Development of a method for measurement of a time-weighted average concentration of polycyclic mutagens in marine environment-for practiced utilization in monitoring of marine environment.

开发一种测量海洋环境中多环诱变剂时间加权平均浓度的方法 - 用于海洋环境监测的实际应用。

• 批准号: 11557030
• 负责人: KIRA Shohei
• 金额: $ 5.82万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557030/
• 关键词: mutagenicity; polycyclic aromatic compounds; water environment; time-weighted average concentration; sampling device; blue rayon; water flow intensity; 補集装置

(1) Results of measuring the time-weighted average (TWA) concentration of Benzo (a) pyrene (BaP) in the Seto Inland Sea, Japan.The values of the TWA concentration of BaP from 1999 to 2000 ranged from 0.16 to 8.77 ng/L for the sampling sites in Okayama seashore, from 0.08 to 7.04 ng/L for Osaka Bay sites and from undetectable level (0.01 ng/L) to 18.88 ng/L for the sites in Northern Kyushu.(2) Effects of water temperature on the decrease in weight of plaster balls.We gave a dissolving test by hanging a plaster ball in moving water and found that the decrease in weight of the plaster ball correlated well with water flow intensity, and the regression coefficient and the intercept of regression line were affected by water temperature. We obtained the following equation :C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)where C is water flow intensity (cm/sec), ΔW is the decrease in weight of plaster ball (g) and T is water temperature (℃) and Therefore, according to this equation, it becomes possibl … More e to correct the value of measured TWA if water temperature is known.(3) Preserving condition of blue rayon after hanging and the recovery rate of BaP.One nanogram of BaP was dissolved in distilled water in a beaker and let BaP adsorbed to BR.We then calculated the recovery rate from amount of BaP after keeping it under various conditions against the initial one that was obtained from extraction without preservation. They were 101.6±8.2% (for one week) and 100.0±6.8% (for two weeks) when BR was preserved with aluminium-foil-wrapping at -5℃, and 93.5±5.1% (for four weeks) when preserved with transparent-plastic-foil-wrapping at 15-2O℃. The amount of the BaP adsorbed to BR has maintained over 90% even in the room temperature. Our study provides a convenient and a practical procedure for long-distance transportation of the samples.(4) Relationship between the amount of BaP and mutagenicity in samples after BR hanging.We have reported that mutagenicity detected by fhe BR hanging technique positively correlated with the amount of BaP.The present study unexpectedly found some samples despite of small amount of BaP showed high mutagenicity. Most of these phenomena were observed on the samples hanging in estuaries of the rivers flowing through the cities. This fact suggests that the city sewages might contain highly mutagenic substances except for BaP.(5) Publications and presentation of the study.We have published and presented the study results in scientific journals and at some conferences (see references). Furthermore, we are preparing for presentations at following international symposiums in order to receive various evaluations from foreign scientists : (1) PRIMO11 (July 2001, Plymouth, UK), (2) ISTA10 (August 2001, Quebec, Canada), (3) 8thICEM (October 2001, Shizuoka, Japan). Less

(1)日本濑户内海苯并(a)芘（BaP）时间加权平均（TWA）浓度测定结果。1999 ~ 2000年，冈山海岸各采样点的水体中酚类物质的TWA浓度为0.16 ~ 8.77 ng/L，大阪湾采样点为0.08 ~ 7.04 ng/L，九州北部采样点的水体中酚类物质的TWA浓度为0.01 ~ 18.88 ng/L。(2)水温对石膏球减重的影响。通过将石膏球悬挂在流动水中进行溶解试验，发现石膏球重量的下降与水流强度有良好的相关性，并且回归系数和回归线截距受水温的影响。得到：C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)，其中C为水流强度（cm/sec）， ΔW为石膏球重量减少量(g)， T为水温（℃），因此，根据该式可以…(3)蓝色人造丝悬挂后的保存状况及BaP的回收率。将1纳克的BaP溶解在烧杯蒸馏水中，让BaP吸附在BR上。然后，我们计算了不同条件下保存后的BaP的回收率，并与未保存提取的初始回收率进行比较。BR在-5℃用铝箔包膜保存1周时为101.6±8.2%，在2周时为100.0±6.8%，在15- 20℃用透明塑料膜包膜保存4周时为93.5±5.1%。在室温条件下，BaP在BR上的吸附量仍保持在90%以上。我们的研究为样品的长途运输提供了一种方便实用的方法。(4) BR悬挂后样品中BaP含量与致突变性的关系。我们报道了BR悬挂技术检测的致突变性与BaP的含量呈正相关。本研究意外地发现，尽管少量的BaP样本具有高诱变性。这些现象大多是在流经城市的河流河口悬挂的样品上观察到的。这一事实表明，城市污水中可能含有除BaP外的高度诱变物质。(5)研究发表的刊物及报告。我们已经在科学期刊和一些会议上发表并展示了研究结果（见参考文献）。此外，我们正准备在以下国际研讨会上发表报告，以获得外国科学家的各种评价：(1)PRIMO11（2001年7月，英国普利茅斯），(2)ISTA10（2001年8月，加拿大魁北克），(3)8thICEM（2001年10月，日本静冈）。少

项目成果

Kira S.: "Measurement of benzo (a) pyrene in sea water and in mussels in the Seto inland sea, Japan"Bull.Environ.Contam.Toxicol. 65(5). 631-637 (2000)

Kira S.：“日本濑户内海海水和贻贝中苯并 (a) 芘的测量”Bull.Environ.Contam.Toxicol。

S.KIRA: "MEASUREMENT OF BENZO(A)PYRENE IN SEA WATER AND IN MUSSELS IN THE SETO INLAND SEA, JAPAN"9th International Symposium on Toxicity Assessument Programme and Abstracts. -25 (1999)

S.KIRA：“日本濑户内海海水和贻贝中苯并（A）芘的测量”第九届国际毒性评估计划和摘要研讨会。

T.Komatsu: "Measurement of water flow in seagrass meadows or seagrass forests with plaster balls of gypsum."2nd Open Seminer in Workshop for Scientific Diving among Japan, France, US and Australia, Japan Marine Science and Technology Center, Ishigaki, Oki

T.Komatsu：“用石膏球测量海草草甸或海草森林中的水流。”日本、法国、美国和澳大利亚之间的科学潜水研讨会第二届公开研讨会，日本海洋科学技术中心，石垣岛，大木

Furushima Y.: "Attempt to achieve simple measurement of water motion in shallow areas."OCEANS 2000 MTS/IEEE Proceedings. 2. 993-997 (2000)

Furushima Y.：“尝试实现浅层区域水运动的简单测量。”OCEANS 2000 MTS/IEEE 论文集。

早津彦哉: "ポリマー担体に固着した色素の反応:ブルーレーヨン、ブルーキチン、グリーンキトサンなどの作製とその応用"薬学雑誌. 120(6). 534-547 (2000)

Hikoya Hayatsu：“固定在聚合物载体上的染料的反应：蓝色人造丝、蓝色甲壳素、绿色壳聚糖等的制备及其应用”，Pharmaceutical Journal 120(6) 534-547 (2000)。