Characterization of the HTLV-I receptor

HTLV-I 受体的表征

• 批准号: 04670276
• 负责人: KANNAGI Mari
• 金额: $ 0.32万
• 依托单位: Kumamoto University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670276/
• 关键词: HTLV-I; Virus receptor; Syncytium; Viral adsorption; ウイルス感染; HIV-I

Human T-cell leukemia virus type-I (HTLV-I) and human immunodeficiency virus type-1 (HIV-1) have a similar tropism for target cell types, especially for CD4+ T cells. Although CD4 is an HIV-1 receptor, the cellular receptor for HTLV-I is not yet known. HTLV-I does not effectively infect cells as a cell free virus, but require a cell-to-cell contact between HTLV-I infected cells and target cells. Initially, we used a syncytium formation system to detect HTLV-I infection. This system provided us an evidence that the receptors for HTLV-I and HIV-1 are totally independently present on the cell surface of human T cells. Furthermore, HTLV-I receptor seemed not to be involved in the CD4 molecule. We tried to make monoclonal antibodies for the HTLV-I receptor by using this system, but an antibody blocking HTLV-I-induced syncytium formation was not obtained yet. Besides such cell-to-cell system, we established an experimental system to detect HTLV-I adsorption, since the cell-to-cell would be more affected by adhesion molecules on the cell surface unrelated to virus receptors. HTLV-I particles were concentrated and incubated with the target cells' and viral adsorption to the cell surface was quatnitated by FACS analysis. HTLV-I could bind a wide range of human cell lines with a few exceptions. Human anti-HTLV-I sera inhibited the HTLV-I adsorption, suggesting that these sera contain antibodies neutralizing HTLV-I binding. Efforts to obtain monoclonal antibodies to the HTLV-I receptor are currently being made using these systems.

人类T细胞白血病病毒I型（HTLV-I）和人类免疫缺陷病毒I型（HIV-1）对靶细胞类型具有相似的嗜性，特别是对CD 4 + T细胞。虽然CD 4是HIV-1受体，但HTLV-I的细胞受体尚不清楚。HTLV-I作为无细胞病毒不能有效地感染细胞，但需要HTLV-I感染的细胞和靶细胞之间的细胞-细胞接触。最初，我们使用合胞体形成系统来检测HTLV-I感染。该系统为我们提供了HTLV-I和HIV-1的受体完全独立地存在于人T细胞的细胞表面的证据。此外，HTLV-1受体似乎不参与CD 4分子。我们试图通过使用该系统制备HTLV-I受体的单克隆抗体，但尚未获得阻断HTLV-I诱导的合胞体形成的抗体。除了这种细胞-细胞系统，我们建立了一个实验系统来检测HTLV-I吸附，因为细胞-细胞将更多地受到细胞表面上与病毒受体无关的粘附分子的影响。将HTLV-I颗粒浓缩并与靶细胞一起孵育，并通过FACS分析来量化病毒吸附到细胞表面。HTLV-I可以结合多种人类细胞系，只有少数例外。人抗HTLV-I血清抑制HTLV-I吸附，表明这些血清含有中和HTLV-I结合的抗体。目前正在使用这些系统努力获得针对HTLV-I受体的单克隆抗体。

项目成果

M.Kannagi,et al.: "Expression of the target antigen for cytotoxic T lymphocytes on adult T cell leukemia cells." Int J.Cancer. 54. 582-588 (1993)

M.Kannagi 等人：“细胞毒性 T 淋巴细胞的靶抗原在成人 T 细胞白血病细胞上的表达。”

H.Akari: "Sinple assay system fordetecting human T cell leukemia virus type I-bindin cells and its application in titrating binding inhibitory antibodies." Laboratory Investigation. 69. 629-634 (1993)

H.Akari：“用于检测人 T 细胞白血病病毒 I 型结合细胞的简单测定系统及其在滴定结合抑制抗体中的应用。”

M.Kannagi: "Expression of the target antigen for cytotoxic T lymphocytes on adult T lymphocytes on adult T cell leukemia cells." Int.J.Cancer. 54. 582-588 (1993)

M.Kannagi：“细胞毒性 T 淋巴细胞的靶抗原在成人 T 细胞白血病细胞上的成人 T 淋巴细胞上的表达。”

M.J.Kuroda,et al.: "Inefficient transmission of HTLV-I to MOLT-4 cells by cell-free virus and co-cultivation." Intervirology. 34. 202-212 (1993)

M.J.Kuroda 等人：“通过无细胞病毒和共培养，HTLV-I 向 MOLT-4 细胞的低效传播。”

H.Akari,et al.: "Simple assay system for detecting human T cell leukemia virus type-I-binding cells and its application in titrating binding inhibitory antibodies." Laboratory Investigation. 69. 629-634 (1993)

H.Akari 等人：“用于检测人 T 细胞白血病病毒 I 型结合细胞的简单测定系统及其在滴定结合抑制抗体中的应用。”