Spontaneous inhibition of HTL V-I expression in the peripheral blood

外周血中 HTL V-I 表达的自发抑制

• 批准号: 06670333
• 负责人: KANNAGI Mari
• 金额: $ 0.58万
• 依托单位: Tokyo Medical and Dental University (1995)Kumamoto University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670333/
• 关键词: HTLV-I; Antigen expression; ATL; Plasma factor.; ウイルス抗原発現

Human T-cell leukemia virus type-I (HTLV-I) is associated with adult T cell leukemia (ATL) and HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-I tax is one of the main target antigens recognized by HTLV-I-specific cytotoxic T lymphocytes (CTL). This is advantageous for the host, because cytolysis probably occurs at an early stage of leukemogenesis.Immediate detection of CTL activity in the PBMC is unusual, because virusspecific CTL activity resulting from prior infection can be detected generally only after in vitro restimulation. A similar phenomenon has been noted in humanimmunodeficiency viruus infection, which is believed to reflect active immune stimulation in vivo. The level of HTLV-I expression is elevated in the peripheral blood of HAM/TSP patients. However, the level of HTLV-I gene expression could be spontaneously elevated in vitro. Fresh leukemia cells of ATL patients only marginally expressed HTLV-I mRNA,but showed a significant induction of HTLV-I mRNA and antigens following in vitro incubation in vitro. Such induction was observed in the PBMC of asymptomatic HTLV-I carriers and HAM/TSP patients. This suppression of HTLV-I expression, presumably mediated by a plasma factor and commonly observed in HTLV-I-infected individuals, could be one reason for the persistence of HTLV-I-infection.

人类T细胞白血病病毒I型（HTLV-I）与成人T细胞白血病（ATL）和HTLV-I相关的脊髓病/热带痉挛性下肢轻瘫（HAM/TSP）相关。HTLV-I Tax是HTLV-I特异性细胞毒性T淋巴细胞（CTL）识别的主要靶抗原之一。这对宿主是有利的，因为细胞溶解可能发生在白血病发生的早期阶段，在PBMC中立即检测到CTL活性是不常见的，因为由先前感染引起的病毒特异性CTL活性通常只能在体外再刺激后才能检测到。在人类免疫缺陷病毒感染中也观察到类似的现象，这被认为反映了体内的主动免疫刺激。HAM/TSP患者外周血中HTLV-Ⅰ表达水平升高。然而，HTLV-I基因表达水平在体外可自发升高。ATL患者的新鲜白血病细胞仅少量表达HTLV-I mRNA，但在体外孵育后显示HTLV-I mRNA和抗原的显著诱导。在无症状HTLV-I携带者和HAM/TSP患者的PBMC中观察到这种诱导。这种抑制HTLV-I的表达，推测是由血浆因子介导的，通常在HTLV-I感染的个体中观察到，可能是HTLV-I感染持续存在的原因之一。

项目成果

Kannagi,M.: "Expression of the target antigen for cytotoxic T lymphocytes on adult T cell leukemia cells." Int.J.Cancer. 54. 582-588 (1993)

Kannagi,M.：“细胞毒性 T 淋巴细胞的靶抗原在成人 T 细胞白血病细胞上的表达。”

Kannagi, M.: "Expression of the target antigen for cytotoxic T lymphocytes on adult T cell leukemia cells." Int. J. Cancer. 54. 582-588 (1993)

Kannagi, M.：“细胞毒性 T 淋巴细胞的靶抗原在成人 T 细胞白血病细胞上的表达。”

EL-Farrash,M.A.: "Isolation of human T-cell leukemia type I from a transformed T-cell line derived spontaneously from lymphocytes of a seronegative Fgyptian patient with mycosis fungoides." Blood. 86. 1842-1849 (1995)

EL-Farrash，M.A.：“从患有蕈样肉芽肿的血清阴性埃及患者的淋巴细胞自发衍生的转化 T 细胞系中分离人类 I 型 T 细胞白血病。”

神奈木 真理: "HTLV-I反応性T細胞の認識ペプチド" 臨床免疫. 26. 412-417 (1994)

Mari Kanagi：“HTLV-I 反应性 T 细胞的识别肽”《临床免疫学》26. 412-417 (1994)。

EL-Farrash,M.A.: "Isolation of human T-cell leukemia type I from a transformed T-cell line derived spontaneously from lymphocytes of a seronegative Wgyptian patient with mycosis fungoides." Blood. 86. 1842-1849 (1995)

EL-Farrash，M.A.：“从患有蕈样肉芽肿的血清阴性 Wgyptian 患者的淋巴细胞自发衍生的转化 T 细胞系中分离出人类 I 型 T 细胞白血病。”