Effects of abnormalities of adhesion molecule on T-cell function in HIV-infected patients.

(1)检测HIV感染血友病患者淋巴细胞和单核细胞的黏附分子：β1(VLA-β1)(CD29)、α4(VLA-α4)(CD49d)、α6(VLA-α6)(CD49f)、αL(LFA-1α)(CD11a)、β3(CD61)、β7、L-选择素(CD62L)、细胞间黏附分子-1(CD54)以及人类白细胞抗原-DR、CD69、CD30、CD71、CD45RO、CD26、β2-微球蛋白。以Thy1，2为阴性对照。HIV感染者外周血中CD_4、CD_8β_3(CD61)显著降低(P<0.01)。本研究中T淋巴细胞β3(CD61)低表达的原因及临床意义尚不清楚。(2)6例白血病细胞浸润性皮肤ATL细胞中3例表达CLA抗原(E-选择素)。3例白血病浸润至肠道的ATL细胞中2例表达整合素β7。(3)ATL细胞可通过LFA-1/ICAM-1同型黏附和钙依赖性黏附、黏附分子的信号转导、细胞因子的产生和增殖等多种途径进行增殖。

(1) The adhesion melecules, that is, beta1 (VLA-beta1) (CD29), alpha4 (VLA-alpha4) (CD49d), alpha6 (VLA-alpha6) (CD49f), alphaL (LFA-1alpha) (CD11a), beta3 (CD61), beta7, L-selectin (CD62L), ICAM-1 (CD54) of lymphocytes and monocytes were measured as well as HLA-DR,CD69, CD30, CD71, CD45RO,CD26, beta2-micro-globulin in HIV-infected patients with hemophilia. Thy1,2 was used as negative control. beta3 (CD61) of CD4 and CD8 were significantly lowered (p<0.01) in HIV-infected patients. In this study, the reason and clinical significance of low level of beta3 (CD61) of T lymphocytes could not clarified.(2) ATL cells (3 of 6 cases) from the patients with leukemic cell infiltration to skin expressed CLA antigen (legand of E-selectin). ATL cells (2 of 3 cases) from the patients with leukemic infiltration to gut expressed integrin beta7. The results suggested that the express of adhesion molecules on leukemic cells play a role in determination of ATL cells infiltration to tissure.(3) ATL cells may proliferate in the sequentioal manner, such as, homotypic and calcium-dependent adhesion through LFA-1/ICAM-1, the signal transduction throug hthese adhesion molecules, the production of cytokines and the proliferation.