The establishment of new marker to diagnose the wound age

诊断伤口年龄的新标记物的建立

• 批准号: 21390216
• 负责人: NAKASONO Ichiro
• 金额: $ 11.48万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390216/
• 关键词: 法医病理学; 皮膚損傷; 蛋白質; 受傷時期推定; 法医剖検例; 損傷; マクロファージ; CD14; c-fos; fosB; マウス; 皮膚

RNA analysis has been applied to forensic work to determine wound age. We already investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1(MKP-1), CD14, chemokine(C-C motif) ligand 9(CCL9), placenta growth factor(PlGF), mast cell protease-5(MCP-5), growth arrest specific 5(Gas5), beta-2 microglobulin(B2M) and major urinary protein-1(MUP-1), in terms of repair response in adult mice of wound age. In this study, we focused on the CD14 protein expression during wound healing. We demonstrated that the CD14 protein only expressed from 3 to 5 day after wound with immunohistological and western blot method. In addition, the CD14 specifically expressed at macrophage around wound. These results indicated that investigation of CD 14 protein must be useful of estimation for wound age in forensic practice.

RNA分析已应用于法医工作，以确定伤口的年龄。我们已经使用定量RT-PCR研究了10个基因的mRNA表达，包括c-fos，fosB，丝裂原活化蛋白激酶磷酸酶-1（MKP-1），CD 14，趋化因子（C-C基序）配体9（CCL 9）、胎盘生长因子（PlGF）、肥大细胞蛋白酶-5（MCP-5）、生长停滞特异性5（Gas 5）、β-2微球蛋白（B2 M）和主要尿蛋白-1（MUP-1），在创伤年龄的成年小鼠的修复反应方面。在本研究中，我们重点研究了创伤愈合过程中CD 14蛋白的表达。免疫组化和western blot检测结果显示，创伤后3 ~ 5天，CD 14蛋白表达增加。另外，CD 14在创面周围巨噬细胞上特异性表达。结果表明，检测CD 14蛋白对法医学上推断创伤时间有一定的参考价值。