Forensic Neuropathological Study of Fibroblast Growth Factor and Stress Protein

成纤维细胞生长因子和应激蛋白的法医神经病理学研究

• 批准号: 06670462
• 负责人: NAKASONO Ichiro
• 金额: $ 1.34万
• 依托单位: Nagasaki University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670462/
• 关键词: Fibroblast growth factor; Stress protein; Immunohistochemistry; Forensic autopsied brain

1.Immunohistochemically, heat shock proteins, HSP72 and ubiquitin, were demonstrated in 36 cases of forensic autopsy : asphyxia cases, trauma cases, SIDS,intoxication and others. In cases of asphyxia, alcohol-, tolen-and drug-intoxication, HSP72 could be observed in the cytoplasm of nerve cells of hippocampus. These results suggest that HSP in cells and tissues reflect injuries as the cause of death. In forensic autopsy, detection of HSP in affected organs is considered to be very useful for understanding the cause and mechanism of death.2.A neuropathological study of 47 forensic autopsy cases (hypoxic/ischemic brain damage) has been undertaken, using immunohistochemical staining to detect the HSP and FGF.In cases surviving 2-5h after hypoxic/ischemic injury, ischemic changes were seen, and the remained neurons showed immunoactivitiy of a-FGF and b-FGF.But glial reactions were not apparent. In cases of longer survival, neuronal necrosis and loss of neurons were seen, and these changes were accompanied by proliferation of glial fibrillary acidic protein (GFAP) , vimentin-positive and a-FGF-positive astrocytes.3.Neuropathologically, pathological changes of diffuse axonal injury (DAI) was examined in 18 cases of closed head unjury with local injuries. In 11 cases (61.1%) , axonal and myeline degeneration and retraction balls could be observed. To make clear the neuronal changes in DAI,hippocampus of those cases was examined. Immunohistochemistrically, MAP,EK,VIP,HSP,FOS and FGF were observed in CA2-CA4 area in hippocampus. But in CA1 area those stainabilities were decreased. Therefore, pyramidal cells in CA1 shows not only morphological change but also functional degenerations. On the other hand, pyramidal cells in CA2-CA4 have some protecting and repairing mechanism because it is considered that VIP,HSP72, c-FOS and b-FGF have effects to protect and repair against the degenerations of nerve cells.

1. 法医尸检36例：窒息病例、外伤病例、SIDS、中毒等，免疫组化显示热休克蛋白、HSP72和泛素。窒息、酒精、甲苯、药物中毒时，海马神经细胞胞质中可观察到HSP72。这些结果表明细胞和组织中的 HSP 反映了损伤是死亡原因。在法医尸检中，检测受累器官中的HSP被认为对于了解死亡原因和机制非常有用。2.对47例法医尸检病例（缺氧/缺血性脑损伤）进行了神经病理学研究，利用免疫组化染色检测HSP和FGF。缺氧/缺血性损伤后存活2-5小时的病例可见缺血性改变，并且 其余神经元均表现出a-FGF和b-FGF的免疫活性，但胶质细胞反应不明显。生存期较长的病例可见神经元坏死和神经元丢失，并伴有胶质原纤维酸性蛋白（GFAP）、波形蛋白阳性和a-FGF阳性星形胶质细胞的增殖。 3.神经病理学：18例局部损伤的闭合性头部损伤中出现弥漫性轴索损伤（DAI）病理改变。 11例（61.1%）可见轴突、髓鞘变性及球回缩。为了明确DAI中神经元的变化，对这些病例的海马体进行了检查。免疫组化检测海马CA2-CA4区MAP、EK、VIP、HSP、FOS、FGF。但在 CA1 区域，这些染色能力下降。因此，CA1区锥体细胞不仅表现出形态变化，而且表现出功能退化。另一方面，CA2-CA4中的锥体细胞具有一定的保护和修复机制，因为认为VIP、HSP72、c-FOS和b-FGF具有保护和修复神经细胞变性的作用。

项目成果

折原義行: "新旧の硬膜下出血(血腫)を認めたシェ-グレン症候群の1剖検例" 日本法医学雑誌. 48. 452-456 (1994)

Yoshiyuki Orihara：“伴有新旧硬膜下出血（血肿）的干燥综合征尸检病例”《日本法医学杂志》48. 452-456（1994）。

久保 真一: "法医剖検例における熱ショック蛋白質HSP72ならびにUbiquitinの出現様態" 法医学の実際と研究. 37. 159-168 (1994)

久保真一：“法医尸检病例中热休克蛋白 HSP72 和泛素的出现”法医学实践与研究 37. 159-168 (1994)。

Shin-ichi Kubo: "Immuno his tological investigations of auto piced carotid bosies and their application to oiagnosing stranga lation" Int J Leg Med. 106. 281-284 (1994)

Shin-ichi Kubo：“对自体颈动脉的免疫组织学研究及其在诊断绞杀中的应用”Int J Leg Med。

久保真一: "Waterhoese-Fridenichsen 症候群の1剖検例" 法医学の実際と研究. 37. 329-333 (1994)

Shinichi Kubo：“Waterhoese-Fridenichsen 综合征的尸检案例”《法医实践与研究》37. 329-333 (1994)。

O.Kitamura: "Immunohistochemical investigation of buyoxicl is chemic Drain damage in forensic autopsy cases" Int J Leg Med. 107. 69-76 (1994)

O.Kitamura：“Buoxicl 的免疫组织化学研究是法医尸检案例中的化学引流损伤”Int J Leg Med。