Study on the collagen expression related with the rates of synthesis and degradation during the course of hepatic fibrosis

肝纤维化过程中胶原表达与合成和降解速率相关的研究

基本信息

  • 批准号:
    06670559
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

The amount of collagen deposited must depend on the balance between the rates of synthesis and degradation. Our objectives were to study the gene expression of type I and III procollagens, metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1), to determine type I and III collagen immunolocalization, and to assay hepatic collagen content during the course of hepatic fibrosis induced in rats by administration of dimethylnitrosamine (DMN), whereas PS-induced fibrosis showed the preponderance of type I collagen gene expression and immunodeposition. Fibrosis was greatest and type III procollagen gene expression highest on day 14 after DMN treatment and 10 weeks after PS treatment, when type III collagen immunodeposition predominated. DMN increased hepatic collagen contents dose-dependently ; type III procollagen mRNA levels increased on day 14. Relative MMP-1 mRNA levels increased early in hepatic fibrogenesis and with low DMN dosages on day 14. Decreased MMP-1 mRNA levels with high DMN dosages increased collagen content and caused hepatic fibrosis. Fibrotic liver collagen content may thus first notably increase partly due to the balance between type I collagen and MMP-1 expression retes, and type I or III collagen expression may play a role in collagen accumulation according as pathogenetic circumstances.
胶原沉积的数量必须取决于合成和降解速率之间的平衡。我们的目的是研究二甲基亚硝胺(DMN)诱导大鼠肝纤维化过程中I型和III型前胶原、金属蛋白酶-1 (MMP-1)和金属蛋白酶-1组织抑制剂(TIMP-1)的基因表达,确定I型和III型胶原的免疫定位,并测定肝胶原含量,而ps诱导的纤维化显示I型胶原基因表达和免疫沉积优势。DMN治疗后第14天和PS治疗后第10周纤维化程度最大,III型前胶原基因表达最高,此时以III型胶原免疫沉积为主。DMN增加肝胶原含量呈剂量依赖性;第14天III型前胶原mRNA水平升高。MMP-1 mRNA的相对水平在肝纤维化早期和低DMN剂量的第14天升高。高剂量DMN降低MMP-1 mRNA水平,增加胶原含量,引起肝纤维化。因此,纤维化肝胶原含量可能首先显著增加,部分原因是I型胶原和MMP-1表达通路之间的平衡,根据发病情况,I型或III型胶原的表达可能在胶原积累中起作用。

项目成果

期刊论文数量(45)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yasuda M: "Estradiol suppresses liver fibrogensis" Gut. 37 (Suppl 2). A78- (1995)
安田 M:“雌二醇抑制肝纤维化”Gut。
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    0
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柴昌子: "ジメチルニトロサミン線維肝と肝硬変患者における寒冷疼痛負荷試験に対するブラデイキニン反応." 消化管ホルモン(X III)(消化管ホルモン研究会編)医学図書出版社、東京. 259-264 (1995)
Masako Shiba:“二甲基亚硝胺纤维化和肝硬化患者的缓激肽对冷痛应激测试的反应。”胃肠激素(X III)(由胃肠激素研究组编辑)Igaku Tosho Publishing,东京259-264。
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    0
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Shiba M: "Collagen accumulation before histological changes in hepatic fibrosis" Gut. 37 (Supple 2) : A78.(1995)
Shiba M:“肝纤维化组织学变化前的胶原蛋白积累”Gut。
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    0
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Shimizu I: "Radioimmunoreactive plasma bradykinin levels and histological changes during the course of caerulein-induced pancreatitis in rats." Pancreas. 8. 220-225 (1993)
Shimizu I:“在雨蛙素诱导的大鼠胰腺炎过程中,放射免疫反应性血浆缓激肽水平和组织学变化。”
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    0
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Shimizu I: "Radioimmunoreactive plasma bradykinin levels and histological changes during the course of caerulein-induced pancreatitis in rats" Pancreas. 8 (2). 220-225 (1993)
Shimizu I:“大鼠雨蛙素诱导的胰腺炎过程中放射免疫反应性血浆缓激肽水平和组织学变化”胰腺。
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    0
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SHIMIZU Ichiro其他文献

SHIMIZU Ichiro的其他文献

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{{ truncateString('SHIMIZU Ichiro', 18)}}的其他基金

Investigation of Deformation Mechanism Dependent Elastic-plastic Multi-axial Compressive Behavior and Constitutive Modeling of Metastable Beta-type Titanium Alloys
亚稳态β型钛合金变形机制相关弹塑性多轴压缩行为及本构模型研究
  • 批准号:
    24560101
  • 财政年份:
    2012
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of Forming Limit Criterion of Magnesium Alloys under Multiaxial Compressive Deformation at Cold and Elevated Temperatures
镁合金冷高温多轴压缩变形极限准则的研究
  • 批准号:
    21560094
  • 财政年份:
    2009
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Twin-dominated Hardening Behavior of Titanium Materials during Cold/Warm Non-Proportional Biaxial Compressions
钛材料在冷/温非比例双轴压缩过程中的孪晶主导硬化行为
  • 批准号:
    19560092
  • 财政年份:
    2007
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The efficacy of the suppressort CD8 T cells for cardiac transplantation
抑制型CD8 T细胞在心脏移植中的功效
  • 批准号:
    19890154
  • 财政年份:
    2007
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Young Scientists (Start-up)
Combined gene therapy with oncolytic adenovirus and NK-4 against tumor growth and invasion in hepatocellular carcinoma
溶瘤腺病毒和 NK-4 联合基因治疗对抗肝细胞癌肿瘤生长和侵袭
  • 批准号:
    18590738
  • 财政年份:
    2006
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Oxidative stress is a potent link between hepatocyte damage and HSC activation in hepatic fibrosis
氧化应激是肝纤维化过程中肝细胞损伤和 HSC 激活之间的重要联系
  • 批准号:
    11670509
  • 财政年份:
    1999
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of polarized laser scanning microscope and its use to quantitative analysis of rock textures
偏光激光扫描显微镜的研制及其在岩石结构定量分析中的应用
  • 批准号:
    09440173
  • 财政年份:
    1997
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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IVA族磷脂酶A2在高脂饮食喂养小鼠恢复正常饮食后肝纤维化中的作用
  • 批准号:
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通过护理测试开发来识别有日本血吸虫相关肝纤维化进展风险的个体的生物标志物
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    10632049
  • 财政年份:
    2022
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通过护理测试开发来识别有日本血吸虫相关肝纤维化进展风险的个体的生物标志物
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    10434390
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阐明 Galectin-3 相互作用以破坏肝纤维化
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将脂肪代谢与肝纤维化联系起来
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Tenascin-X 在肝纤维化进展中的作用和新疗法的开发
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血管内皮功能治疗抑制肝纤维化的效果评价
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