STUDIES ON PREDICTION AND CLASSIFICATION OF OCCUPATIONAL RESPIRATORY SENSITIZERS

职业呼吸致敏物的预测和分类研究

• 批准号: 06670387
• 负责人: AOYAMA Kohji
• 金额: $ 1.34万
• 依托单位: KAGOSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670387/
• 关键词: Occupational; Sensitizers; Respiratory; Allergy; Classification; Prediction; Cytokine; TDI; 許容濃度; 予知法

The purpose of this study was to provide the information to establish preventive measurements for respiratory allergy from occupational sensitizes. We carried out this study recognizing the requirements of preventive measurements as below : the prediction of allergenicity, listing up of occupational sensitizers and confirmation of dose-response relationship in hypersensitivity reactions. We revised our drafted table, made previously by us, to exhibit occupational respiratory sensitizers. The main revised point was that the criteria of classification was on the basis of reliability of information. To assess the criteria, we investigated whether several respiratory sensitizers selected were given the adequate assessments. Subsequently, the property of the criteria was confirmed generally. The criteria of epidemiological evidences required further revises.To develop methods to evaluate respiratory sensitization of occupational chemicals using mouse, we assayd cytokine profiles in mice exp … More osed to sensitizers. Mice were sensitized by painting of toluene diisocyanate (TDI) , known as a potent contact and respiratory allergen. PHA stimulated IL-2, IL-4, IL-5 and IFN-gamma were detected in spleens by ELISA.After one and two week following the initiation of exposure to TDI,the significant increment of total IgE and ear swelling with depending on exposure dose was found. The levels of IL-2, IL-4 and IFN-gamma in 1 and 5% TDI exposure group increased after one week compared with control group. Only IL-4 level decreased after two weeks. For the relation between sensitization reactions and cytokines, IL-4 level, related to immediate type hypersensitivity, was correlated with total IgE production. IFN-gamma, related to contact hypersensitivity, was correlated with ear swelling. Therefore, the assay of cytokine profiles was suggested to be available for the evaluation of sensitizers causing immediate and delayd type hypersensitivity. Accordingly serial methods to evaluate respiratory sensitization were settled by addition of listing up of occupational sensitizers and guinea-pig experimental model for setting up of OELs which we have developed. Less

本研究的目的是为建立职业性致敏性呼吸道过敏的预防措施提供信息。我们开展了这项研究，认识到预防措施的要求如下：预测过敏原，列出职业致敏物，确认超敏反应的剂量反应关系。我们修改了之前由我们起草的表格，以展示职业性呼吸致敏剂。修订后的主要观点是，分类标准是以信息的可靠性为基础的。为了评估标准，我们调查了所选的几种呼吸致敏剂是否得到了充分的评估。随后，这些准则的性质得到了普遍确认。流行病学证据的标准需要进一步修订。为了建立评价职业性化学物质对小鼠呼吸道致敏性的方法，我们测定了接触致敏剂较多的小鼠的细胞因子谱。小鼠通过涂甲苯二异氰酸酯（TDI）致敏，TDI是一种强效的接触和呼吸过敏原。ELISA法检测PHA刺激的脾组织中IL-2、IL-4、IL-5和ifn - γ的含量。在TDI暴露后1周和2周，总IgE和耳部肿胀随暴露剂量的增加而显著增加。1、5% TDI暴露组1周后IL-2、IL-4、ifn - γ水平较对照组升高。两周后仅IL-4水平下降。对于致敏反应与细胞因子的关系，与即时型超敏反应相关的IL-4水平与总IgE产量相关。ifn - γ与接触性超敏反应有关，与耳部肿胀相关。因此，细胞因子谱分析建议可用于评估引起立即和延迟型超敏反应的致敏剂。在此基础上，通过列举职业致敏物和建立豚鼠实验模型，确定了评价呼吸道致敏性的一系列方法。少

项目成果

松下敏夫、青山公治、胥宝会: "職業性アレルギー疾患の発症機序とその予測" 産業医学レビュー. 7. 177-199 (1995)

Toshio Matsushita、Koji Aoyama、Kehokai：“职业过敏性疾病的发病机制及其预测”《职业医学评论》7. 177-199 (1995)。

Kohji Aoyama et al.: "Classification of sensitizers inducing occupational asthma." Arch Environ contam Toxicol. (1996)

Kohji Aoyama 等人：“诱发职业性哮喘的敏化剂分类。”

Kohji Aoyama et al.: "Classification of sensitizers inducing occupational asthma." Arch. Environ.Contam.Toxicol.(1996)

Kohji Aoyama 等人：“诱发职业性哮喘的敏化剂分类。”

Kohji Aoyama et al.: "Classification of sensitizers inducing occupational asthma." Arch. Environ. Contam. Toxicol.(1996)

Kohji Aoyama 等人：“诱发职业性哮喘的敏化剂分类。”

Kohji Aoyama et al.: "Mitogen stimulated cytokine production in mice sensitized with toluene diisocyanate." Arch Environ contam Toxicol. (1996)

Kohji Aoyama 等人：“丝裂原刺激甲苯二异氰酸酯致敏的小鼠中细胞因子的产生。”