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Fundamental Study on Prediction and Threshold Limit Values of Occupational Allergens

职业过敏原预测及阈值的基础研究

基本信息

批准号：
01570294
负责人：
AOYAMA Kohji
金额：
$ 1.34万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570294/
关键词：
Occupational allergen Threshold limit values Allergy Inhalation sensitizing experiment Dose-effect relationship Prediction of allergencity TDI TMA 呼吸器皮膚感作予知試験 chlorothalonil アレルギ-反応

项目摘要

First, we collected data and articles on lists of occupational exposure from foreign countries and listed occupational sensitizers by the sites of allergic reaction on the basis of them. Further, we classified the occupational sensitizers into three subgroups according to the quality and quantity of evidence that supported the allergenicity of them. We finished to prepare the proposition of additional lists, with the article list, of the list of occupational exposure limits in Japan. Next, we carried out the inhalation exposure experiments using TDI to obtain the fundamental data for setting of occupational exposure limits and for predicting of occupational sensitizers. Subsequently, the increase in basophil degranulation was observed depending on the concentrations (0.2, 0.02, 0 ppm) of TDI in the induction. The results indicated the dose-effect relationship in the production of specific antibody to TDI. The changes of respiratory rates also depended on the concentration. 0.02 ppm of TDI, which is the occupational exposure limit in Japan, failed to sensitize guinea pigs. We examined the appropriateness of our experiment methods as the prediction test and the methodology of setting of threshold limit values using TPN and TMA. Allergic reactions was induced by inhaled TPN or TMA exposure depending on the concentration of them.These series of experiment research suggested the usefulness of our experimental model for setting of threshold limit values of occupational sensitizers and for predicting of allergenicity. Further experiment research is necessary to standardize this experimental model and to examine the methods with the simple and sharp detection.

首先收集国外有关职业暴露名录的资料和文献，并在此基础上按过敏反应部位列出职业致敏剂。此外，我们根据支持其过敏性的证据的质量和数量将职业增敏剂分为三个亚组。我们完成了日本职业接触限值清单的附加清单的提议以及文章清单。接下来，我们利用TDI进行了吸入暴露实验，为制定职业接触限值和预测职业致敏剂提供了基础数据。随后，观察到嗜碱性粒细胞脱颗粒的增加取决于诱导过程中TDI的浓度(0.2，0.02，0ppm)。结果表明，TDI特异性抗体的产生存在剂量-效应关系。呼吸速率的变化也依赖于浓度。0.02ppm的TDI是日本的职业接触限值，但未能使豚鼠致敏。我们检验了我们的实验方法作为预测测试的适当性，以及使用TPN和TMA设置阈值限值的方法。吸入TPN或TMA可引起过敏反应，这取决于它们的浓度。这一系列的实验研究表明，我们的实验模型对于设定职业致敏剂的阈值限值和预测过敏性是有用的。有必要进行进一步的实验研究，以规范这一实验模型，并用简单而敏锐的检测来检验方法。