Forensic Neuropathological Study of Fibroblast Growth Factor and Stress Protein

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1.Immunohistochemically, heat shock proteins, HSP72 and ubiquitin, were demonstrated in 36 cases of forensic autopsy : asphyxia cases, trauma cases, SIDS,intoxication and others. In cases of asphyxia, alcohol-, tolen-and drug-intoxication, HSP72 could be observed in the cytoplasm of nerve cells of hippocampus. These results suggest that HSP in cells and tissues reflect injuries as the cause of death. In forensic autopsy, detection of HSP in affected organs is considered to be very useful for understanding the cause and mechanism of death.2.A neuropathological study of 47 forensic autopsy cases (hypoxic/ischemic brain damage) has been undertaken, using immunohistochemical staining to detect the HSP and FGF.In cases surviving 2-5h after hypoxic/ischemic injury, ischemic changes were seen, and the remained neurons showed immunoactivitiy of a-FGF and b-FGF.But glial reactions were not apparent. In cases of longer survival, neuronal necrosis and loss of neurons were seen, and these changes were accompanied by proliferation of glial fibrillary acidic protein (GFAP) , vimentin-positive and a-FGF-positive astrocytes.3.Neuropathologically, pathological changes of diffuse axonal injury (DAI) was examined in 18 cases of closed head unjury with local injuries. In 11 cases (61.1%) , axonal and myeline degeneration and retraction balls could be observed. To make clear the neuronal changes in DAI,hippocampus of those cases was examined. Immunohistochemistrically, MAP,EK,VIP,HSP,FOS and FGF were observed in CA2-CA4 area in hippocampus. But in CA1 area those stainabilities were decreased. Therefore, pyramidal cells in CA1 shows not only morphological change but also functional degenerations. On the other hand, pyramidal cells in CA2-CA4 have some protecting and repairing mechanism because it is considered that VIP,HSP72, c-FOS and b-FGF have effects to protect and repair against the degenerations of nerve cells.