Multifactorial analysis of chronicity and pathogenesis in patients with sarcoidosis

结节病慢性病程及发病机制的多因素分析

基本信息

  • 批准号:
    06670610
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

The clinical features of sarcoidosis are heterogenous in different ethnic groups, suggesting that different genetic or environmental backgrounds influences the disease. For comparison between Japanese and Swedish in whom a correlation between lung accumulated CD4 Tcells expressing the T cell receptor (TCR) V_<alpha> 2.3 gene segment and a particular HLA type (DR3 (17), DQ2) was shown, we examined TCR V gene usage and gammadelta TCR expression in CD4 and CD8 T cells in BAL fluid and peripheral blood obtained from Japanese sarcoidosis patients and healthy controls. We used a panel of 13 monoclonal antibodies (Mab) specific for different TCR V genes, which altogether stained approximately 50% of the T cells, and triple staining techniques with flow cytometry. The patients and controls were also HLA-typed. As results, a high degree of expression of gammadelta TCR in blood T cells of close to half of the patients. Expansion of T cell subsets was readily detected in the CD8 T cell population, while a more homogenous staining pattern was found in the CD4 T cell population. On the other hand, the pattern of reactivities found in CD4 and CD8 subsets of blood lymphocytes of Japanese healthy subjects was in general found to be match that in healthy Caucasian subjects. It remains to be discussed whether influence of ethnic factors on TCR gene expression of lung T cells is greater than that of environmental ones, in the case of diseases processes such as sarcoidosis.
结节病的临床特征在不同种族群体中是异质性的,这表明不同的遗传或环境背景影响疾病。为了比较日本人和瑞典人肺内聚集的表达T细胞受体(TCR)V_ 2.3基因片段的CD4 T细胞<alpha>与特定HLA类型(DR 3(17),DQ 2)之间的相关性,我们检测了日本结节病患者和健康对照者BAL液和外周血中CD4和CD8 T细胞的TCR V基因使用和γ δ TCR表达。我们使用了一组13种特异于不同TCR V基因的单克隆抗体(Mab),共染色约50%的T细胞,以及流式细胞术的三重染色技术。患者和对照组也进行了HLA分型。结果,接近一半的患者的血液T细胞中高度表达γ δ TCR。在CD8 T细胞群中容易检测到T细胞亚群的扩增,而在CD4 T细胞群中发现更均匀的染色模式。另一方面,日本健康受试者血液淋巴细胞CD 4和CD 8亚群中发现的反应模式总体上与健康高加索受试者相匹配。在结节病等疾病过程中,种族因素对肺T细胞TCR基因表达的影响是否大于环境因素,还有待讨论。

项目成果

期刊论文数量(41)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shigematsu M.: "T cell receptor (TCR) V gene segment use in HLA-typed Japanese healthy subjects." Clin Exp Immunol. 103-2. 149-154 (1996)
Shigematsu M.:“T 细胞受体 (TCR) V 基因片段在 HLA 型日本健康受试者中的使用。”
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    0
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  • 通讯作者:
Tsutsumi T et al: "Soluble interleukin-2 receptor in blood from patients with sarocidosis and idiopathic pulmonary fibrosis" Sarcoidosis. 11. 102-109 (1994)
Tsutsumi T 等人:“结节病和特发性肺纤维化患者血液中的可溶性白细胞介素 2 受体”结节病。
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    0
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  • 通讯作者:
Nagai S.: "Pulmonary Sarcoidosis; Population differences and pathophysiology." Southern Med J.88-10. 1001-1010 (1995)
Nagai S.:“肺结节病;人群差异和病理生理学。”
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    0
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Guzman J: "ICAM-1 and integrin expression on isolated human alveolar type II pueumocytes" Eur Respin. J 7-6. 736-739 (1994)
Guzman J:“分离的人肺泡 II 型肺细胞上的 ICAM-1 和整合素表达”Eur Respin。
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    0
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  • 通讯作者:
Grunewald J et al: "T cell receptor V gene expression in HLA-typed Japanese patients with pulomonary sarcoidosis" Am J Respir Crit Med. 151. 151-156 (1995)
Grunewald J 等人:“日本 HLA 型肺结节病患者中 T 细胞受体 V 基因表达”Am J Respir Crit Med。
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    0
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NAGAI Sonoko其他文献

Cigarette smoke induced DNA damage, but not apoptosis of alveolar macrophages.
香烟烟雾会引起 DNA 损伤,但不会引起肺泡巨噬细胞凋亡。
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    HIRONO Yuriko;INOUE Shinichi;MIYAGAWA Mayuko;MAZDA Osamu;NAGAI Sonoko;TAKEUCHI Toru;PINKERTON K.E;TAKEUCHI Minoru
  • 通讯作者:
    TAKEUCHI Minoru

NAGAI Sonoko的其他文献

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{{ truncateString('NAGAI Sonoko', 18)}}的其他基金

Protective mechanisms in inflammatory lung diseases at airway and alveolar area
气道和肺泡区炎症性肺部疾病的保护机​​制
  • 批准号:
    12670557
  • 财政年份:
    2000
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Inflammation and Fibrosis in patients with Interstitial Pneumonia
间质性肺炎患者的炎症和纤维化
  • 批准号:
    10670540
  • 财政年份:
    1998
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathophysiological and Logical Clinical Study in patients with Sarcoidosm Prognoitic factors
结节病患者的病理生理学和逻辑临床研究 预后因素
  • 批准号:
    08670661
  • 财政年份:
    1996
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Characterization of IL-1 inhibitor released from alveolar macrophage and evaluation of chronic inflammation in lungs.
肺泡巨噬细胞释放的 IL-1 抑制剂的表征和肺部慢性炎症的评估。
  • 批准号:
    03670399
  • 财政年份:
    1991
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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