The expression of a Novel Stress Protein "150-kD a Oxygen Regulated Protein (ORP-150) "in Forensic Autopsy Cases

新型应激蛋白“150-kD氧调节蛋白（ORP-150）”在法医尸检案例中的表达

• 批准号: 14370156
• 负责人: NAKASONO Ichiro
• 金额: $ 9.09万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370156/
• 关键词: ORP-150; Stress Protein; Sudden Infant Death; Forensic Autopsy Case; Human; 法医培検脳

The oxygen regulated protein 150-kDa (ORP-150) is only induced in hypoxic conditions. We performed an immunohistochemical and morphometrical study on the expression of ORP-150 in the brains of sudden infant death (SID) victims. The cerebral cortexes of 18 infants were used for this study. Each tissue section was incubated with anti-ORP-150 polyclonal antibodies and the number of ORP-150 positive cells was counted. In the cluster analysis, the 18 cases were classified into three groups (A-C groups). Group A was composed of 6 sudden infant death syndrome (SIDS) cases and its mean value of ORP-150 positive cells was 66.75+/-3.44, Group B (6 severe respiratory infectious disease such as pneumonia and bronchitis including sepsis) : 39.50+/-2.52 and Group C (5 SIDS and 1 severe respiratory infectious disease) 16.00+/-2.92, respectively. These results might reflect chronic hypoxic condition before death, because ORP-150 is only induced when a hypoxic condition exist, but not acute hypoxia. An … More d chronic hypoxic state is likely to be antecedent to SIDS. Therefore, immunohistochemical analysis of OPR-150 in the brain of SID cases may be very useful to differentiate between SIDS and acute asphyxia. Moreover, we examined the expression of ORP-150 in the adult samples, and discussed its significance in forensic practice. The cerebral cortexes of 31 cases (asphyxia 9 cases, hypothermia : 4, exsanguinations 5, CO intoxication (CO) : 6, sudden cardiac death (SCD): 7) were used for this study. The same immunohistolical method was used. In the multiple linear regression method, the estimated regression coefficient of ORP-150 on age was significant (p0.039), thus, we could find that the ORP-150 expression level depended on age. Using analysis of covariance, we compared the means of ORP-150, LS1VIEAN, which means hypothetic average value excluding influence of age, for each cause of death. The LSMEAN +/-S.E. was 84.74+/-9.03 in hypothermia, 57.52+/-6.34 in asphyxia, 46.68+/-6.70 in CO, 24.84+/-8.05 in exsanguinations, and 16.24+/-7.35 in SCD. As a result of the analysis, the LSMEAN of the ORP-150 expression level was related to the cause of death. There might be differences in the duration of brain ischemia before death. For example, SCD is presumed to be instant death, while brain ischemia continues for several minutes in asphyxia, CO and exsanguinations, and for several hours in hypothermia cases. Therefore, the immunohistochemical and morphometrical analysis of ORP-150 in the brain may be very useful to determine the duration of brain ischemia before death in forensic autopsy cases. Less

氧调节蛋白150 kDa(ORP-150)只有在低氧条件下才能被诱导。我们对婴儿猝死(SID)患者脑组织中ORP-150的表达进行了免疫组织化学和形态计量学研究。本研究以18例婴儿大脑皮层为研究对象。各组织切片用抗ORP-150多克隆抗体孵育，计数ORP-150阳性细胞数。在聚类分析中，将18例患者分为A-C组。其中A组6例为婴儿猝死综合征，其ORP-150阳性细胞平均值为66.75±-3.44，B组(肺炎、支气管炎、脓毒症等6例严重呼吸道传染病)为39.50+/-2.52，C组(5例SIDs和1例严重呼吸道传染病)为16.00±2.92。这些结果可能反映了生前的慢性低氧状态，因为ORP-150只有在低氧状态下才被诱导，而不是急性低氧状态。一个…更多的慢性低氧状态可能是SIDS的先兆。因此，对SID患者脑组织中OPR-150的免疫组织化学分析有助于鉴别SID和急性窒息。此外，我们还检测了ORP-150在成人标本中的表达，并讨论了其在法医实践中的意义。本组共31例，其中窒息9例，体温过低4例，失血5例，一氧化碳中毒6例，心源性猝死7例。采用相同的免疫组织化学方法。在多元线性回归方法中，ORP-150对年龄的估计回归系数达到显著水平(p0.039)，由此可以发现ORP-150的表达水平与年龄有关。采用协方差分析，比较各死因的ORP-150均值、LS1VIEAN均值(即排除年龄影响的假设平均值)。低温组LSMEAN+/-9.03+/-9.03，窒息57.52+/-6.34，CO 46.68+/-6.70，出血性24.84+/-8.05，SCD 16.24+/-7.35。分析结果显示，ORP-150表达水平的LSMEAN与死亡原因有关。死亡前的脑缺血持续时间可能会有所不同。例如，SCD被认为是即刻死亡，而脑缺血在窒息、CO和失血时持续几分钟，在体温过低的情况下持续几个小时。因此，在法医尸检案例中，脑内ORP-150的免疫组织化学和形态计量学分析可能对确定脑缺血死亡前的持续时间非常有用。较少

项目成果

Ikematsu et al.: "The Expression of "150-kDa Oxygen Regulated Protein (ORP-150)" in Human Brain and its relationship with duration time until death"Legal Medicine. 6(2). 97-101 (2004)

Ikematsu 等人：“人脑中“150-kDa 氧调节蛋白（ORP-150）”的表达及其与死亡持续时间的关系”法律医学。

Ikematsu et al.: "The Expression of "150-kDa Oxygen Regulated Protein (ORP-150)" in Human Brain and its relationship with duration time until death"Legal Medicine. (In press).

Ikematsu 等人：“人脑中“150-kDa 氧调节蛋白（ORP-150）”的表达及其与死亡持续时间的关系”法律医学。

Ikematsu et al.: "The expression of a novel stress protein '150-kDa oxygen regulated protein' in sudden infant death"Legal Medicine. 5(1). 15-19 (2003)

Ikematsu 等人：“婴儿猝死中新型应激蛋白‘150-kDa 氧调节蛋白’的表达”法律医学。

Ikematsu et al.: "The Expression of a Novel Stress Protein "150-kDa Oxygen Regulated Protein (ORP-150) "in Sudden Infant Death""Legal Medicine. (in press).

Ikematsu 等人：“新型应激蛋白“150-kDa 氧调节蛋白（ORP-150）在婴儿猝死中的表达”“法律医学”（正在出版）。