Development of a method for measurement of a time-weighted average concentration of polycyclic mutagens in marine environment-for practiced utilization in monitoring of marine environment.

开发一种测量海洋环境中多环诱变剂时间加权平均浓度的方法 - 用于海洋环境监测的实际应用。

• 批准号: 11557030
• 负责人: KIRA Shohei
• 金额: $ 5.82万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557030/
• 关键词: mutagenicity; polycyclic aromatic compounds; water environment; time-weighted average concentration; sampling device; blue rayon; water flow intensity; 補集装置

(1) Results of measuring the time-weighted average (TWA) concentration of Benzo (a) pyrene (BaP) in the Seto Inland Sea, Japan.The values of the TWA concentration of BaP from 1999 to 2000 ranged from 0.16 to 8.77 ng/L for the sampling sites in Okayama seashore, from 0.08 to 7.04 ng/L for Osaka Bay sites and from undetectable level (0.01 ng/L) to 18.88 ng/L for the sites in Northern Kyushu.(2) Effects of water temperature on the decrease in weight of plaster balls.We gave a dissolving test by hanging a plaster ball in moving water and found that the decrease in weight of the plaster ball correlated well with water flow intensity, and the regression coefficient and the intercept of regression line were affected by water temperature. We obtained the following equation :C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)where C is water flow intensity (cm/sec), ΔW is the decrease in weight of plaster ball (g) and T is water temperature (℃) and Therefore, according to this equation, it becomes possibl … More e to correct the value of measured TWA if water temperature is known.(3) Preserving condition of blue rayon after hanging and the recovery rate of BaP.One nanogram of BaP was dissolved in distilled water in a beaker and let BaP adsorbed to BR.We then calculated the recovery rate from amount of BaP after keeping it under various conditions against the initial one that was obtained from extraction without preservation. They were 101.6±8.2% (for one week) and 100.0±6.8% (for two weeks) when BR was preserved with aluminium-foil-wrapping at -5℃, and 93.5±5.1% (for four weeks) when preserved with transparent-plastic-foil-wrapping at 15-2O℃. The amount of the BaP adsorbed to BR has maintained over 90% even in the room temperature. Our study provides a convenient and a practical procedure for long-distance transportation of the samples.(4) Relationship between the amount of BaP and mutagenicity in samples after BR hanging.We have reported that mutagenicity detected by fhe BR hanging technique positively correlated with the amount of BaP.The present study unexpectedly found some samples despite of small amount of BaP showed high mutagenicity. Most of these phenomena were observed on the samples hanging in estuaries of the rivers flowing through the cities. This fact suggests that the city sewages might contain highly mutagenic substances except for BaP.(5) Publications and presentation of the study.We have published and presented the study results in scientific journals and at some conferences (see references). Furthermore, we are preparing for presentations at following international symposiums in order to receive various evaluations from foreign scientists : (1) PRIMO11 (July 2001, Plymouth, UK), (2) ISTA10 (August 2001, Quebec, Canada), (3) 8thICEM (October 2001, Shizuoka, Japan). Less

(1)日本濑户内海苯并（a）芘（BaP）的时间加权平均（TWA）浓度测量结果：1999 - 2000年，冈山沿岸采样点BaP的TWA浓度值为0.16 - 8.77 ng/L，大坂湾研究中心的浓度范围为0.08 - 7.04 ng/L，九州北方研究中心的浓度范围为不可检测水平（0.01 ng/L）-18.88 ng/L。(2)水温对石膏球失重的影响通过石膏球悬于流水中的溶解试验，发现石膏球失重与水流强度有很好的相关性，回归系数和回归直线的截距受水温的影响。得到以下公式：C={ΔW-（0.1232T+0.2247）}/（0.0189T+0.8162）式中C为水流强度（cm/sec），ΔW为石膏球的重量减少量（g），T为水温（℃）。 ...更多信息 e.如果水温已知，则校正测量的TWA值。(3)蓝丝悬后的保存条件及BaP的回收率将1 ng BaP溶于烧杯中的蒸馏水中，使BaP吸附于BR上，以不同条件下保存后的BaP量与未保存时提取的BaP量比较，计算回收率。在-5℃条件下，用铝箔包裹的BR保存1周和2周时，其保存率分别为101.6±8.2%和100.0±6.8%;在15- 20 ℃条件下，用塑料薄膜包裹的BR保存4周时，其保存率为93.5±5.1%。在室温下BR对BaP的吸附量仍保持在90%以上。本研究为样品的远距离运输提供了一种方便实用的方法。(4)BR挂片法测定样品中BaP含量与致突变性的关系我们曾报道用BR挂片法测定的致突变性与BaP含量呈正相关，本研究意外地发现有些样品尽管BaP含量很低，但仍显示出很高的致突变性。这些现象大多是在流经城市的河流河口悬挂的样品上观察到的。这一事实表明，城市污水中可能含有除苯并[a] P以外的高致突变物质。(5)研究的出版物和报告：我们已经在科学期刊和一些会议上发表和介绍了研究结果（见参考文献）。此外，我们正在准备在以下国际研讨会上发表报告，以接受外国科学家的各种评价：（1）PRIMO 11（2001年7月，普利茅斯，英国），（2）ISTA 10（2001年8月，魁北克，加拿大），（3）8 thICEM（2001年10月，静冈，日本）。少

项目成果

Kira S.: "Measurement of benzo (a) pyrene in sea water and in mussels in the Seto inland sea, Japan"Bull.Environ.Contam.Toxicol. 65(5). 631-637 (2000)

Kira S.：“日本濑户内海海水和贻贝中苯并 (a) 芘的测量”Bull.Environ.Contam.Toxicol。

S.KIRA: "MEASUREMENT OF BENZO(A)PYRENE IN SEA WATER AND IN MUSSELS IN THE SETO INLAND SEA, JAPAN"9th International Symposium on Toxicity Assessument Programme and Abstracts. -25 (1999)

S.KIRA：“日本濑户内海海水和贻贝中苯并（A）芘的测量”第九届国际毒性评估计划和摘要研讨会。

T.Komatsu: "Measurement of water flow in seagrass meadows or seagrass forests with plaster balls of gypsum."2nd Open Seminer in Workshop for Scientific Diving among Japan, France, US and Australia, Japan Marine Science and Technology Center, Ishigaki, Oki

T.Komatsu：“用石膏球测量海草草甸或海草森林中的水流。”日本、法国、美国和澳大利亚之间的科学潜水研讨会第二届公开研讨会，日本海洋科学技术中心，石垣岛，大木

Furushima Y.: "Attempt to achieve simple measurement of water motion in shallow areas."OCEANS 2000 MTS/IEEE Proceedings. 2. 993-997 (2000)

Furushima Y.：“尝试实现浅层区域水运动的简单测量。”OCEANS 2000 MTS/IEEE 论文集。

早津彦哉: "ポリマー担体に固着した色素の反応:ブルーレーヨン、ブルーキチン、グリーンキトサンなどの作製とその応用"薬学雑誌. 120(6). 534-547 (2000)

Hikoya Hayatsu：“固定在聚合物载体上的染料的反应：蓝色人造丝、蓝色甲壳素、绿色壳聚糖等的制备及其应用”，Pharmaceutical Journal 120(6) 534-547 (2000)。