Analysis of virulence factors of enterococcus, that are adhesion, bacteriocin and vancomycin resistance.

(1)耐万古霉素屎肠球菌临床分离株质粒细菌素32决定因子的遗传分析。用显微镜观察了粪肠球菌临床分离株对涂覆微滴板壁的固定化细胞外基质（ECMs）的粘附能力。ecm由纤维连接蛋白、层粘连蛋白、胶原I、II、IV、V、纤维蛋白原和乳铁蛋白组成。组织特异性黏附菌株AS11、AS12、AS14、AS15、HT11和HT12对人膀胱癌T24细胞和人膀胱上皮细胞表现出高效的黏附，对纤维连接蛋白、层粘连蛋白、胶原I、II、IV和V EC Ms均表现出较强的黏附，黏附水平大于10^4个细胞/ mm^2。(2)组织特异性黏附粪肠球菌菌株对人膀胱癌T24细胞具有高效黏附，同时也能黏附细胞外基质蛋白。在636株耐万古霉素屎肠球菌（VRE）中，有277株（44%）具有致菌性，193株（30.3%）对粪肠球菌和hirae具有细菌素活性。产菌菌株VRE200携带高效结合庆大霉素耐药质粒pG200 (60kb)，质粒p200B编码58个氨基酸，是细菌素的决定因子。(3)广泛传播的偶联庆大霉素耐药质粒（pmg1样）与屎肠球菌出现万古霉素耐药可能存在关联。本研究使用来自密西根大学安娜堡医学院医院的640株耐万古霉素屎肠球菌（VRE）。经PCR分析，611份为VanA， 29份为VanB。492株庆大霉素耐药菌株中，261株（53%）的庆大霉素耐药性在肉汤交配中以每个供体细胞10^<-5> ~ 10^<-6>的频率转移到粪肠杆菌中。鉴定了共轭庆大霉素耐药质粒，并根据其EcoRI酶切谱将其分为A ~ B型。每种质粒的EcoRI或NdeI限制性内切片段与从粪肠杆菌临床分离物中分离的共轭庆大霉素抗性质粒pMG1 （65.1kb）杂交，并在肉液交配中有效转移。少

(1)Genetic Analysis of the Bacteriocin 32 Determinant encoded on the Plasmid Isolated from Vancomycin Resistant Enterococcus faecium Clinical Isolate.The ability of E.faecalis clinical isolates to adhere to immobilized extracellular matrixes (ECMs) coating the walls of microtiter plates was examined by microscope. The ECMs consisted of fibronectin, laminin, collagen I, II, IV, V, fibrinogen and lactoferrin. The tissue specific adhesive strains AS11, AS12, AS14, AS15, HT11 and HT12, which show highly efficient adherence to human bladder carcinoma T24 cells and human bladder epithelial cells, showed strong adherence to each of the fibronectin, laminin, collagen I, II, IV, and V EC Ms, at levels greater than 10^4 cells per mm^2 of well surface coated by ECM.(2)Tissue Specific Adherent Enterococcus faecium Strains that show Highly Efficient Adhesion to Human Bladder Carcinoma T24 Cells also adhere to Extracellular matrix Proteins.Of the 636 vancomycin-resistant Enterococcus faecium (VRE) i … More solates, two hundred seventy-seven (44%) of the strains were bacteriocinogenic, and one hundred ninety-three (30.3%) of the strains showed bacteriocin activity against E.faecium and E.hirae. The bacteriocinogenic VRE200 strain harbored highly efficiently conjugative gentamicin resistance plasmid pG200 (60kb), and other plasmid p200B encoded 58 amino acids, which was the bacteriocin determinant.(3)Possible connection between a widely disseminated conjugative gentamicin resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in Enterococcus faecium.A total of 640 vancomycin-resistant Enterococcus faecium (VRE) isolates from the Medical School Hospital of the University of Michigan, Ann Arbor, were used in this study. Of the 611 and 29 were VanA and VanB VRE, respectively, based on PCR analysis. The gentamicin resistance of each of 261 (53%) of the 492 gentamicin-resistant strains was transferred to E.faecium at a frequency of about 10^<-5> to 10^<-6> per donor cell in broth mating. The conjugative gentamicin resistance plasmids were identified and were classified into five types (A through B) with respect to their EcoRI restriction profiles. The EcoRI or NdeI restriction fragments of each type of plasmids hybridized to the conjugative gentamicin resistance plasmid pMG1 (65.1kb), which was originally isolated from an E.faecium clinical isolate, and transfer efficiently in broth mating. Less