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USEFULNESS OF LIQUID VENTILATION BY PERFLUOROCARBON IN EXEPIMENTAL MODEL OF ACUTELUNG INJURY

全氟碳液体通气在急性肺损伤实验模型中的用途

基本信息

批准号：
06670606
负责人：
KUBO Keishi
金额：
$ 1.15万
依托单位：
SHINSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

We investigated the effects of liquid ventilation using perfluorocarbon (Liqui Vent ; LV) on acute lung injury induced by endotoxemia in anesthetized sheep weighing 10-15 kg. We had previously prepared the lung lymph fistula and hemodynamic monitering. E.coli endotoxin of 10 ug/kg was infused i. v. 1 hr after LV instillation into the lungs through the trachea tube. Anesthesia was continued by intravenous pentobarbital sodium of 25 mg/kg and panclonium of 0.1 mg/kg.Measurements : Pulmonary hemodynamics, systemic hemodynamics, lung lymph balance, arterial blood gas analysis, peripheral leukocyte countsExperimental protocol :(1)Anesthesia condition ; Fio2 0.5, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 10 mi/kg(2)Anesthesia condition ; FiO2 1.0, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 20 ml/kgIn each experiment sheep were divided into the endotoxin alone and the endotoxin plus LV group.Results are as follows. In experiment (1) , pulmonary hypertension, increased lung lymph flow, hypoxemia and peripheral leukopenia induced by endotoxin were partially suppressed by pretreatment of LV instillation into the lungs. However in experiment (2) these changes induced by endotoxin were not suppressed by LV instillation.The differences of FiO2 and the volume of LV might produce the different results between experimental (1) and (2) . We need further studies to see the effects of the liquid ventilation by perfluorocarbon on acute lung injury.

我们研究了使用全氟化碳液体通气 (Liqui Vent ; LV) 对体重 10-15 kg 的麻醉绵羊由内毒素血症引起的急性肺损伤的影响。我们之前准备了肺淋巴瘘和血流动力学监测。静脉注射10ug/kg大肠杆菌内毒素。 v. LV 通过气管注入肺部后 1 小时。继续麻醉，静脉注射戊巴比妥钠25mg/kg，泮克隆0.1mg/kg。测量：肺血流动力学、全身血流动力学、肺淋巴平衡、动脉血气分析、外周血白细胞计数。实验方案：（1）麻醉条件； Fio2 0.5，TV 12ml/kg，RR 30/min，PEEP 5cmH2O LV ； 10 mi/kg(2)麻醉条件； FiO2 1.0，TV 12ml/kg，RR 30/min，PEEP 5cmH2O LV ； 20ml/kg各实验将羊分为单独内毒素组和内毒素加LV组。结果如下。在实验(1)中，左心室滴入肺部的预处理部分抑制了内毒素引起的肺动脉高压、肺淋巴流量增加、低氧血症和外周白细胞减少。然而，在实验(2)中，内毒素引起的这些变化并没有被左心室滴注所抑制。FiO2和左心室体积的差异可能会产生实验(1)和(2)之间不同的结果。我们需要进一步的研究来了解全氟化碳液体通气对急性肺损伤的影响。