Degeneration of Machine oils and the generation of toxicity during Their Recycled use- Studies from the view point of industrial health.

机械油在金属加工厂中广泛使用，在工作场所中，水溶性或乳化型机油已成为主要类型。在回收使用过程中，金属表面的高温和高压可能会在使用前无法预测的新有毒物质的产生中发挥作用。目前的研究涉及随着循环使用时间的推移，对切削液中诱变性的检测和监测，以及工作场所油雾的测量。在蓝棉提取后，这里测试的11种未使用的水溶性切削液中的一种在没有代谢激活的情况下，通过与TA98沙门氏菌的Ames试验检测到了诱变活性。2.经Sep-Pak C18提取后，未使用的和循环使用的水溶性切削液中的极性组分对TA100具有致突变性，且未被代谢激活。3.随时间推移收集的部分机油在使用60-90天后表现出致突变性。4.作为便携式油雾采样器，我们测试了内置两级的TR型采样器的一个头部，并采集了污渍-钢板上的油雾。我们还测试了一种油量计，用于测量用CC14萃取后收集在盘子上的油成分。上述结果表明，通过环境控制系统，对环境空气中的油类水平和液体中的毒性(如致突变性)进行监测，可以保护机械加工车间工人的健康。

Machine oils are widely used in metal processing factories and a water soluble or emulsifiable type has become predominant in the work place. During their recycled use process, a high temperature and a high pressure on the metal surface could play a role in generating new toxic substances unpredicted before use. The present studies concern the detection of mutagenicity and its monitoring in cutting fluids with the lapse of time of recycled use, and the measurement of oil mists in the work places.1. After the blue cotton extraction, one of 11 unused water soluble cutting fluids tested here showed mutagenic activity detected by the Ames test with Salmonella TA98 without metabolic activation. 2. With the Sep-Pak C18 extraction, polar fractions of an unused and a recycle-used water soluble cutting fluids showed mutagenicity by TA100 without metabolic activation. 3. Some machine oils being collected as the lapse of time showed mutagenicity after 60-90 days of use. 4. As a portable sampler for oil mists, we tested a head of the TR sampler with two stages built in, and collected the oil mists on stainles-steel plate. We also tested an oil meter for the measurement of oil components collected on the plate after extraction with CC14. The results were well corresponded to those with the low volume sampler, which collects the oil mists in a glass fiber filter and is a conventional method.The above results suggested that monitorings of both the levels of oils in the ambient air and the toxicities such as mutagenicity in the fluid, could work for the protection of health of worker in machine work shops, through an environmental control system.