权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Experimental evaluation of Japanese encephalitis vaccine candidates for human use generated by recombinant technology

重组技术产生的人用乙型脑炎候选疫苗的实验评价

基本信息

批准号：
06670328
负责人：
KONISHI Eiji
金额：
$ 1.09万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670328/
关键词：
Japanese encephalitis Vaccine Antibody T lymphocytes Mouse Immunity recombinant virus

项目摘要

Recombinant Japanese encephalitis (JE) vaccine candidates based on a highly attenuated vaccinia virus (NYVAC-JEV) and a canarypox virus (ALVAC-JEV) were evaluated for their ability to induce specific antibodies and cytotoxic T lymphocytes (CTLs) in mice. Six- to eight-week-old male Balb/c mice that received one or two intraperitoneal inoculations with these JE vaccine candidates at a dose of 1 * 10^7 PFU per mouse, produced neutralizing antibody and antibodies to the envelope (E) and non-structural 1 (NS1) proteins as determined by radioimmunoprecipitation. Immunization with either of these vaccine candidates also induced JE virus-specific T lymphocytes that proliferated in response to stimulation with infectious virus and/or non-infectious viral antigens. Mice maintained detedtable levels of neutralizing antibody and JE virus-specific memory T cells for at least 6 months after immunization with NYVAC-JEV and for 4 months after immunization with ALVAC-JEV.Cells induced to proliferate after stimulation with live virus contained specific CD8^+ CTLs that lysed primary Balb/c mouse kidney cells infected with JE virus and P815 mastocytoma cells infected with a recombinant vaccinia virus expressing the premembrane (prM), E,and NS1 proteins. These CTLs also lysed P815 cells infected with vaccinia recombinants expressing prM and E,and those expressing E and NS1, but did not lyse P815 cells infected with a recombinant virus expressing only NS1, indicating that the CTLs mainly recognized E,but did not recognize NS1. These results demonstrate that both recombinant JE vaccines, NYVAC-JEV and ALVAC-JEV,induce JE virus-specific antibody and CTLs in mice.

评价了基于高度减毒的牛痘病毒（NYVAC-JEV）和金丝雀痘病毒（ALVAC-JEV）的重组日本脑炎（JE）疫苗候选物在小鼠中诱导特异性抗体和细胞毒性T淋巴细胞（CTL）的能力。以1 * 10^7 PFU/只小鼠的剂量，对6 - 8周龄雄性Balb/c小鼠进行1 - 2次腹腔接种这些乙脑候选疫苗，通过放射免疫沉淀法测定，产生中和抗体以及针对包膜（E）和非结构1（NS 1）蛋白的抗体。用这些候选疫苗中的任一种进行免疫接种也诱导JE病毒特异性T淋巴细胞，其响应于感染性病毒和/或非感染性病毒抗原的刺激而增殖。在用NYVAC-JEV免疫后至少6个月和用ALVAC-JEV免疫后4个月，小鼠的中和抗体和JE病毒特异性记忆T细胞保持可检测水平。c用JE病毒感染的小鼠肾细胞和用表达前膜（prM）、E和NS 1蛋白的重组牛痘病毒感染的P815肥大细胞瘤细胞。这些CTL还裂解用表达prM和E的牛痘重组体感染的P815细胞，以及表达E和NS 1的那些细胞，但不裂解用仅表达NS 1的重组病毒感染的P815细胞，表明CTL主要识别E，但不识别NS 1。这些结果表明，两种重组乙脑疫苗，NYVAC-JEV和ALVAC-JEV，在小鼠中诱导乙脑病毒特异性抗体和CTL。