察到几个细胞的小交配聚集体,而在暴露于受体菌株FA 2 -2或KT 06 RF的培养滤液后,即使在显微镜下也没有观察到供体细胞的聚集体。这些结果表明,肠球菌接合质粒中存在着不同于粪肠球菌信息素诱导质粒转移系统的另一种有效转移系统。
项目成果
期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shuhei Fujimoto: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response" J. Bacteriol.177. 5574-5581 (1995)
Shuhei Fujimoto:“粪肠球菌接合细菌素质粒 pPD1 的物理图谱以及与信息素反应相关的决定因素的鉴定”J. Bacteriol.177。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Haruyoshi Tomitaa: "Genetic analysis of the bacteriocin determinant encoded on a conjugative plasmid pY117 (57.5kb) of Enterococcus faecalis YI17" The Kitakanto Med.J.46. 89-102 (1996)
Haruyoshi Tomitaa:“粪肠球菌 YI17 接合质粒 pY117 (57.5kb) 上编码的细菌素决定簇的遗传分析”Kitakanto Med.J.46。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
富田治芳: "腸球菌(Enterococcus faecalis)のバクテリオシンプラスミドpYI17 (57. 5kb)のバクテリオシン遺伝子のクローニングと遺伝学的解析" 北関東医学. 46. 89-102 (1996)
Haruyoshi Tomita:“粪肠球菌细菌素质粒 pYI17 (57. 5kb) 的细菌素基因的克隆和遗传分析”北关东医学 46. 89-102 (1996)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Shuhei Fujimoto: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response" J.Bacteriol.177. 5574-5581 (1995)
Shuhei Fujimoto:“粪肠球菌接合细菌素质粒 pPD1 的物理图谱以及与信息素反应相关的决定因素的鉴定”J.Bacteriol.177。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
若松恵利: "Enterococcus faecalisのバクテリオシンプラスミドpPD1のバクテリオシン産生及び接合伝達関連遺伝子の遺伝学的解析" 北関東医学. 45. 139-154 (1995)
Eri Wakamatsu:“粪肠球菌细菌素质粒 pPD1 中细菌素产生和接合转移相关基因的遗传分析”Kitakanto Igaku 45. 139-154 (1995)。
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- 影响因子:0
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IKE Yasuyoshi其他文献
IKE Yasuyoshi的其他文献
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{{ truncateString('IKE Yasuyoshi', 18)}}的其他基金
Genetic and molecular analysis of bacteriocins of enterococcus clinical isolates-a bacterial colonization factor-
肠球菌临床分离株细菌素的遗传和分子分析-细菌定植因子-
- 批准号:
19390123 - 财政年份:2007
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genetic analysis of the vancomycin resistance Enterococcus highly conjugative pMG1-1 ike plasmids.
万古霉素抗性肠球菌高度接合pMG1-1 ike质粒的遗传分析。
- 批准号:
16017216 - 财政年份:2004
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Analysis of virulence factors of enterococcus, that are adhesion, bacteriocin and vancomycin resistance.
肠球菌毒力因子粘附性、细菌素及万古霉素耐药性分析。
- 批准号:
14370089 - 财政年份:2002
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research of vancomycin resistant entercocci (VRE) of chickens and chicken farms, or hospitals in Thailand
泰国鸡和养鸡场或医院的万古霉素耐药肠球菌 (VRE) 研究
- 批准号:
12576010 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research of vancomycin resistance, adherence factors, and conjugative plasmids of enterococci
肠球菌万古霉素耐药性、粘附因子和接合质粒的研究
- 批准号:
11470068 - 财政年份:1999
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on <beta> -hemolysin toxin of Streptococcus faecalis
粪链球菌β-溶血素毒素的研究
- 批准号:
60570185 - 财政年份:1985
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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