ANALYSIS OF ANTIGEN-SPECIFIC HELPER T CELL SUBSETS INVOLVED IN FOOD ALLERGY AND MODIFICATION OF T CELL RESPONSE TO ALLERGENS.

食物过敏相关抗原特异性辅助 T 细胞亚群的分析以及 T 细胞对过敏原反应的改变。

• 批准号: 06670773
• 负责人: TAKAGI Shuji
• 金额: $ 1.09万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670773/
• 关键词: EGG ALLERGY; CYTOKINES; T CELL CLONE; インターロイキン4; インターフェロン・ガンマ

Peripheral blood lymphocytes (PBL) from child with egg hypersensitivity proliferated in response to egg white, ovalbumin as well as obomucoid, and produced significantly more IL-4 than PBL from control chidren. Stimulation with PHA induced more IL-4-producing cells than the control. PBL from patients with atopic dermatitis produced less IFN-gamma than PBL from patients with isolated IgE-mediated egg hypersensitivity. Ovalbumin-specific CD4^+ T cell clones established from patients with atopic dermatitis produced IL-4, but no, or a limited amount of IFN-gamma. A clone responded to obalbumin peptide 323-339. Peptide PH-1.0 (Leu-Tyr-Gln-Glu-Leu-Gln-Lys-Leu-Thr-Gln-Thr-Leu-Lys) suppressed the binding to the lymphoblastoid cell line of peptide 323-339 and inhibited antigen presentation in a dose dependent manner.

鸡蛋过敏儿童外周血淋巴细胞（PBL）对蛋清、卵清蛋白和卵黏液样蛋白有增殖反应，产生的IL-4明显高于对照组儿童外周血淋巴细胞。与对照组相比，PHA刺激诱导了更多的产生il -4的细胞。来自特应性皮炎患者的PBL产生的ifn - γ少于来自孤立性ige介导的鸡蛋过敏患者的PBL。从特应性皮炎患者建立的卵白蛋白特异性CD4^+ T细胞克隆产生IL-4，但不产生，或产生有限数量的ifn - γ。克隆对白蛋白肽323-339有应答。肽PH-1.0 （leu - tir - gln - glu - leu - gln - lys - leu - thr - gln - thr - leu - lys）抑制肽323-339与淋巴母细胞样细胞系的结合，并呈剂量依赖性抑制抗原呈递。