ANALYSIS OF ANTIGEN-SPECIFIC HELPER T CELL SUBSETS INVOLVED IN FOOD ALLERGY AND MODIFICATION OF T CELL RESPONSE TO ALLERGENS.

鸡蛋过敏症患儿外周血淋巴细胞（PBL）对鸡蛋白色、卵清蛋白和卵粘蛋白均产生增殖反应，产生IL-4的水平显著高于对照组。与PHA刺激诱导更多的IL-4产生细胞比对照组。特应性皮炎患者的PBL产生的IFN-γ少于孤立IgE介导的鸡蛋过敏患者的PBL。从特应性皮炎患者中建立的卵清蛋白特异性CD 4 ^+ T细胞克隆产生IL-4，但不产生IFN-γ或产生有限量的IFN-γ。克隆对obalbumin肽323-339有反应。肽PH-1.0（Leu-Tyr-Gln-Glu-Leu-Gln-Lys-Leu-Thr-Gln-Thr-Leu-Lys）抑制肽323-339与类淋巴母细胞系的结合，并以剂量依赖性方式抑制抗原呈递。

Peripheral blood lymphocytes (PBL) from child with egg hypersensitivity proliferated in response to egg white, ovalbumin as well as obomucoid, and produced significantly more IL-4 than PBL from control chidren. Stimulation with PHA induced more IL-4-producing cells than the control. PBL from patients with atopic dermatitis produced less IFN-gamma than PBL from patients with isolated IgE-mediated egg hypersensitivity. Ovalbumin-specific CD4^+ T cell clones established from patients with atopic dermatitis produced IL-4, but no, or a limited amount of IFN-gamma. A clone responded to obalbumin peptide 323-339. Peptide PH-1.0 (Leu-Tyr-Gln-Glu-Leu-Gln-Lys-Leu-Thr-Gln-Thr-Leu-Lys) suppressed the binding to the lymphoblastoid cell line of peptide 323-339 and inhibited antigen presentation in a dose dependent manner.