AN IMPROVED PROCEDURE FOR SAMPLING MUTAGENS BY HANGING BLUE RAYON IN AQUATIC ENVIRONMENT

水生环境中悬挂蓝色人造丝诱变剂取样的改进方法

• 批准号: 06670380
• 负责人: KIRA Shohei
• 金额: $ 1.15万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670380/
• 关键词: AQUATIC ENVIRONMENT; MUTAGENICITY; MONITORING; POLYCYCLIC AROMATICS; INTENCITY OF WATER STREAM; GYPSUM BALL; CORRECTION; BLUE RAYON; 石コウ球; 変異原性物質; 多環芳香族炭化水素; Benzo(a)pyrene; 時間平均値

For monitoring polycyclic mutagens in water, we proposed a sampling technique : hanging blue rayon (an adsorbent selective for polycyclic compund) in water sites. The amount measured by this technique may be affected by water movement during hanging, although the procedure is simple and informative. Here, we have improved this technique more quantitatively, through correcting the amount by the intensity of water stream.(1) We used benzo (a) pyrene (BaP) as a model for polycyclic mutagen. Studies in an experimental aquarium showed a hanging blue rayon is an efficient monitor for BaP in water, however the amount adsorbed increases when the water is stirred vigorously.(2) We applied a gypsum-ball method proposed by Komatsu as a simple measurement for intensity of water movement. A reduced eight during a period of 24-hr hanging (Wd) correlated well with the average flow of water measured by an electromagnetic flow meter. Therefore a simple use of measured Wd for correcting the amount of Ba … More P is equivalent to the correction by the intensity when the procedure is accompanied by hanging blue rayon in water.(3) We set up a portable sampler for BaP in water, through immersed Sep-Pak C18 as an adsorbent, the water was continuously passed by a battery-operated pump. We could measure ng/L-level of BaP in environmental waters as the time-weighted average (TWA-BaP) by the sampler, however the apparatus is expensive.(4) We measured the amount of BaP adsorbed by blue rayon (BR-BaP) and Wd of gypsum-ball during similutaneous hanging. A corrected value calculated by deviding BR-BaP with Wd showed good correlation with TWA-BaP (correlation coefficients were 0.865 with correction and 0.818 without). The correlation equation given here was {(TWA-BaP)=6.5x(BR-BaP)/(Wd)}. The results suggest that the correction improves accuracy of measurement quantitatively.(5) We selected 2 sampling sites : one was a port surrounded by big industries and the other was far from the origin of pollution. Corrected values of water-borne BaP at the former site ranged from 0.14 to 0.63 ng/gBR/gGypsum and those at the latter site was less than 0.16. The results explain the former site was more polluted by BaP than the latter in contrast. Less

为了监测水中的多环致突变物，我们提出了一种采样技术：在水点悬挂蓝色人造丝(一种对多环化合物具有选择性的吸附剂)。这项技术测量的量可能会受到悬挂过程中水运动的影响，尽管过程简单且信息丰富。在这里，我们通过水流强度校正量，对这一技术进行了更定量的改进。(1)我们使用苯并(A)芘(BaP)作为多环诱变剂的模型。在实验水族馆中的研究表明，悬挂的蓝色人造丝是水中BaP的有效监测器，但当水被大力搅拌时，吸附量增加。(2)我们应用了小松提出的石膏球方法作为水运动强度的简单测量。在24小时悬吊(WD)期间减少的8与电磁流量计测量的平均水流量有很好的相关性。因此，简单地用测得的WD改正Ba…的量多P相当于在水中悬挂蓝色人造丝时的强度校正。(3)我们建立了一种便携式水中BaP采样器，通过浸泡的Sep-Pak C18作为吸附剂，水由电池驱动的泵连续通过。我们可以用采样器测量环境水体中BaP的ng/L水平作为时间加权平均值(TWA-BaP)，但仪器价格昂贵。(4)测量了相同悬挂过程中蓝色粘胶吸附BaP的量(BR-BaP)和石膏球的WD。将BR-BaP与WD分开计算的校正值与TWA-BaP具有良好的相关性(校正后相关系数为0.865，未校正时相关系数为0.818)。给出的关联式为：{(TWA-Bap)=6.5x(BrBap)/(Wd)}。结果表明，修正从定量上提高了测量的准确性。(5)我们选择了两个采样点：一个是大型工业港，另一个是远离污染源的港口。前一处水体BaP的校正值为0.14~0.63 ng/gbr/石膏，后一处水体BaP的校正值小于0.16。结果表明，前者的BaP污染程度高于后者。较少

项目成果

堀口 洋.野上 祐作.吉良 尚平: "水環境中の変異原性検出手法の改良" 第29回日本水環境学会年会講演集. 309 (1995)

Hiroshi Horiguchi、Yusaku Nogami 和 Shohei Kira：“水环境致突变性检测方法的改进”日本水环境学会第 29 届年会记录 309 (1995)。

堀口 洋.野上 祐作.吉良 尚平: "ブルーレ-ヨン懸垂法による水環境中のサンプリング手法の改良" 第30回日本水環境学会年会講演集. 257 (1996)

Hiroshi Horiguchi、Yusaku Nogami 和 Shohei Kira：“使用 Blue Rayon 悬浮法改进水环境中的采样方法”日本水环境学会第 30 届年会记录 257 (1996)。

Shohei KIRA et al: "Comparison of techniques for monitoring waterborne mutagens : Efficiency of blue rayon, SepPak C18, and a biota, corbicula, in concentrating benzo (a)-pyrene in a model water system" Bull Environ Contam Toxicol. 57, (Aug), (in press).

Shohei KIRA 等人：“监测水性诱变剂的技术比较：蓝色人造丝、SepPak C18 和生物群、蚬类在模型水系统中浓缩苯并 (a)-芘的效率”Bull Environ Contam Toxicol。

坂野 雅彦.野上 祐作.吉良 尚平: "水環境中の多環芳香族炭化水素の時間平均値の測定手法に関する研究" 第29回日本水環境学会年会講演集. 374 (1995)

Masahiko Sakano. Shohei Kira：“水环境中多环芳烃的时间平均值测量方法的研究”日本水环境学会第29届年会记录374（1995）。

Shohei Kira et al: "A comparative study on the technique for monitoring water-borne mutagens, by the use of blue rayon, Sep-PaK,and biota, corbicula" Abstract of 23th annual meeting of Environ Mut Soc Jpn. 68. (1994)

Shohei Kira 等人：“使用蓝色人造丝、Sep-PaK 和生物群、蚬进行水性诱变剂监测技术的比较研究”Environ Mut Soc Jpn 第 23 届年会摘要。