USEFULNESS OF LIQUID VENTILATION BY PERFLUOROCARBON IN EXEPIMENTAL MODEL OF ACUTELUNG INJURY

本实验观察了全氟碳液(Liqui Vent；LV)对体重10~15 kg麻醉绵羊内毒素血症所致急性肺损伤的影响。我们先前已经准备了肺淋巴瘘和血流动力学监测。将10ug/kg的大肠杆菌内毒素经气管插管注入肺内，1h后静注。实验方案：(1)麻醉条件：FiO2 0.5，TV 12ml/kg，RR 30/min，PEEP 5cmH2O LV；10 mI/kg(2)麻醉条件；FiO2 1.0，TV 12ml/kg，RR 30/min，PEEP 5cmH2O LV；实验(1)肺内注射LV可部分抑制内毒素所致的肺动脉高压、肺淋巴流量增加、低氧血症和外周血白细胞减少。但在实验(2)中，LV滴注并不能抑制内毒素引起的上述改变，FIO2和LV体积的不同可能导致实验(1)和(2)的结果不同。我们还需要进一步的研究来观察全氟碳液体通气对急性肺损伤的影响。

We investigated the effects of liquid ventilation using perfluorocarbon (Liqui Vent ; LV) on acute lung injury induced by endotoxemia in anesthetized sheep weighing 10-15 kg. We had previously prepared the lung lymph fistula and hemodynamic monitering. E.coli endotoxin of 10 ug/kg was infused i. v. 1 hr after LV instillation into the lungs through the trachea tube. Anesthesia was continued by intravenous pentobarbital sodium of 25 mg/kg and panclonium of 0.1 mg/kg.Measurements : Pulmonary hemodynamics, systemic hemodynamics, lung lymph balance, arterial blood gas analysis, peripheral leukocyte countsExperimental protocol :(1)Anesthesia condition ; Fio2 0.5, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 10 mi/kg(2)Anesthesia condition ; FiO2 1.0, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 20 ml/kgIn each experiment sheep were divided into the endotoxin alone and the endotoxin plus LV group.Results are as follows. In experiment (1) , pulmonary hypertension, increased lung lymph flow, hypoxemia and peripheral leukopenia induced by endotoxin were partially suppressed by pretreatment of LV instillation into the lungs. However in experiment (2) these changes induced by endotoxin were not suppressed by LV instillation.The differences of FiO2 and the volume of LV might produce the different results between experimental (1) and (2) . We need further studies to see the effects of the liquid ventilation by perfluorocarbon on acute lung injury.